A5006643425- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Cancer Genomics and Diagnostics
- Gene expression and cancer classification
- Gene Regulatory Network Analysis
- RNA and protein synthesis mechanisms
- Nuclear materials and radiation effects
- Radioactive element chemistry and processing
- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Microfluidic and Bio-sensing Technologies
- Evolution and Genetic Dynamics
- High Temperature Alloys and Creep
- Bioinformatics and Genomic Networks
- Metal Alloys Wear and Properties
- Protein Structure and Dynamics
- Chemical Synthesis and Characterization
- DNA and Nucleic Acid Chemistry
- Bacterial Genetics and Biotechnology
- Metallurgical Processes and Thermodynamics
- Nuclear Materials and Properties
- Chromosomal and Genetic Variations
- Recycling and utilization of industrial and municipal waste in materials production
- Fatigue and fracture mechanics
- Surface Treatment and Coatings
Université Paris Sciences et Lettres
2016-2024
Sorbonne Université
2016-2024
Centre National de la Recherche Scientifique
2007-2024
Institut Curie
2016-2024
Société Française de Céramique
2019-2023
Weatherford College
2021
Dynamique du noyau
2019
Conditions Extrêmes et Matériaux Haute Température et Irradiation
2016-2017
Université d'Orléans
2016
National Institutes of Health
2012-2015
Synthesis of mRNA in eukaryotes involves the coordinated action many enzymatic processes, including initiation, elongation, splicing, and cleavage. Kinetic competition between these processes has been proposed to determine RNA fate, yet such coupling never observed vivo on single transcripts. In this study, we use dual-color single-molecule imaging living human cells construct a complete kinetic profile transcription splicing β-globin gene. We find that results multiple competing pathways...
Current models propose that boundaries of mammalian topologically associating domains (TADs) arise from the ability CTCF protein to stop extrusion chromatin loops by cohesin. While orientation motifs determines which pairs sites preferentially stabilize loops, molecular basis this polarity remains unclear. By combining ChIP-seq and single molecule live imaging we report positions cohesin, but does not control its overall binding dynamics on chromatin. Using an inducible complementation...
Our understanding of the physical principles organizing genome in nucleus is limited by lack tools to directly exert and measure forces on interphase chromosomes vivo probe their material nature. Here, we introduce an approach actively manipulate a genomic locus using controlled magnetic inside living human cell. We observed viscoelastic displacements over micrometers within minutes response near-piconewton forces, which are consistent with Rouse polymer model. results highlight fluidity...
Although physiological steroid levels are often pulsatile (ultradian), the genomic effects of this pulsatility poorly understood. By utilizing glucocorticoid receptor (GR) signaling as a model system, we uncovered striking spatiotemporal relationships between loading, lifetimes DNase I hypersensitivity sites (DHSs), long-range interactions, and gene regulation. We found that hormone-induced DHSs were enriched within ±50 kb GR-responsive genes displayed broad spectrum upon hormone withdrawal....
A significant part of eukaryotic noncoding DNA is viewed as the passive result mutational processes, such proliferation mobile elements. However, sequences lacking an immediate utility can nonetheless play a major role in long-term evolvability lineage, for instance by promoting genomic rearrangements. They could thus be subject to indirect selection. Yet, effect difficult isolate either vivo or vitro. Here, performing silico experimental evolution, we demonstrate that, under low mutation...
Gene promoters can be in various epigenetic states and undergo interactions with many molecules a highly transient, probabilistic combinatorial way, resulting complex global dynamics as observed experimentally. However, models of stochastic gene expression commonly consider promoter activity two-state on/off system. We here model single-gene that represent arbitrary prokaryotic or eukaryotic promoters, based on the interplay between factors, including energy-dependent remodeling enzymatic...
A new strategy for the confinement of iodine has been developed: it consists in incorporating iodate anions (IO3−) into hydroxyapatite (Ca10(PO4)6(OH)2, HA). The iodate-substituted HA was synthesized by precipitation, and characterized detail. Its thermal stability resistance to leaching were then evaluated, revealing promising properties view radioactive applications.
Abstract Background A number of studies have established that stochasticity in gene expression may play an important role many biological phenomena. This therefore calls for further investigations to identify the molecular mechanisms at stake, order understand and manipulate cell-to-cell variability. In this work, we explored played by chromatin dynamics regulation stochastic higher eukaryotic cells. Results For purpose, generated isogenic chicken-cell populations expressing a fluorescent...
Chromosome conformation in mammals is closely related to gene regulation. Within topologically associating domains, where genomic contacts are enriched, genes tend show correlated expression across tissues and conditions, suggesting domain-wide mechanisms coregulating multiple genes, such as enhancer sharing or local histone mark spreading. At the single-cell level, transcription occurs sporadic bursts, transcriptional coordination has been observed between proximal but how folding of...
Abstract The nucleus of a cell contains its genetic information in the form chromatin: polymers DNA and associated proteins. physical nature this polymer system is yet to be understood. Orthogonal experimental approaches probing chromosome structure, dynamics, mechanics typically suggest existence scaling relationships, leading widespread use scale-free, or fractal, models represent interphase chromosomes. However, currently, there no single model consistent with all reported exponents....
A cementation route was developed to prepare an iodate-bearing apatite phase, in view of the long-term conditioning radioactive iodine-129.
Iodate-substituted hydroxyapatites are novel phases of potential interest for the immobilisation radioactive iodine-129. However, local environment iodine in these is still unclear. Here, a combined experimental–computational strategy has been used to investigate mode incorporation iodates (IO3−) Ca–hydroxyapatite and Sr–hydroxyapatite lattices. On one hand, I K- L3-edge XANES (X-ray Absorption Near Edge Structure) spectra presented, showing that while structure around iodate similar two...
Summary Current models propose that boundaries of mammalian topologically associating domains (TADs) arise from the ability CTCF protein to stop extrusion chromatin loops by cohesin proteins (Merkenschlager & Nora, 2016; Fudenberg, Abdennur, Imakaev, Goloborodko, Mirny, 2017). While orientation motifs determines which pairs sites preferentially stabilize DNA (de Wit et al., 2015; Guo Rao 2014; Vietri Rudan 2015), molecular basis this polarity remains mysterious. Here we report positions...
This publication presents new advances in the field of refractories characterization. These laboratory methods that combine experiments and numerical analyses concern both thermomechanical thermochemical behaviour are illustrated through different examples: identification asymmetrical creep, determination elastic inelastic properties, measurements macroscopic deformation, phase transformations or corrosion kinetics. advanced techniques offer refractory community opportunities to improve...