A5100619619- Mycobacterium research and diagnosis
- Helicobacter pylori-related gastroenterology studies
- Biochemical and Molecular Research
- Tuberculosis Research and Epidemiology
- Adenosine and Purinergic Signaling
- Enzyme Structure and Function
- Antibiotic Resistance in Bacteria
- Pneumocystis jirovecii pneumonia detection and treatment
- Veterinary medicine and infectious diseases
- Cancer therapeutics and mechanisms
- Peptidase Inhibition and Analysis
- Enzyme function and inhibition
- Magnetic confinement fusion research
- Bioactive Compounds and Antitumor Agents
- Enterobacteriaceae and Cronobacter Research
- Leprosy Research and Treatment
- Viral gastroenteritis research and epidemiology
- Toxin Mechanisms and Immunotoxins
- Enzyme Production and Characterization
- Calcium signaling and nucleotide metabolism
- Virus-based gene therapy research
- Amino Acid Enzymes and Metabolism
- Animal Virus Infections Studies
- Fungal and yeast genetics research
- Microbial infections and disease research
National Institute of Infectious Diseases
2016-2025
Research Institute of Disinfectology Rospotrebnadzor
2022
National Institutes of Health
1994-2013
The University of Texas MD Anderson Cancer Center
2011
Kyoto University
2000-2005
Kawasaki Heavy Industries (Japan)
2002
Kyoto Bunkyo University
2000-2001
Kagoshima University
2001
Niigata University
1995
National Institute of Allergy and Infectious Diseases
1990-1992
NAD kinase was purified to homogeneity from Escherichia coli MG1655. The enzyme a hexamer consisting of 30 kDa subunits and utilized ATP or other nucleoside triphosphates as phosphoryl donors for the phosphorylation NAD, most efficiently at pH 7.5 60 °C. could not use inorganic polyphosphates designated ATP–NAD kinase. N‐terminal amino‐acid sequence encoded by yfjB , which had been deposited gene unknown function in E. whole genomic DNA database. cloned expressed BL21(DE3)pLysS. product...
Abstract Mycoplasma pneumoniae is a bacterial human pathogen that causes primary atypical pneumonia. M. motility and infectivity are mediated by the immunodominant proteins P1 P40/P90, which form transmembrane adhesion complex. Here we report structure of P1, determined X-ray crystallography cryo-electron microscopy, P40/P90. Contrary to what had been suggested, binding site for sialic acid was found in P40/P90 not P1. Genetic clinical variability concentrates on N-terminal domain surfaces...
ATP-NAD kinase phosphorylates NAD to produce NADP by using ATP, whereas ATP-NADH both and NADH. Three homologues, namely, (Utr1p), (Pos5p) function-unknown Yel041wp (Yef1p), are found in the yeast Saccharomyces cerevisiae. In this study, Yef1p was identified as an kinase. The activity of Utr1p also confirmed. Thus, three homologues were biochemically kinases. phenotypic analysis single, double triple mutants, which unexpectedly be viable, for UTR1, YEF1 POS5 demonstrated critical...
A novel subclass B3 metallo-β-lactamase (MBL), SMB-1, recently identified from a Serratia marcescens clinical isolate, showed higher hydrolytic activity against wide range of β-lactams than did the other MBLs, i.e., BJP-1 and FEZ-1, environmental bacteria. To identify mechanism underlying differences in substrate specificity among we determined structure using 1.6-Å diffraction data. Consequently, found that SMB-1 reserves space active site to accommodate β-lactam, even with bulky R1 side...
Helicobacter species infecting the stomachs of dogs and cats are potentially pathogenic have been isolated from patients with gastric diseases. In present study conducted in Japan, among nine strains that we cats, NHP19-003T a dog, NHP19-012T NHP21-005T were identified to be most closely related heilmannii ASB1T based on 16S rRNA comparison (98.7-99.2% similarity H. ASB1T). However, none their whole genomes showed more than average nucleotide identity (ANI) threshold value (95-96%) any...
ABSTRACT A gram-negative bacterium, Sphingomonas sp. strain A1, isolated as a producer of alginate lyase, has characteristic cell envelope structure and forms mouth-like pit on its surface. The is produced only when the cells have to incorporate assimilate alginate. An uptake-deficient mutant was derived from A1. One open reading frame, algS (1,089 bp), exhibiting homology bacterial ATP-binding domain an ABC transporter, cloned fragment complementing mutation. followed by two frames, algM1...
UTR1 of the yeast Saccharomyces cerevisiae was cloned from genomic DNA by polymerase chain reaction and expressed in Escherichia coli. Characterization purified UTR1p revealed that is a NAD kinase consisting six identical subunits with molecular mass 60 kDa. specifically phosphorylated presence ATP, dATP, or CTP as phosphoryl donors, most active at pH 8.0, 30 degrees C. Km values for ATP were determined to be 0.50 mM 0.60 mM, respectively.
The 5'-terminal palindrome of the ADV-G strain Aleutian mink disease parvovirus (ADV) was molecularly cloned and sequenced. A full-length molecular clone ADV-G, denoted pXVB, then constructed. When this transfected into cell cultures, infectious ADV could be rescued. Virus derived from pXVB nonpathogenic for adult mink, as is parent strain.
Street rabies virus (SRV)-infected T-lymphocyte-deficient (nude) mice, in contrast to euthymic did not develop hindlimb paralysis prior death. To document the role of T lymphocytes virus-associated paralysis, 10(8) spleen cells from normal immunocompetent mice were transferred nude and recipient challenged with SRV. One hundred percent reconstituted developed died. Depletion donor suspension transfer abrogated development but prevent deaths animals. Mice receiving virus-immune become...
Helicobacter cinaedi colonizes the colons of human and animals can cause colitis, cellulitis, sepsis in humans, with infections immunocompromised patients being increasingly recognized. However, methods for analyzing molecular epidemiology H. are not yet established. A genotyping method involving multilocus sequence typing (MLST) was developed used to analyze 50 isolates from Japanese hospitals addition 6 reference strains. Pulsed-field gel electrophoresis (PFGE) results were also compared...
Asparaginase was purified from Helicobacter pylori 26695 and its pathophysiological role explored. The Km value of asparagine 9.75 ± 1.81 μM at pH 7.0, the optimum range broad around a neutral pH. H. asparaginase converted extracellular to aspartate. cells were unable take up directly. Instead, aspartate produced by action transported into cells, where it partially β-alanine. exhibited striking cytotoxic activity against histiocytic lymphoma cell line U937 via deprivation. live significantly...
By using strand-specific in situ hybridization and immunohistochemistry, evidence for replication of the Aleutian mink disease parvovirus was observed cells resembling macrophages follicular dendritic at 10 days after infection but only 60 days. Sequestration larger numbers noted both
Forty-six Helicobacter cinaedi isolates from the same hospital were analyzed by multilocus sequence typing. Most H. exhibited clonal complex 9 and mainly isolated immunocompromised patients in ward. Three fennelliae obtained ward pulsed-field gel electrophoresis patterns. All resistant to clarithromycin ciprofloxacin. must be carefully monitored prevent nosocomial infection.
Metallo-β-lactamases (MBLs) confer resistance to carbapenems, and their increasing global prevalence is a growing clinical concern. To elucidate the mechanisms by which these enzymes recognize hydrolyze we solved 1.4 1.6 Å crystal structures of SMB-1 (Serratia metallo-β-lactamase 1), subclass B3 MBL, bound hydrolyzed carbapenems (doripenem, meropenem, imipenem). In structures, interacts mainly with carbapenem core structure via elements in active site, including zinc ion (Zn-2), Q157[113]...
NAD kinase phosphorylates NAD+ to form NADP+ and is strictly specific NAD+, whereas NADH both NADH, thereby showing relaxed substrate specificity. Based on their primary tertiary structures, the difference in specificities between kinases was proposed be caused by one aligned residue: Gly or polar amino acid (Gln Thr) five a charged (Arg) two kinases. The substitution of Arg with specificity (i.e. converted kinases). acids also specificity, hydrophobic did not show similar result. In...
Quinolinic acid phosphoribosyltransferase (QAPRTase, EC 2.4.2.19) is a key enzyme in the de novo pathway of nicotinamide adenine dinucleotide (NAD) biosynthesis and target for development new anti-tuberculosis drugs. QAPRTase catalyzes synthesis nicotinic mononucleotide from quinolinic (QA) 5-phosphoribosyl-1-pyrophosphate (PRPP) through phosphoribosyl transfer reaction followed by decarboxylation. The crystal structure Mycobacterium tuberculosis H37Rv (MtQAPRTase) has been determined;...
Mycobacterium smegmatis represents one model for studying the biology of its pathogenic relative tuberculosis. The structural characterization a M. tuberculosis ortholog protein can serve as valid tool development molecules active against target. In this context, we report biochemical and phosphoribosylpyrophosphate synthetase (PrsA), PrsA, unique enzyme responsible synthesis (PRPP). PRPP is key metabolite involved in several biosynthetic pathways including those histidine, tryptophan,...