A5080778899- Immunotherapy and Immune Responses
- Cell Adhesion Molecules Research
- Glycosylation and Glycoproteins Research
- T-cell and B-cell Immunology
- Nanocluster Synthesis and Applications
- Immune Cell Function and Interaction
- Immunodeficiency and Autoimmune Disorders
- HIV Research and Treatment
- Phagocytosis and Immune Regulation
- Cellular Mechanics and Interactions
- Cancer Genomics and Diagnostics
- Advanced Fluorescence Microscopy Techniques
- Lipid Membrane Structure and Behavior
- Metal complexes synthesis and properties
- HIV/AIDS drug development and treatment
- Immune cells in cancer
- Plasmonic and Surface Plasmon Research
- Inflammatory mediators and NSAID effects
- Platelet Disorders and Treatments
- Cell death mechanisms and regulation
- RNA modifications and cancer
- Synthesis and Biological Evaluation
- Protease and Inhibitor Mechanisms
- Optical Coherence Tomography Applications
- Cell Image Analysis Techniques
Institute of Photonic Sciences
2014-2023
Institut Curie
2021-2022
Université Paris Sciences et Lettres
2021-2022
Centre National de la Recherche Scientifique
2021-2022
Sorbonne Université
2022
Radboud University Medical Center
2013
Nijmegen Institute for Scientist Practitioners in Addiction
2013
Radboud University Nijmegen
2012-2013
Radboud Institute for Molecular Life Sciences
2013
A single dose of the anti-CD20 monoclonal antibody rituximab induces a nearly complete B cell depletion in peripheral blood, but not secondary lymphoid organs. Modulation this remaining population due to treatment may contribute therapeutic effects rituximab. To assess vivo we used lymph nodes (LNs) collected during renal transplant surgery patients who had received 4 weeks earlier preparation for an ABO-incompatible transplantation. Rituximab resulted lower percentage naïve (IgD(+)CD27(-))...
Optical nanoantennas can efficiently confine light into nanoscopic hotspots, enabling single-molecule detection sensitivity at biological relevant conditions. This innovative approach to breach the diffraction limit offers a versatile platform investigate dynamics of individual biomolecules in living cell membranes and their partitioning cholesterol-dependent lipid nanodomains. Here, we present optical nanoantenna arrays with accessible surface hotspots study characteristic diffusion...
The immunoglobulin-like lectin receptor CD169 (Siglec-1) mediates the capture of HIV-1 by activated dendritic cells (DCs) through binding to sialylated ligands. These interactions result in a more efficient virus as compared resting DCs, although underlying mechanisms are poorly understood. Using combination super-resolution microscopy, single-particle tracking and biochemical perturbations we studied nanoscale organization Siglec-1 on DCs its impact viral trafficking single viral-containing...
Chromosome conformation in mammals is closely related to gene regulation. Within topologically associating domains, where genomic contacts are enriched, genes tend show correlated expression across tissues and conditions, suggesting domain-wide mechanisms coregulating multiple genes, such as enhancer sharing or local histone mark spreading. At the single-cell level, transcription occurs sporadic bursts, transcriptional coordination has been observed between proximal but how folding of...
Abstract Nanomaterials with very low atomicity deserve consideration as potential pharmacological agents owing to their small size and properties that can be precisely tuned minor modifications size. Here, it is shown silver clusters of three atoms (Ag 3 ‐AQCs)—developed by an ad hoc method—augment chromatin accessibility. This effect only occurs during DNA replication. Coadministration Ag ‐AQCs increases the cytotoxic DNA‐acting drugs on human lung carcinoma cells. In mice orthotopic...
Abstract Dendritic cells (DCs) are APCs essential in regulating the immune response. PGE2, produced during inflammation, has a pivotal role maturation of DCs and, therefore, is vital for The large variety biologic functions governed by PGE2 mediated its signaling through 4 distinct E-type prostanoid (EP) receptors. Immunogenic express EP2 and EP4, which mediate signaling. However, expression function EP receptors human tolerogenic (tol-DCs), present an inhibitory phenotype, have not yet, to...
Abstract MerTK is a transmembrane receptor tyrosine kinase (RTK) mainly expressed in dendritic cells (DCs) and macrophages where it plays an important role immunotolerance, but also activating oncogenic signalling pathways. Albeit exploited as clinical target cancer auto-immune disorders, the mechanisms that regulate its diverse functions are poorly understood. Here, we identified remarkably high pool of full nucleus human DCs. Nuclear translocation was ligand-dependent. Importantly, nuclear...
LFA-1 is a leukocyte specific β2 integrin that plays major role in regulating adhesion and migration of different immune cells. Recent data suggest on mature dendritic cells (mDCs) may function as chemokine-inducible anchor during homing DCs through the afferent lymphatics into lymph nodes, by transiently switching its molecular conformational state. However, mobility this process not yet known, despite importance lateral organization dynamics for LFA-1-mediated regulation broadly...
Chemokine stimulation of integrin α4β1-dependent T lymphocyte adhesion is a key step during trafficking. A central question regarding α4β1 function how its lateral mobility and organization influence affinity avidity following cell with chemokines and/or ligands. Using single particle tracking superresolution imaging approaches, we explored the spatial arrangement individual α4β1integrins on cells exposed to different activating stimuli. We show that CXCL12 leads rapid transient...
ABSTRACT The leukocyte specific β 2 -integrin LFA-1, and its ligand ICAM-1 expressed on endothelial cells (ECs), are involved in the arrest, adhesion transendothelial migration of leukocytes. Although role mechanical forces LFA-1 activation is well established, impact major ICAM-1, has received less attention. Using a parallel-plate flow chamber combined with confocal super-resolution microscopy, we show that prolonged shear-flow induces global translocation ECs upstream direction....
Introduction: A single dose of the anti-CD20 monoclonal antibody rituximab (RTX) induces a nearly complete B-cell depletion in peripheral blood. However, there remains residual population secondary lymphoid organs, such as spleen and lymph nodes. An intriguing question that to be answered is whether these remaining B cells are modified due binding by RTX. Methods: To mimic vivo situation, where exposed RTX but only partially depleted, obtained from organ donors were incubated vitro with low...
Evidence of the significance targeting chromatin compaction to increase therapeutic index chemotherapy is provided by Arturo López-Quintela, Fernando Domínguez, and co-workers in article number 1801317. Co-administration silver quantum clusters three atoms, which increases accessibility, raises efficacy DNA-binding drugs standard care-treatment most human tumors.