A5103133447- Enzyme Catalysis and Immobilization
- Antimicrobial Peptides and Activities
- Biochemical and Structural Characterization
- Enzyme function and inhibition
- CRISPR and Genetic Engineering
- Drug Transport and Resistance Mechanisms
- Biofuel production and bioconversion
- Cancer therapeutics and mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Microbial Metabolic Engineering and Bioproduction
- Pluripotent Stem Cells Research
- RNA Interference and Gene Delivery
- Catalysis for Biomass Conversion
- Pharmacogenetics and Drug Metabolism
- Steroid Chemistry and Biochemistry
- Signaling Pathways in Disease
- Helicobacter pylori-related gastroenterology studies
- Viral gastroenteritis research and epidemiology
Chinese University of Hong Kong
2012-2023
University of Hong Kong
2023
East China University of Science and Technology
2020-2022
Advanced strategies to interconvert cell types provide promising avenues model cellular pathologies and develop therapies for neurological disorders. Yet, methods directly transdifferentiate somatic cells into multipotent induced neural stem (iNSCs) are slow inefficient, it is unclear whether pass through a pluripotent state with full epigenetic reset. We report iNSC reprogramming from embryonic aged mouse fibroblasts as well human blood using an engineered Sox17 (eSox17FNV). eSox17FNV...
Abstract Ac CHMO, a cyclohexanone monooxygenase from Acinetobacter calcoaceticus , is typical Type I Baeyer–Villiger (BVMO). We previously obtained the CHMO M6 mutant, which oxidizes omeprazole sulfide (OPS) to chiral sulfoxide drug esomeprazole. To further improve catalytic efficiency of focused mutagenesis strategy was adopted at intersections FAD‐binding domain, NADPH‐binding and α ‐helical domain based on structural characteristics CHMO. By using subsequent global evolution two key...
Baeyer-Villiger monooxygenases (BVMOs) are important biocatalysts for the enzymatic synthesis of chiral sulfoxides, including sulfoxide-type proton pump inhibitors treatment gastrointestinal diseases. However, native BVMOs not yet suitable practical application due to their unsatisfactory activity and thermostability. Although protein engineering approaches can help address these issues, few feasible high-throughput methods available such enzymes. Herein, a colorimetric detection method...
Human cathelicidin (LL‐37) is implicated in cancer development. In this study, we demonstrate that LL‐37 was substantially downregulated primary tumor tissues of 58 out 60 cases colon as revealed by immunohistochemistry. Functional characterization indicates reduced cell viability and induced apoptosis cultured cells evidenced increased phosphatidylserine externalization DNA fragmentation. The pro‐apoptotic action caspase‐independent neither caspase activation nor its could be blocked a...