A5014281537- Bacterial Infections and Vaccines
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Respiratory viral infections research
- Pneumonia and Respiratory Infections
- Bacterial Genetics and Biotechnology
University of Lausanne
2020-2023
Competence development in the human pathogen Streptococcus pneumoniae controls several features such as genetic transformation, biofilm formation, and virulence. Competent bacteria produce so-called “fratricins” CbpD that kill noncompetent siblings by cleaving peptidoglycan (PGN). is a choline-binding protein (CBP) binds to phosphorylcholine residues found on wall lipoteichoic acids (WTA LTA) together with PGN are major constituents of pneumococcal cell wall. pneumococci protected against...
CRISPR-Cas systems provide bacteria and archaea with adaptive immunity against viruses plasmids by the detection cleavage of invading foreign DNA. Modified versions this system can be exploited as a biotechnological tool for precise genome editing at targeted locus. Here, we developed replicative plasmid that carries CRISPR-Cas9 RNA-programmable counterselection in opportunistic human pathogen
Abstract CRISPR systems provide bacteria and archaea with adaptive immunity against viruses plasmids by detection cleavage of invading foreign DNA. Modified versions this system can be exploited as a biotechnological tool for precise genome editing at targeted locus. Here, we developed novel, replicative plasmid that carries the CRISPR-Cas9 RNA-programmable, counterselection in opportunistic human pathogen Streptococcus pneumoniae . Specifically, demonstrate an approach making targeted,...
Abstract Competence development in the human pathogen Streptococcus pneumoniae controls several features such as genetic transformation, biofilm formation and virulence. Competent bacteria produce so called ‘fratricins’ CbpD, that kill non-competent siblings by cleaving peptidoglycan (PGN). CbpD is a choline-binding protein (CBP) binds to phosphorylcholine residues found on wall- lipoteichoic acids (WTA LTA) together with PGN are major constituents of pneumococcal cell wall. pneumococci...