A5005137719- Potato Plant Research
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant Pathogens and Resistance
- Plant Stress Responses and Tolerance
- Soybean genetics and cultivation
- Plant Disease Resistance and Genetics
- Photosynthetic Processes and Mechanisms
- RNA Interference and Gene Delivery
- Entomopathogenic Microorganisms in Pest Control
- Plant-Microbe Interactions and Immunity
- Nematode management and characterization studies
- Plant Reproductive Biology
- Plant responses to water stress
- Microbial Metabolites in Food Biotechnology
- CRISPR and Genetic Engineering
- Lipid metabolism and biosynthesis
- Salivary Gland Tumors Diagnosis and Treatment
- Ear and Head Tumors
- Legume Nitrogen Fixing Symbiosis
- Advanced biosensing and bioanalysis techniques
- Plant Virus Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Antimicrobial Peptides and Activities
- Microbial Community Ecology and Physiology
Soochow University
2018-2024
Chenzhou First People's Hospital
2024
Second Affiliated Hospital of Soochow University
2023-2024
Sichuan University of Science and Engineering
2023-2024
Yunnan Arts University
2024
Nanjing Agricultural University
2019-2023
Huazhong Agricultural University
2008-2023
Tianjin Medical University
2019-2023
Tianjin Medical University Eye Hospital
2019-2023
Southwest University
2013-2022
DNA replication divides the circular Escherichia coli chromosome into equal arms (replichores). Visualization of pairwise combinations multiple genetic loci reveals that two replichores occupy separate nucleoid halves, with origin between; positions on each replichore recapitulate map. Sequential replication–segregation regenerates <left–right> structure by sequentially layering newly replicated to specific inner and outer edges developing sister nucleoids. Replication fork-dependent...
Summary Several eukaryotic Heme‐associated proteins (HAPs) have been reported to bind specifically DNA fragments containing CCAAT ‐box; however, the physiological functions and direct targets of these HAP in plants remain unclear. In this study, we showed that At 5A as a transcription factor interacted with motif vivo , AtXTH21 one target 5A, was involved freezing stress resistance. The AtHAP5A overexpressing were more tolerant, whereas loss‐of‐function mutant sensitive than wild‐type...
Cadmium (Cd) is harmful to plant growth and can be easily transferred from soil plants. Plant cell wall plays important role in preventing Cd entering cells. Salicylic acid (SA) mediated defense response increases resistance heavy metals. In this study, all tomato seedlings were pre-treated with 100 μM SA for 3 d, then used analyze the of regulating stress. The results showed that exogenous significantly reduced accumulation plants changed distribution. By analyzing composition, it was found...
Drought is one of major environmental determinants plant growth and productivity. Alfalfa (Medicago sativa) a legume perennial forage crop native to the arid semi-arid environment, which an ideal candidate study biochemical molecular mechanisms conferring drought resistance in plants. In this study, stress responses two alfalfa varieties, Longdong Algonquin, were comparatively assayed at physiological, morphological transcriptional levels. Under control condition, drought-tolerant with...
Summary The resurrection plant Craterostigma plantagineum possesses an extraordinary capacity to survive long‐term desiccation. To enhance our understanding of this phenomenon, complementary transcriptome, soluble proteome and targeted metabolite profiling was carried out on leaves collected from different stages during a dehydration rehydration cycle. A total 7348 contigs, 611 proteins 39 metabolites were differentially abundant across the sampling points. Dynamic changes in transcript,...
Antimicrobial peptides are important components of the host innate immune system, forming first line defense against infectious microorganisms. Among them, liver-expressed antimicrobial (LEAPs) a family that widely exist in vertebrates. LEAPs include two types, named LEAP-1 and LEAP-2, many teleost fish have or more LEAP-2s. In this study, LEAP-2C from rainbow trout grass carp were discovered, both which composed 3 exons 2 introns. The antibacterial functions multiple systematically compared...
The p38 signaling pathway is activated in response to cell stress and induces production of proinflammatory cytokines. P38α phosphorylated by MKK3 MKK6 turn phosphorylates a number substrates, including MAPKAP kinase 2 (MK2). We have determined the crystal structure unphosphorylated p38α-MK2 heterodimer. C-terminal regulatory domain MK2 binds docking groove p38α, ATP-binding sites both kinases are at heterodimer interface. conformation suggests an extra mechanism addition regulation p38α...
Summary Reducing sugar ( RS ) accumulation in cold‐stored potato tubers, known as cold‐induced sweetening CIS ), is a crucial factor causing unacceptable colour changes and acrylamide formation of fried products. The activity vacuolar invertase (Stvac INV 1) proved important for the process, inhibitors are speculated to play roles post‐translational regulation Stvac 1 activity. In our previous research, two putative (StInvInh2A StInvInh2B) were implied be involved . Here, we further reported...
Slowing down cold-induced sweetening (CIS) of potato (Solanum tuberosum) tubers is economic importance for the industry to ensure high-quality products. The conversion sucrose reducing sugars by acid invertase StvacINV1 thought be critical CIS. Identification specific inhibitor StInvInh2B and α- β-subunits interacting protein SUCROSE NONFERMENTING1-RELATED PROTEIN KINASE from wild species Solanum berthaultii (SbSnRK1) has led speculation that activity may regulated via a posttranslational...
Abstract Protein therapeutics targeting intracellular machineries hold profound potential for disease treatment, and hence robust cytosolic protein delivery technologies are imperatively demanded. Inspired by the super‐negatively charged, nucleotide‐enriched structure of nucleic acids, adenylated pro‐proteins (A‐proteins) with dramatically enhanced negative surface charges have been engineered first time via facile green synthesis. Then, thymidine‐modified polyethyleneimine is developed,...
The accumulation of reducing sugars in cold-stored tubers, known as cold-induced sweetening (CIS), negatively affects potato processing quality. starch to sugar interconversion pathways that are altered CIS tubers have been elucidated, but the mechanism regulates them remains largely unknown. This study identified a CBF/DREB transcription factor (StTINY3) enhances resistance by both activating biosynthesis and repressing hydrolysis sucrose detached tubers. Silencing StTINY3 CIS-resistant...
The plant homeodomain (PHD) finger is a Cys4HisCys3-type zinc which promotes protein-protein interactions and binds to the cis-acting elements in promoter regions of target genes. In Medicago truncatula, five PHD homologues with full-length sequence were identified. However, detailed function genes was not fully addressed.In this study, we characterized MtPHD6 during responses drought stress. highly induced by Ectopic expression Arabidopsis enhanced tolerance osmotic stresses. transgenic...
Sweet potato (Ipomoea batatas L.) is a nutritious food crop and, based on the high starch content of its storage root, potential bioethanol feedstock. Enhancing nutritional value and quantity roots are important goals sweet breeding programs aimed at developing improved varieties for direct consumption, processing, industrial uses. However, lines challenging due to genetic complexity this plant lack genome information. Short sequence repeat (SSR) markers powerful molecular tools tracking...
Abstract Potato invertase inhibitor ( StInvInh2 ) positively regulates cold-induced sweetening (CIS) resistance by inhibiting the activity of vacuolar invertase. The distinct expression patterns have been thoroughly characterized in different potato genotypes, but related CIS ability has not characterized. understanding regulatory mechanisms that control transcription is unclear. In this study, we identified an ERF‐VII factor, StRAP2.3 , directly to modulate resistance. Acting as a...
Drought stress restricts the growth, distribution and productivity of alfalfa (Medicago sativa L.). In order to study response differences cultivars drought stress, we previously carried out physiological molecular comparative analysis on two varieties with contrasting resistance (relatively drought-tolerant Longdong drought-sensitive Algonquin). However, in proteomic factors still need be further studied. Therefore, TMT-based quantitative was performed using leaf tissues identify uncover...
The modulation of the activity enzymes associated with carbohydrate metabolism is important for potato cold‐induced sweetening (CIS). A novel RING finger gene SbRFP1 was cloned and its expression found to be cold‐inducible in tubers CIS‐resistant genotypes. Transformation potatoes confirmed role inhibiting β‐amylase invertase activity, which consequently slowed down starch sucrose degradation accumulation reducing sugars cold stored tubers. These findings strongly suggest that may function...