A5007852177- Genomics and Chromatin Dynamics
- Cancer Genomics and Diagnostics
- RNA Research and Splicing
- Gene expression and cancer classification
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Particle Dynamics in Fluid Flows
- Microfluidic and Bio-sensing Technologies
- Statistical Methods and Bayesian Inference
- Photoacoustic and Ultrasonic Imaging
- Bacterial Infections and Vaccines
- Respiratory viral infections research
- Nanopore and Nanochannel Transport Studies
- Gene Regulatory Network Analysis
- Viral gastroenteritis research and epidemiology
- Molecular Biology Techniques and Applications
- T-cell and B-cell Immunology
- Optical Coherence Tomography Applications
- Immunotherapy and Immune Responses
- RNA and protein synthesis mechanisms
- Pneumonia and Respiratory Infections
- RNA Interference and Gene Delivery
- Virology and Viral Diseases
- Soil Geostatistics and Mapping
- Diffusion and Search Dynamics
Centre National de la Recherche Scientifique
2014-2022
Institut Curie
2021-2022
Université Paris Sciences et Lettres
2021-2022
Sorbonne Université
2017-2022
California Institute for Regenerative Medicine
2017-2020
Institut Pasteur
2017-2020
University of California, Berkeley
2017-2020
École Normale Supérieure - PSL
2014-2018
Institut de Biologie de l'École Normale Supérieure
2018
Centre de Gestion Scientifique
2017
Single-particle tracking (SPT) has become an important method to bridge biochemistry and cell biology since it allows direct observation of protein binding diffusion dynamics in live cells. However, accurately inferring information from SPT studies is challenging due biases both data analysis experimental design. To address bias, we introduce 'Spot-On', intuitive web-interface. Spot-On implements a kinetic modeling framework that accounts for known biases, including molecules moving...
Our understanding of the physical principles organizing genome in nucleus is limited by lack tools to directly exert and measure forces on interphase chromosomes vivo probe their material nature. Here, we introduce an approach actively manipulate a genomic locus using controlled magnetic inside living human cell. We observed viscoelastic displacements over micrometers within minutes response near-piconewton forces, which are consistent with Rouse polymer model. results highlight fluidity...
The life of a cell is governed by highly dynamical microscopic processes. Two notable examples are the diffusion membrane receptors and kinetics transcription factors governing rates gene expression. Different fluorescence imaging techniques have emerged to study molecular dynamics. Among them, correlation spectroscopy (FCS) single-particle tracking (SPT) proven be instrumental our understanding dynamics function. analysis SPT FCS an ongoing effort, despite decades work, much progress...
Significance Recent experiments have suggested that the energy released by a chemical reaction can propel its enzyme catalyst (for example, alkaline phosphatase). However, this topic remains controversial, partially due to indirect and ensemble nature of existing measurements. Here, we used recently developed single-molecule approaches monitor directly motions individual proteins in aqueous solution find single phosphatase enzymes do not diffuse faster under catalysis. Instead, demonstrate...
Abstract Despite improved surveillance capacities and WHO recommendations for subdistrict analysis, routine epidemic of acute bacterial meningitis in the African belt remains largely limited to district level. We evaluated appropriateness performance analyses at higher spatial resolution. used suspected data health centre (HC) resolution from Burkina Faso 14 districts spanning years 2004–2014 analysed them using spatio-temporal statistics generative models. An operational analysis compared...
Three-dimensional fluorescence microscopy based on Nyquist sampling of focal planes faces harsh trade-offs between acquisition time, light exposure, and signal-to-noise. We propose a 3D compressed sensing approach that uses temporal modulation the excitation intensity during axial stage sweeping can be adapted to microscopes without hardware modification. describe implementations lattice sheet microscope an epifluorescence microscope, show images beads biological samples reconstructed with...
Background: Bacterial meningitis causes a high burden of disease in the African belt, with regular seasonal hyperendemicity and sporadic short, but intense, localized epidemics during late dry season occurring at small spatial scale [i.e., below district level, individual health centers (HCs)]. In addition, epidemic waves larger geographic extent occur every 7–10 y. Although atmospheric dust load is thought to be an essential factor for hyperendemicity, its role remains hypothetic....
The pathophysiological mechanisms underlying the seasonal dynamic and epidemic occurrence of bacterial meningitis in African belt remain unknown. Regular seasonality (seasonal hyperendemicity) is observed for both meningococcal pneumococcal understanding this critical better prevention modelling. two principal hypotheses hyperendemicity during dry season imply (1) an increased risk invasive disease given asymptomatic carriage meningococci pneumococci; or (2) transmission these bacteria from...
Abstract Our understanding of the physical principles organizing genome in nucleus is limited by lack tools to directly exert and measure forces on interphase chromosomes vivo probe their material nature. Here, we present a novel approach actively manipulate genomic locus using controlled magnetic inside living human cell. We observe viscoelastic displacements over microns within minutes response near-picoNewton forces, which are well captured Rouse polymer model. results highlight fluidity...
ABSTRACT Single-particle tracking (SPT) has become an important method to bridge biochemistry and cell biology since it allows direct observation of protein binding diffusion dynamics in live cells. However, accurately inferring information from SPT studies is challenging due biases both data analysis experimental design. To address bias, we introduce “Spot-On”, intuitive web-interface. Spot-On implements a kinetic modeling framework that accounts for known biases, including molecules moving...
Meningococcal meningitis epidemics in the African belt consist of localised (LME) that reach attack proportions 1% within a few weeks. A meningococcal serogroup conjugate vaccine was introduced countries from 2010 on, but LME due to other serogroups continue occur. The mechanisms underlying are poorly understood, an association with respiratory pathogens has been hypothesised. We analysed national routine surveillance data high spatial resolution (health centre level) 13 districts Burkina...
Abstract Three-dimensional fluorescence microscopy based on Nyquist sampling of focal planes faces harsh trade-offs between acquisition time, light exposure, and signal-to-noise. We propose a 3D compressed sensing approach that uses temporal modulation the excitation intensity during axial stage sweeping can be adapted to microscopes without hardware modification. describe implementations lattice sheet microscope an epifluorescence microscope, show images beads biological samples...
Introduction This deposit contains the softwares used in paper: Spot-On: robust model-based analysis of single-particle tracking experiments. These scripts should enable a user to reproduce full simulations and analyses performed paper. Some are hosted elsewhere, we only reference them this description. In folder 170718_simSPT_simulations.zip : code generate confined motion simulation, including comprehensive README detailing all parameters for simulation. A more updated version software...