- Food composition and properties
- Polysaccharides Composition and Applications
- Bacterial Genetics and Biotechnology
- Microbial Metabolites in Food Biotechnology
- Microbial Metabolic Engineering and Bioproduction
- Genomics and Phylogenetic Studies
- Enzyme Catalysis and Immobilization
- Microbial infections and disease research
- Bacteriophages and microbial interactions
- Photosynthetic Processes and Mechanisms
- Gene Regulatory Network Analysis
- Biofuel production and bioconversion
- Phytoestrogen effects and research
- Phytochemical and Pharmacological Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Microbial Community Ecology and Physiology
- Food Quality and Safety Studies
- Microbial bioremediation and biosurfactants
- Plant Gene Expression Analysis
- Enzyme Production and Characterization
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy
2023-2024
Polish Academy of Sciences
2023-2024
Wroclaw University of Environmental and Life Sciences
2013-2023
Medical University of Warsaw
2022
University of Wrocław
2021
Modular cloning has become a benchmark technology in synthetic biology. However, notable disparity exists between its remarkable development and the need for standardization to facilitate seamless interoperability among systems. The field is thus impeded by an overwhelming proliferation of organism-specific systems that frequently lack compatibility. To overcome these issues, we present Golden Standard (GS), Type IIS assembly method underpinned European Vector Architecture. GS unlocks...
Rothia is an opportunistic pathogen, particularly life-threatening for the immunocompromised. It associated with pneumonia, endocarditis, peritonitis and many other serious infections, including septicemia. Of note, mucilaginousa produces metabolites that support increase overgrowth of Pseudomonas aeruginosa, one ESKAPE bacteria. Endolysins are considered as antibacterial enzymes derived from bacteriophages selectively efficiently kill susceptible bacteria without harming human cells or...
ABSTRACT Modular cloning assembly has become a benchmark technology in synthetic biology. However, there is mismatch between its impressive development and the standardization required to promote interoperability different systems available. The full of field thus hampered by surge oftentimes incompatible organism-specific systems. To overcome these issues, we present Golden Standard (GS), Type IIS method underpinned European Vector Architecture (SEVA). GS unlocks modular applications with...
This study aimed to compare properties of retrograded starch acetates with an identical degree substitution, but produced from raw materials various botanical origin. Retrograded was potato, wheat, corn, and tapioca starch, afterwards acetylated acetic acid anhydride, adjusting reagent doses achieve esterification the modified preparation (2.1 g/100 g). Preparations differed significantly in their properties, which due disparate origin starch. The highest susceptibility acetylation...
Preparations from Hippophaë rhamnoides L. (sea buckthorn) have been traditionally used in the treatment of skin and digestive disorders, such as gastritis, gastric duodenal ulcers, uterine erosions, well oral, rectal, vaginal mucositis, particular Himalayan Eurasian regions. An influence an aqueous extract fruits H. (HR) on leakage lipopolysaccharide (LPS) Escherichia coli through gut epithelium developed human colorectal adenocarcinoma (Caco-2) monolayer vitro glucose transporter 2 (GLUT2)...
This study aimed to determine the effect of “annealing” acetylated potato starch with a homogenous granule size and various degrees substitution on thermal pasting characteristics (DSC), resistance amylases, rheology prepared pastes, swelling power dynamics drug release. A fraction large granules was separated from native sedimentation method doses acetic anhydride (6.5, 13.0 or 26.0 26 cm3/100 g starch). The acetates were then annealed at slightly lower temperatures than their temperatures....
Agriculture by-products were applied to proliferate biomass of Trichoderma atroviride TRS40 in solid state fermentation (SSF) cultures. The culture media overgrown with mycelium together conidia preserved by fluidised bed drying at various temperatures (50°C, 60°C and 70°C) the received biopreparations stored for 12 months. In order determine suitability production biopreparations, influence preservation process storage time on their survivability was examined. three-component mixture proved...
One of the examples physical starch modifications is retention a suspension in water having temperature slightly lower than pasting (annealing). The aim this study was to investigate effect annealing process performed at various temperatures as first stage modification. annealed preparations were then esterified using acetic acid anhydride. Finally, and acetylated determined for their properties. before acetylation triggered changes properties modified preparations. It contributed higher...
Abstract Flavin-dependent monooxygenases (FMOs) are a valuable group of biocatalysts that can regioselectively introduce hydroxy for the targeted modification biologically active compounds. Here, we present fdeE, FMO from Herbaspirillum seropedicae SmR1 is part naringenin degradation pathway and towards wide range flavonoids—flavanones, flavones, isoflavones, flavonols. Bioinformatics biochemical analysis revealed high similarity between analyzed enzyme other F8H FMOs what might indicate...