A5039998343- Regulation of Appetite and Obesity
- Adipose Tissue and Metabolism
- Biochemical Analysis and Sensing Techniques
- Adipokines, Inflammation, and Metabolic Diseases
- Aquaculture Nutrition and Growth
- Neuropeptides and Animal Physiology
- Pancreatic function and diabetes
- Reproductive biology and impacts on aquatic species
- Hypothalamic control of reproductive hormones
- Growth Hormone and Insulin-like Growth Factors
- Neuroendocrine regulation and behavior
- Physiological and biochemical adaptations
- Diet, Metabolism, and Disease
- Cancer-related molecular mechanisms research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Diabetes Treatment and Management
- Aquaculture disease management and microbiota
- Reproductive Physiology in Livestock
- Reproductive Biology and Fertility
- Calpain Protease Function and Regulation
- Autophagy in Disease and Therapy
- Gut microbiota and health
- Animal Genetics and Reproduction
- Zebrafish Biomedical Research Applications
- Dietary Effects on Health
University of Saskatchewan
2016-2025
College of Western Idaho
2022
Western University of Health Sciences
2015
York University
2008-2012
University of British Columbia
2004-2009
Keele University
2008-2009
University of Alberta
2004-2005
In this study, we examined (i) the preprandial, postprandial and starvation-induced changes in preproghrelin mRNA expression serum ghrelin levels, (ii) effects of intracerebroventricular intraperitoneal administration on food intake goldfish. Slot blot analysis revealed a significant decrease hypothalamus (1 3 h after feeding) gut (3 feeding). A similar levels was also detected. fish that were unfed at regular feeding time, hypothalamic remained unchanged, while decreased time. Starvation...
Abstract The gut microbiota regulates metabolic pathways that modulate the physiological state of hunger or satiety. Nutrients in stimulate release several appetite modulators acting at central and peripheral levels to mediate glucose metabolism. After an eight-day exposure zebrafish larvae probiotic Lactobacillus rhamnosus , high-throughput sequence analysis evidenced ability microbial composition gastrointestinal tract. These changes were associated with a down-regulation up-regulation...
Nesfatin-1 is a recently discovered anorexigen encoded in the precursor peptide, nucleobindin-2 (NUCB2) mammals. To date, nesfatin-1 has not been described any non-mammalian species, although some information available sequenced genomes of several species. Our objective was to characterize fish.In present study, we employed molecular, immunohistochemical, and physiological studies structure, distribution, appetite regulatory effects vertebrate. A very high conservation NUCB2 sequences,...
Nesfatin-1 is a recently discovered anorexigen, and we first reported nesfatin-like immunoreactivity in the pancreatic β-cells. The aim of this study was to characterize effects nesfatin-1 on whole-body energy homeostasis, insulin secretion, glycemia. vivo continuous peripheral delivery using osmotic minipumps food intake substrate partitioning were examined ad libitum-fed male Fischer 344 rats. glucose-stimulated secretion (GSIS) isolated islets. L6 skeletal muscle cells rat adipocytes used...
Nesfatin-1 is a recently discovered multifunctional metabolic hormone abundantly expressed in the pancreatic islets. The main objective of this study to characterize direct effects nesfatin-1 on insulin secretion vitro using MIN6 cells and islets isolated from C57BL/6 mice. We also examined expression precursor protein, nucleobindin 2 (NUCB2) mRNA, immunoreactivity (ir) normal mice with streptozotocin-induced type 1 diabetes diet-induced obese (DIO) diabetes. stimulated glucose-induced...
Nesfatin-1 is a novel anorectic peptide encoded in the precursor protein nucleobindin-2 (NUCB2). We recently reported presence and appetite suppressing effects of nesfatin-1 goldfish. has been co-localised with ghrelin stomach rats. Whether influences other regulatory peptides goldfish remains unclear. The main objectives present study were to investigate whether co-localises goldfish, test exogenous endogenous ghrelin, cholecystokinin (CCK) orexin A (OXA). found co-localisation...
Nesfatin-1 is an anorexigen in goldfish. In the present study, we provide novel data indicating presence and regulatory effects of nesfatin-1 on hypothalamo-pituitary-ovarian (HPO) axis Nucleobindin-2 (NUCB2)/nesfatin-1-like immunoreactive (ir) cells are hypothalamus pituitary, suggesting a hypophysiotropic role for nesfatin-1. NUCB2/nesfatin-1-like ir colocalize gonadotropin-releasing hormone (GnRH) nucleus lateralis tuberis posterioris anterior goldfish hypothalamus. The with GnRH these...
In the present study, we explored whether dietary lipid content influences gut microbiome in adult zebrafish. Diets containing three different levels (high [HFD], medium [MFD], and low [LFD]) were administered with or without supplementation of Lactobacillus rhamnosus (P) to zebrafish order explore how may influence microbiome. Dietary shifted structure. The addition L. diets, induced transcriptional reduction orexigenic genes, upregulation anorexigenic decrease genes involved cholesterol...
We studied the in vitro and vivo effects of octanoylated goldfish ghrelin peptides (gGRL-19 gGRL-12) on luteinizing hormone (LH) growth (GH) release goldfish. gGRL-19 gGRL-12 at picomolar doses stimulated LH GH from dispersed pituitary cells perifusion static incubation. Incubation for 2 h with 10 nM 1 or increased LH-beta mRNA expression, whereas only expression. Somatostatin-14 abolished stimulatory culture. The secretagogue receptor antagonist d-Lys(3)-GHRP-6 inhibited ghrelin-induced...
Nesfatin-1, the N-terminal fragment of nucleobindin 2 (NUCB2), is an 82 amino-acid peptide that inhibits food intake and exerts weight-reducing effects. Nesfatin-1 has been proposed as a potential anti-obesity peptide. However, studies to date have mainly focused on acute satiety effects centrally administered nesfatin-1. The main objective our was characterize long-term/chronic peripheral administration nesfatin-1 whole-body energy balance metabolic partitioning in male Fischer 344 rats....