A5067736631- Plant Gene Expression Analysis
- Enzyme Production and Characterization
- Microbial Metabolites in Food Biotechnology
- Microbial Natural Products and Biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Carbohydrate Chemistry and Synthesis
- Steroid Chemistry and Biochemistry
- Enzyme Catalysis and Immobilization
- Microbial bioremediation and biosurfactants
- Glycosylation and Glycoproteins Research
- Bacterial Genetics and Biotechnology
- Photosynthetic Processes and Mechanisms
- RNA and protein synthesis mechanisms
- Fungal Biology and Applications
Wroclaw University of Environmental and Life Sciences
2020-2025
Enzyme-based glycosylation is of great interest in the context natural products decoration. Yet, its industrial application hindered by optimisation difficulties and hard-to-standardise productivities. In this study, five sugar nucleotide-dependent glucosyltransferases from different origins (bacterial, plant fungal) were coupled with soy sucrose synthase (GmSuSy) to create a set diverse cascade biocatalysts for flavonoid glucosylation, which evaluation brought new insights into field....
Molecular and synthetic biology tools enable the design of new-to-nature biological systems, including genetically engineered microorganisms, recombinant proteins, novel metabolic pathways. These simplify development more efficient, manageable, tailored solutions for specific applications, biocatalysts, or biosensors that are devoid undesirable characteristics. The key aspect preparing these systems is availability appropriate strategies designing genetic circuits. However, there remains a...
Abstract Background The regioselective hydroxylation of phenolic compounds, especially flavonoids, is still a bottleneck classical organic chemistry that could be solved using enzymes with high activity and specificity. Yeast Rhodotorula glutinis KCh735 in known to catalyze the C-8 flavones flavanones. enzyme F8H (flavonoid C8-hydroxylase) involved reaction, but specific gene has not yet been identified. In this work, we present identification, heterologous expression characterization first...
Chalcones, including 4'-hydroxychalcones, have garnered significant attention in the area of drug discovery due to their diverse pharmacological properties, such as anti-inflammatory, antioxidative, and anticancer effects. However, low water solubility bioavailability limit efficacy
Abstract Glucosylation cascade consisting of Leloir glycosyltransferase and sucrose synthase with in situ regeneration system expensive low available nucleotide sugars is a game-changing strategy for enzyme-based production glycoconjugates relevant natural products. We designed stepwise approach including co-expression one-step purification co-immobilization on glass-based EziG resins from Glycine max ( Gm SuSy) promiscuous glucosyltransferase YjiC Bacillus licheniformis to produce...
Abstract Background Steroid compounds with a 6,19-oxirane bridge possess interesting biological activities including anticonvulsant and analgesic properties, bacteriostatic activity against Gram-positive bacteria selective anti-glucocorticoid action, while lacking mineralocorticoid progestagen activity. Results The study aimed to obtain new derivatives of 3β-acetyloxy-5α-chloro-6,19-oxidoandrostan-17-one by microbial transformation. Twelve filamentous fungal strains were used as catalysts,...
Abstract Flavin-dependent monooxygenases (FMOs) are a valuable group of biocatalysts that can regioselectively introduce hydroxy for the targeted modification biologically active compounds. Here, we present fdeE, FMO from Herbaspirillum seropedicae SmR1 is part naringenin degradation pathway and towards wide range flavonoids—flavanones, flavones, isoflavones, flavonols. Bioinformatics biochemical analysis revealed high similarity between analyzed enzyme other F8H FMOs what might indicate...
Flavonoids are a large and important group of phytochemicals with great variety bioactivities. The addition methyl groups during biosynthesis flavonoids other polyphenols enhances their bioactivities increases stability. In previous study our research group, we detected novel flavonoid O-methyltransferase activity in Streptomyces albidoflavus J1074, which led to the heterologous homohesperetin from hesperetin feeding cultures. this study, identify responsible for generation methylated...