A5026105791- Cancer Cells and Metastasis
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Cellular Mechanics and Interactions
- Cell Adhesion Molecules Research
- RNA modifications and cancer
- Fibroblast Growth Factor Research
- Wnt/β-catenin signaling in development and cancer
- Neonatal Respiratory Health Research
- Cancer-related gene regulation
- Virus-based gene therapy research
- Platelet Disorders and Treatments
- Force Microscopy Techniques and Applications
- Peptidase Inhibition and Analysis
- Mesenchymal stem cell research
- Cancer Research and Treatments
- Medical Imaging and Pathology Studies
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
Institute of Photonic Sciences
2020-2021
Institute for Research in Biomedicine
2019-2020
Hospital del Mar Research Institute
2014
Municipal Institute for Medical Research
2011-2012
Hospital Del Mar
2011-2012
Crosstalk between tumor and stromal cells in the microenvironment alter its properties ways that facilitate invasive behavior of cells. Here, we demonstrate cancer-associated fibroblasts (CAF) increase stiffness extracellular matrix (ECM) promote anisotropic fiber orientation, two mechanical signals generated through a Snail1/RhoA/αSMA-dependent mechanism sustains oriented cell migration invasiveness. Snail1-depleted CAF failed to acquire myofibroblastic traits response TGFβ, including RhoA...
Snail1 is a transcriptional repressor of E-cadherin that triggers epithelial–mesenchymal transition (EMT). Here, we report assisted interaction with the promoter typical mesenchymal gene, fibronectin (FN1), both in epithelial cells undergoing EMT and fibroblasts. Together Snail1, p65 subunit NF-κB PARP1 bound to FN1 promoter. We detected nuclear these proteins demonstrated requirement all three for transcription. Moreover, other genes involved cell movement mimic expression induced by or...
Myofibroblasts are a population of highly contractile fibroblasts that express and require the activity transcription factor Snail1. Cancer-associated (CAFs) correlate with low survival cancer patients when present in stroma primary tumors. Remarkably, presence myofibroblastic CAFs (which Snail1) creates mechanical properties tumor microenvironment support metastasis. However, therapeutic blockage fibroblast is double-edged sword, as normal activities often restrict cell invasion. We used...
Fibronectins are cell-secreted glycoproteins that modulate cell attachment, spreading, migration, morphology, differentiation and oncogenic transformation. Fibronectin expression is activated during EMT (epithelial-mesenchymal transition) a hallmark of mesenchymal cells. It shown in the present study transcription factor previously unrelated with EMT, TFCP2c/LSF/LBP-1c, was translocated to nucleus bound fibronectin promoter upon induction by Snail1. Consequently, interference...
Here, we describe a protocol to detect and visualize protein synthesis by click-chemistry-based immunofluorescence in patient-derived organoids (PDOs) vitro. The uses O-propargyl puromycin (OPP), an analog of that enters the acceptor site ribosomes is incorporated into nascent polypeptides. OPP can be detected click chemistry reaction combined with conventional antibody staining. We procedures for imaging intact 3D format or sections from paraffin blocks. For complete details on use...
ABSTRACT The leukocyte specific β 2 -integrin LFA-1, and its ligand ICAM-1 expressed on endothelial cells (ECs), are involved in the arrest, adhesion transendothelial migration of leukocytes. Although role mechanical forces LFA-1 activation is well established, impact major ICAM-1, has received less attention. Using a parallel-plate flow chamber combined with confocal super-resolution microscopy, we show that prolonged shear-flow induces global translocation ECs upstream direction....
<p>Supplementary Figure S1. TGF beta remodels the ECM generated by mesenchymal stem and 1BR3G cells in a Snail1-dependent manner. Supplementary S2. Cell Commitment is Modulated Fibroblast-derived Matrices Manner. S3. controls focal contacts S4. regulates N-cadherin intercellular S5. Alignment of MEFs bo-cultured with CAFs. Table Baseline Characteristics According to Snail1 Expression Stroma Overall Survival Analysis Patients Antibodies Used for Protein Analysis</p>
<p>Video S7. Ela-Myc cells on 3D-ECM generated by KO MEFs.</p>
<p>Video S2. MDA-MB231 cells on 3D-ECM generated by TGFβ-treated Control MEFs.</p>
<p>Video S8. Ela-Myc cells on 3D-ECM generated by TGFβ-treated KO MEFs.</p>
<p>Video S8. Ela-Myc cells on 3D-ECM generated by TGFβ-treated KO MEFs.</p>
<p>Video S6. Ela-Myc cells on 3D-ECM generated by TGFβ-treated Control MEFs.</p>
<p>Video S6. Ela-Myc cells on 3D-ECM generated by TGFβ-treated Control MEFs.</p>
<p>Video S1. MDA-MB231 cells on 3D-ECM generated by Control MEFs.</p>
<p>Video S6. Ela-Myc cells on 3D-ECM generated by TGFβ-treated Control MEFs.</p>
<p>Video S2. MDA-MB231 cells on 3D-ECM generated by TGFβ-treated Control MEFs.</p>