- Botany, Ecology, and Taxonomy Studies
- Plant Taxonomy and Phylogenetics
- Botanical Research and Chemistry
- Plant Diversity and Evolution
- Plant and Fungal Species Descriptions
- Botany and Plant Ecology Studies
- Plant and animal studies
- Plant Pathogens and Fungal Diseases
- African Botany and Ecology Studies
- Biological Control of Invasive Species
- Plant Ecology and Taxonomy Studies
- Mediterranean and Iberian flora and fauna
- Botanical Research and Applications
- Ecology and Conservation Studies
- Phytochemistry and Biological Activities
- Chemical synthesis and alkaloids
- Botanical Studies and Applications
- Plant tissue culture and regeneration
- Flowering Plant Growth and Cultivation
- Banana Cultivation and Research
- Fern and Epiphyte Biology
- Phytochemistry and Bioactive Compounds
- Advances in Cucurbitaceae Research
- Sesquiterpenes and Asteraceae Studies
- Seed and Plant Biochemistry
NOAA National Marine Fisheries Service
2024
NOAA National Marine Fisheries Service Alaska Fisheries Science Center
2024
Princeton University
2019-2023
MRC Laboratory of Molecular Biology
2023
Medical Research Council
2023
Planetary Science Institute
2010
University of California, Berkeley
2010
The University of Melbourne
2006
Reserve Bank of New Zealand
2004
Texas A&M University
1990-2002
Lifetime limited optical excitation lines of single nitrogen vacancy (NV) defect centers in diamond have been observed at liquid helium temperature. They display unprecedented spectral stability over many seconds and cycles. Spectral tuning the spin selective resonances was performed via application an external electric field (i.e. Stark shift). A rich variety shifts were including linear as well quadratic components. The ability to tune NV has potential applications quantum information processing.
Electron cryomicroscopy can, in principle, determine the structures of most biological molecules but is currently limited by access, specimen preparation difficulties, and cost. We describe a purpose-built instrument operating at 100 keV—including advances electron optics, detection, processing—that makes structure determination fast simple fraction current costs. The attains its theoretical performance limits, allowing atomic resolution imaging gold test specimens molecular hours....
The potential for interspecific genetic exchange was examined by monitoring flowering patterns, pollinator movement, and gene flow among experimental populations of the Texas gourd ( Cucurbita texana ) cultivars pepo. While patterns movement tended to maximize self‐pollination local exchange, effective pollen exceeded 1,300 m. This mediated solitary bee Xenoglossa strenua monitored tracking allozyme variants, produced hybrids in 5% progeny from plants. Interspecific occurred either direction...
A New World assemblage of tetraploid Chenopodium species (section , subsection Cellulata) includes two domesticates, C. quinoa Andean South America and nuttalliae Mexico. Both have been combined into a single the Mexican form has considered as possible derivative . The domesticates related, sympatric weed forms, berlandieri North hircinum Andes, were examined for variation in morphological biochemical characteristics also included program artificial hybridization. Results indicate that...
Abstract While most studies of biomolecular phase separation have focused on the condensed phase, relatively little is known about dilute phase. Theory suggests that stable complexes form in two-component phase-separating systems, impacting separation; however, these not been interrogated experimentally. We show such indeed exist, using an vitro reconstitution system a phase-separated organelle, algal pyrenoid, consisting purified proteins Rubisco and EPYC1. Applying fluorescence correlation...
Abstract One thousand hectares in the south-east sector of Banks Peninsula are being managed for protection and restoration native vegetation wildlife under a policy minimum interference. The probable pre-human cover (1000 yr B.P.), inferred from current evidence some historical records, was continuous forest, c. 55% it podo-carp/hardwood forest 45% Nothofagus forest. About 4% this old-growth survives. remaining area is diverse mosaic successional vegetation. Approximately 30% total...
Summary Rettig, J. H., Wilson, H. D. & Manhart, R.: Phylogeny of the Caryophyllales ‐ gene sequence data. Taxon 41: 201–209. 1992. ISSN 0040‐0262. Phylogenetic relationships among selected families were examined by analyses nucleotide sequences large subunit ribulose‐l,5‐bisphosphate carboxylase/oxygenase ( rbc L). Sequences from 12 Caryophyllalean using parsimony and bootstrap analysis. The analysis produced two minimal trees with 778 steps. Major clades supported are (1) Amaranthus...
Significance Recent experiments have suggested that the energy released by a chemical reaction can propel its enzyme catalyst (for example, alkaline phosphatase). However, this topic remains controversial, partially due to indirect and ensemble nature of existing measurements. Here, we used recently developed single-molecule approaches monitor directly motions individual proteins in aqueous solution find single phosphatase enzymes do not diffuse faster under catalysis. Instead, demonstrate...
Journal Article Gene Flow in Squash Species Get access Hugh D. Wilson Search for other works by this author on: Oxford Academic Google Scholar BioScience, Volume 40, Issue 6, June 1990, Pages 449–455, https://doi.org/10.2307/1311392 Published: 01 1990
Abstract Xylem fluid and cotyledon, stem, leaf tissue of eight watermelon [ Citrullus lanatus Thunb. (Matsum. Nakai)] cultivars differentially suspectible to races 0, 1, 2 Fusarium oxysporum f. sp. niveum , (E.F. Sm.) Synd. Hans., causal agent fusarium wilt, were assayed for general proteins specific enzymes using SDS IEF-PAGE starch gel electrophoresis (SGE). SGE detected no variant isozymes among the in six enzyme systems examined (GOT, MDH, PGI, IDH, PGM, PER); however, electrophoretic...
The taxonomic significance of those morphological characters that have been used to delimit Chenopodium sect. subsect. Cellulata has questioned. Species this alveolate-fruited group are therefore often not treated in regional manuals, especially covering the eastern United States. Alveolate- fruited species usually included with synonymy C. album s. lat. Leiosperma. validity was tested through two programs artificial hybridization. Results support circumscription as a unit distinct from...
The physical limit in determining the atomic structure of biological molecules is radiation damage. In electron cryomicroscopy, there have been numerous attempts to reduce effects damage by cooling specimen beyond liquid-nitrogen temperatures, yet all failed realize potential improvement for single-particle determination. We identified causes information loss at liquid-helium and overcome them using a combination nanoscale beam illumination gold support with 100 nm diameter holes. This...
Starch gel electrophoresis was employed to examine allozyme variation within and between the domesticated ornamental gourds (Cucurbita pepo var. ovifera) closely related, wild C. texana. Data on 12 loci representing six enzyme systems (GOT, IDH, MDH, PER, PGI, PGM) were analyzed for 17 texana populations from Texas, spontaneous beyond gourd cultivars, four edible cultivars. Populations of relatively homogeneous with low levels polymorphism mean heterozygosity, while diversity among much...
Quantitative data were collected for 24 commercial cultivars of Cucurbita pepo, four C. pepo collections from Mexico, six populations texana Texas, and three spontaneous texana-like plants Alabama, Arkansas, Illinois. Canonical variate prin- cipal component analyses revealed variation in the size shape seeds which was similar to recently documented patterns allozyme variation. Additionally, seed characters appears reflect effects human selection. Stepwise selection used select useful...
The potential for interspecific genetic exchange was examined by monitoring flowering patterns, pollinator movement, and gene flow among experimental populations of the Texas gourd (Cucurbita texana) cultivars Cucurbita pepo. While patterns movement tended to maximize self-pollination local exchange, effective pollen exceeded 1,300 m. This mediated solitary bee Xenoglossa strenua monitored tracking allozyme variants, produced hybrids in 5% progeny from plants. Interspecific occurred either...
The domesticated Andean grain chenopod, Chenopodium quinoa, is sympatric with closely related wild types that are classified as two species. Large-fruited (1.5-1.8 mm diam.), broad- leaved plants placed C. either subsp. milleanum or var. melanospermum. Plants smaller fruits (1.0-1.5 mm) and rhombic to tri-lobed leaves treated varieties of hircinum, a species originally described from the eastern plains South America. This alignment was evaluated by comparative analysis electrophoretic...
Twelve allozyme loci were examined in a complex of closely related diploid (2 n = 18) members the genus Chenopodium occurring western United States. These plants are generally treated taxonomically as belonging to C. atrovirens, desiccatum, hians, leptophyllum , and pratericola . Also included populations referable incognitum The enzymes allelic designations given follows: leucine aminopeptidase, Lap; phosphoglucoisomerase, Pgi‐1, Pgi‐2; glutamate‐oxaloacetate transaminase, Got‐1, Got‐2,...