A5052021490- Aquaculture Nutrition and Growth
- Reproductive biology and impacts on aquatic species
- Reproductive Biology and Fertility
- Aquaculture disease management and microbiota
- Microplastics and Plastic Pollution
- Sperm and Testicular Function
- Effects and risks of endocrine disrupting chemicals
- Aquatic life and conservation
- Spectroscopy Techniques in Biomedical and Chemical Research
- Insect Utilization and Effects
- Fish Biology and Ecology Studies
- Ovarian function and disorders
- Animal and Plant Science Education
- Animal Genetics and Reproduction
- Fish Ecology and Management Studies
- Physiological and biochemical adaptations
- Gut microbiota and health
- Identification and Quantification in Food
- Turtle Biology and Conservation
- Marine and fisheries research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Marine Biology and Environmental Chemistry
- Genetic diversity and population structure
- Cannabis and Cannabinoid Research
- Invertebrate Immune Response Mechanisms
Marche Polytechnic University
2015-2024
Istituto Nazionale Biostrutture e Biosistemi
2010-2024
Polytechnic University
2015
Elettra-Sincrotrone Trieste S.C.p.A.
2011
The widespread use of plastics determines the inevitable human exposure to its by-products, including microplastics (MPs), which enter organism mainly by ingestion, inhalation, and dermal contact. Once internalised, MPs may pass across cell membranes translocate different body sites, triggering specific cellular mechanisms. Hence, potential health impairment caused internalisation accumulation is prime concern, as confirmed numerous studies reporting evident toxic effects in various animal...
In fish, like other organisms, the lack of balance between production reactive oxygen species (ROS) and antioxidant defense system (so-called oxidative stress) can cause DNA hydroxylation, protein denaturation, lipid peroxidation, apoptosis, ultimately cell damage. To improve capability, different approaches such as administration synthetic antioxidants were practiced. During past years, dietary have been suggested a promising way increasing activity in fish shellfish species. Among them,...
Abstract The microbiome plays an important role in lipid metabolism but how the introduction of probiotic communities affects host is poorly understood. Using a multidisciplinary approach we addressed this knowledge gap using zebrafish model by coupling high-throughput sequencing with biochemical, molecular and morphological analysis to evaluate changes intestine. Analysis bacterial 16S libraries revealed that Lactobacillus rhamnosus was able modulate gut larvae, elevating abundance...
Abstract Bisphenol A (BPA) is one of the commonest Endocrine Disruptor Compounds worldwide. It interferes with vertebrate reproduction, possibly by inducing deregulation epigenetic mechanisms. To determine its effects on female reproductive physiology and investigate whether changes in expression levels genes related to reproduction are caused histone modifications, BPA concentrations consistent environmental exposure were administered zebrafish for three weeks. Effects oocyte growth...
Abstract The gut microbiota regulates metabolic pathways that modulate the physiological state of hunger or satiety. Nutrients in stimulate release several appetite modulators acting at central and peripheral levels to mediate glucose metabolism. After an eight-day exposure zebrafish larvae probiotic Lactobacillus rhamnosus , high-throughput sequence analysis evidenced ability microbial composition gastrointestinal tract. These changes were associated with a down-regulation up-regulation...
The Mediterranean Sea is particularly endangered by microplastics (MPs), polymers, and additives. These contaminants can be ingested fishes and, hence, translocate into tissues. We aimed to quantify MPs, polyethylene terephthalate (PET), polycarbonate (PC), bisphenol A (BPA), p-phthalic acid (PTA) in the edible muscles of swordfish (Xiphias gladius) bluefin tuna (Thunnus thynnus) caught Sea. MPs were extracted from characterized stereomicroscopy Raman microspectroscopy, while polymers (PET...
It is well known established that reproduction sensitive to the state of energy reserves, and there a balance between homeostasis fertility. In this view, study examined effects probiotic Lactobacillus rhamnosus , as feed additive, on zebrafish ( Danio rerio ) fecundity. Ten days treatment modulate gene expression neuropeptide hormones metabolic signals, such kiss1 kiss2 leptin both at CNS level peripheral level. The increase in fecundity brought about by was demonstrated higher number...
Most organisms modulate their reproductive activity responding to day length by the nocturnal release of melatonin pineal gland. This hormone is also responsible for synchronizing reproduction with specific external environment stimuli in order optimize success. The aim this study was establish effect on zebrafish reproduction. Adult females were daily exposed, via water, two different doses (100 nM and 1 µM) melatonin. Melatonin led an increase Gonado Somatic Index (GSI) associated eggs...
In this study, the effects of probiotic administration on zebrafish Danio rerio intestinal innate immunity and hepatic stress were evaluated. Zebrafish adults treated for 10 days with Lactobacillus rhamnosus IMC 501(®). To assess at molecular level, mRNA levels genes involved in immune system, response, oxidative stress, apoptosis quantified by real-time polymerase chain reaction. An increase biomarkers related to responses was observed tissue from probiotic-treated fish compared control...
Abstract Glucocorticoids (GCs) play important roles in developmental and physiological processes through the transcriptional activity of their cognate receptor (Gr). Using CRISPR/Cas9 technology, we established a zebrafish null Gr mutant line compared its phenotypes with wild type partially silenced gr ( s357 / ). Homozygous −/− larvae are morphologically inconspicuous and, contrast to GR knockout mice, viable adulthood, although reduced fitness early life survival. Mutants fertile, but...
The xenoestrogen bisphenol A (BPA) is a widespread plasticizer detectable within several ecosystems. BPA considered metabolic disruptor, affecting different organs; however, little known about its mechanism of action in the liver, which it triggers triglyceride accumulation. Adult zebrafish (Danio rerio) exposed to developed hepatosteatosis, was associated with an increase liver levels obesogenic endocannabinoids 2-arachidonoylglycerol and anandamide concomitant decrease...
Due to minimal environmental impact, compared most conventional feed commodities, insects deserve a growing attention as candidate ingredients for aquafeeds. This study tested, the first time during zebrafish larval rearing, effects of an increasing replacement (0%–25%–50%) fish meal by black soldier fly (BSF) full-fat prepupae meal. All diets were formulated be isonitrogenous and isolipidic. A multidisciplinary approach, including biometrics, histology, gas chromatography–mass spectrometry,...
Abstract Black Soldier Fly (BSF) meal is considered as an alternative, emerging and sustainable ingredient for aquafeed production. However, results on fish physiological responses are still fragmentary often controversial, while no studies available behavior in response to these new diets. The present work represents the first comprehensive multidisciplinary study aimed investigate zebrafish behavioural BSF-based Five experimental diets characterized by increasing inclusion levels (0, 25,...