Article10 May 2017Open Access Transparent process High-throughput CRISPRi phenotyping identifies new essential genes in Streptococcus pneumoniae Xue Liu orcid.org/0000-0001-6485-1865 Molecular Genetics Group, Groningen Biomolecular Sciences and Biotechnology Institute, Centre for Synthetic Biology, University of Groningen, The Netherlands Center Infectious Disease Research, School Medicine, Tsinghua University, Beijing, China Search more papers by this author Clement Gallay...

Streptococcus pneumoniae becomes competent for genetic transformation when exposed to an autoinducer peptide known as competence-stimulating (CSP). This was originally described a quorum-sensing signal, enabling individual cells regulate competence in response population density. However, recent studies suggest that CSP may instead serve probe sensing environmental cues, such antibiotic stress or diffusion. Here, we show induction can be simultaneously influenced by cell density, external...

ABSTRACT The treatment of life-threatening infections due to carbapenem-resistant Acinetobacter baumannii has become a serious challenge for physicians worldwide. Often, only colistin shows in general good vitro activity against these strains, but its antibacterial efficacy comparison with the antibiotics most used clinical practice is not well known. We studied versus those imipenem, sulbactam, tobramycin, and rifampin an experimental pneumonia model immunocompetent mice. three strains A....

Streptococcus pneumoniae can acquire antibiotic resistance by activation of competence and subsequent DNA uptake. Here, we demonstrate that aztreonam (ATM) clavulanic acid (CLA) promote competence. We show both compounds induce cell chain formation targeting the d,d-carboxypeptidase PBP3. In support hypothesis promotes competence, an autolysin mutant (ΔlytB) is hypercompetent. Since initiated binding a small extracellular peptide (CSP) to membrane-anchored receptor (ComD), wondered whether...

Competence development in the human pathogen Streptococcus pneumoniae controls several features such as genetic transformation, biofilm formation, and virulence. Competent bacteria produce so-called “fratricins” CbpD that kill noncompetent siblings by cleaving peptidoglycan (PGN). is a choline-binding protein (CBP) binds to phosphorylcholine residues found on wall lipoteichoic acids (WTA LTA) together with PGN are major constituents of pneumococcal cell wall. pneumococci protected against...

The increase in erythromycin resistance among Streptococcus pyogenes isolates is a cause for concern. We analysed trends macrolide resistance, phenotypes, genotypes, determinants and transposons erythromycin-resistant S. collected from adults Barcelona hospital (1993–2008). Antibiotic susceptibility was studied by microdilution. Molecular typing performed PFGE, emm multilocus sequence (MLST). Macrolide genes those related to the Tn916 family of were detected PCR. Ninety-nine (18.3%) 541...

We aimed to obtain insights on the nature of a collection isolates presumptively identified as atypical Streptococcus pneumoniae recovered from invasive and non-invasive infections in Spain. One-hundred thirty-two were characterized by: optochin susceptibility ambient CO(2)-enriched atmosphere; bile solubility; PCR-based assays targeting pneumococcal genes lytA, ply, pspA, cpsA, Spn9802, aliB-like ORF2, specific 16S rRNA region; multilocus sequence analysis; antimicrobial susceptibility. By...

Streptococcus pneumoniae (pneumococcus) is an opportunistic pathogen that causes otitis media, sinusitis, pneumonia, meningitis and sepsis. The progression to this pathogenic lifestyle preceded by asymptomatic colonization of the nasopharynx. This associated with biofilm formation; competence pathway influences structure stability biofilms. However, molecules link formation are unknown. Here, we describe a new competence-induced gene, called briC, demonstrate its product promotes development...

ABSTRACT Antimicrobial efficacy in orthopedic device infections is diminished because of bacterial biofilms which express tolerance to antibiotics. Recently, the use high doses levofloxacin with rifampin has been recommended for staphylococcal infections. In present study, we evaluated at 50 mg/kg/day and 100 (mimicking usual human 500 mg/day 750 1,000 mg/day, respectively) compared it that linezolid, cloxacillin, vancomycin, a rat tissue cage model experimental foreign-body infection by...

This study aimed to compare the antibiotic susceptibilities, serotypes and genotypes of pneumococci causing pneumonia or acute exacerbations chronic obstructive pulmonary disease (AECOPD) in patients with COPD. A total 611 collected from 487 COPD (n = 255, 94 bacteraemic pneumonia) AECOPD episodes 356), 2001 2008, were analysed. Antibiotic susceptibility was studied by microdilution. Serotypes (PCR Quellung) (PFGE multilocus sequence typing) determined. Pneumococci isolated significantly...

Adult invasive pneumococcal disease (IPD) occurs mainly in the elderly and patients with co-morbidities. Little is known about clinical characteristics, serotypes genotypes causing IPD healthy adults.We studied 745 culture-proven cases of adult aged 18-64 years (1996-2010). Patients were included two groups: 1.) adults co-morbidities, 2.) adults, who had no prior or coincident diagnosis a chronic immunosuppressive underlying disease. Microbiological studies serotyping genotyping.Of episodes,...

Capsular switching allows pre-existing clones of Streptococcus pneumoniae expressing vaccine serotypes to escape the vaccine-induced immunity by acquisition capsular genes from pneumococci a non-vaccine serotype. Here, we have analysed clonal composition 492 clinical isolates serotype 11A causing invasive disease in Spain (2000–2012), and their ability evade host immune response. Antibiograms, serotyping molecular typing were performed. The restriction profiles pbp2x, pbp1a pbp2b also...

Pneumolysin is a major virulence factor of Streptococcus pneumoniae that plays key role in interaction with the host during invasive disease. How pneumolysin influences these dynamics between and pathogen early phase central nervous system infection pneumococcal meningitis remains unclear. Using whole-animal vivo dual RNA sequencing (RNA-seq) approach, we identify pneumolysin-specific transcriptional responses both S. zebrafish (Danio rerio) meningitis. By functional enrichment analysis,...

ABSTRACT Biofilm formation by nontypeable (NT) Haemophilus influenzae remains a controversial topic. Nevertheless, biofilm-like structures have been observed in the middle-ear mucosa of experimental chinchilla models otitis media (OM). To date, there no studies biofilm large collections clinical isolates. This study aimed to investigate initial adhesion solid surface and NT H. comparing isolates from healthy carriers, those with noninvasive respiratory disease, invasive disease. We used 352...

As with most medicines historically, clinicians prescribing tildrakizumab have relied on information derived from registration studies undertaken in a prospective controlled clinical trial setting. More recently, clinicians, policymakers, and commissioners increasingly rely real-world data to inform both policy practice.A retrospective study was at four specialist dermatology departments the United Kingdom. All adult patients treated for moderate-to-severe plaque psoriasis were included,...

Abstract The distribution of interstitial cells Cajal (ICC) and neurotransmission were investigated in lower oesophageal sphincter (LES) circular muscle strips from Sprague–Dawley (SD) rats, Ws/Ws mutant rats their wild‐type (+/+) siblings. Intramuscular c‐Kit‐positive cells, confirmed to be ICC‐IM by electron microscopy, observed throughout both layers SD +/+ rats. In contrast, c‐Kit‐positive, ultrastructurally typical absent Ws/Ws. LES showed increased spontaneous contractile activity....

Antibiotic resistance in Streptococcus pneumoniae has increased worldwide by the spread of a few clones. Fluoroquinolone occurs mainly alteration their intracellular targets, type II DNA topoisomerases, which is acquired either point mutation or recombination. Increase fluoroquinolone-resistance may depend on balance between antibiotic consumption and cost that imposes to bacterial fitness. In addition, pneumococcal prophages could play an important role. Prophage induction fluoroquinolones...

Little is known about the sensitivity of BinaxNOW pneumococcal urinary antigen (PUA) test for adult pneumonia caused by different serotypes. In this study, we aimed to analyze trends in PUA over a 15-year period (2001 2015) and its total, analyzed 1,096 isolates from adults with who had performed at onset episode. Three periods were analyzed: 2001 2005 (early use seven-valent conjugate vaccine [early PCV7]), 2006 2010 (late PCV7), 2011 2015 PCV13). The varied 76.4% (95% confidence interval...

MEPS Marine Ecology Progress Series Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsTheme Sections 296:219-228 (2005) - doi:10.3354/meps296219 Life cycles and growth rates of two morphotypes Cystodytes (Ascidiacea) in western Mediterranean Susanna López-Legentil1,2,*, Markus Ruchty3, Arnau Domenech1, Xavier Turon1 1Department Animal Biology (Invertebrates), Faculty Biology, University Barcelona, 645,...

In Spain, rates of ciprofloxacin resistance in pneumococci were low during the last decade (2.6% 2002 and 2.3% 2006). 2012, rate remained at 2.3%, equivalent to 83 3,621 isolates. Of resistant isolates, 15 showed a level (MIC 4 8 μg/ml) 68 high 16 128 resistance. Thirteen low-level-resistant isolates had single changes ParC, one ParE change, did not present any mutations. High-level-resistant GyrA plus additional ParC and/or changes: 51, 15, 2 2, 3, or mutations, respectively. Although 24...

Pneumococci are an important cause of acute exacerbations in patients with chronic obstructive pulmonary disease (COPD). In the last decade, pneumococcal population has changed, mainly due to introduction 7-valent conjugate vaccine (PCV7). We analysed antimicrobial susceptibility (microdilution), serotype (PCR) and genotype (PFGE/multilocus sequence typing) pneumococci causing during period 2009–12. Results were compared two previously published historic periods (2001–04 2005–08). A total...