A5052496100- Zebrafish Biomedical Research Applications
- interferon and immune responses
- Cellular transport and secretion
- Autophagy in Disease and Therapy
- Developmental Biology and Gene Regulation
- RNA regulation and disease
- Invertebrate Immune Response Mechanisms
- Hippo pathway signaling and YAP/TAZ
- Animal Genetics and Reproduction
- Aquaculture disease management and microbiota
- Silk-based biomaterials and applications
- Ubiquitin and proteasome pathways
- Protein Tyrosine Phosphatases
- Estrogen and related hormone effects
- Physiological and biochemical adaptations
- Skin and Cellular Biology Research
- Turtle Biology and Conservation
- Neurobiology and Insect Physiology Research
- Pluripotent Stem Cells Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Fish biology, ecology, and behavior
- Biochemical and Structural Characterization
- Histone Deacetylase Inhibitors Research
- Signaling Pathways in Disease
- MicroRNA in disease regulation
University of Padua
2011-2020
Polytechnic University
2015
Lizards are amniotes regenerating the tail but not limb, and no information on their different gene expression is available.Transcriptomes of limb blastemas show differences in between two organs. In blastemal, snoRNAs Wnt signals appear up-regulated probably association with apical epidermal peg (AEP), an epithelial region that sustains regeneration absent limb. A balance pro-oncogenes tumor suppressors likely present blastema allowing a regulated proliferation. Small collagens, protease...
Abstract Glucocorticoids (GCs) play important roles in developmental and physiological processes through the transcriptional activity of their cognate receptor (Gr). Using CRISPR/Cas9 technology, we established a zebrafish null Gr mutant line compared its phenotypes with wild type partially silenced gr ( s357 / ). Homozygous −/− larvae are morphologically inconspicuous and, contrast to GR knockout mice, viable adulthood, although reduced fitness early life survival. Mutants fertile, but...
In zebrafish, ovulated oocytes contain both maternal cortisol and the mRNA for glucocorticoid receptor (gr), which is spread as granular structures throughout ooplasm. At 0.2 hpf, this transcript relocated in blastodisc area partitioned among blastomeres. 6–8 it replaced by zygotic transcript. We used morpholinos to block translation of gr transcripts, a missplicing morpholino post-transcriptionally alone. Only knockdown produced an increase apoptosis subsequent craniofacial caudal...
AMBRA1 is a positive regulator of the BECN1-dependent program autophagy recently identified in mouse. In this study, we cloned full-length cDNAs ambra1a and ambra1b zebrafish paralogous genes. As mouse, both Ambra1 proteins contain characteristic WD40 repeat region. The transcripts genes are present as maternal RNAs eggs display gradual decline until 8 hpf, being replaced by zygotic mRNAs from 12 hpf onwards. After 24 mainly localized head, suggesting possible role brain development. To...
The essential role of autophagy in muscle homeostasis has been clearly demonstrated by phenotype analysis mice with muscle-specific inactivation genes encoding autophagy-related proteins. Ambra1 is a key component the Beclin 1 complex and, zebrafish, it encoded two paralogous genes, ambra1a and ambra1b, both required for normal embryogenesis larval development. In this study we focused on function Ambra1, positive regulator autophagic process, during skeletal development means morpholino...
Glucocorticoids (GCs) modulate many cellular processes through the binding of glucocorticoid receptor (GR) to specific responsive elements located upstream transcription starting site or within an intron GC target genes. Here we describe a transgenic fish line harboring construct with nine GC-responsive (GREs) reporter (EGFP) coding sequence. Transgenic exhibit strong fluorescence in known organs. Moreover, its enhanced sensitivity allowed discovery novel tissue compartments, such as fin,...
The tough corneous layer in the carapace and plastron of hard-shelled turtles derives from accumulation keratin-associated beta-proteins (KAbetaPs, formerly called beta-keratins) while these proteins are believed to be absent soft-shelled turtles. Our bioinformatics molecular study has instead shown that epidermis turtle Apalone spinifera expresses like or even higher amount than Pseudemys nelsoni. analysis a cDNAs library allowed identification characterization three alpha-keratins type I...
Background/Aims: Probiotic strains have been recognized to exert important roles in many biological systems, including immune response, growth, development and reproduction. However, date, no studies focused either on the relation among probiotics maternal factors or probiotics' ability qualitatively and/or quantitatively modulate transcripts. Methods: In this study, effects of Lactobacillusrhamnosus administered parental fish control involved autophagic, apoptotic dorsalizing processes...
The EPG5 protein is a RAB7A effector involved in fusion specificity between autophagosomes and late endosomes or lysosomes during macroautophagy/autophagy. Mutations the human gene cause rare severe multisystem disorder called Vici syndrome. In this work, we show that zebrafish epg5-/- mutants from both heterozygous incrossed homozygous matings are viable can develop to age of sexual maturity without conspicuous defects external appearance. agreement with dysfunctional autophagy syndrome,...
Abstract Numerous bacteria are frequently observed in the superficial corneocytes forming corneous layer of soft‐shelled turtle Apalona spinifera . The resistance to bacterial penetration through living epidermis this suggests presence an antimicrobial barrier, possibly derived from anti‐microbial peptides epidermis. Four beta‐defensin‐like peptides, named As‐BD‐1 4, have been characterized skin tissues using molecular and bioinformatics methods. precursor contain beta‐defensin motif with...
In zebrafish, two paralogous genes, activating molecule in beclin-1 (BECN1)-regulated autophagy