A5054634762- Proteoglycans and glycosaminoglycans research
- Glycosylation and Glycoproteins Research
- Advanced Proteomics Techniques and Applications
- SARS-CoV-2 and COVID-19 Research
- Metabolomics and Mass Spectrometry Studies
- Fibroblast Growth Factor Research
- SARS-CoV-2 detection and testing
- Neuroscience and Neuropharmacology Research
- Carbohydrate Chemistry and Synthesis
- Liver physiology and pathology
- Medicinal Plants and Bioactive Compounds
- Mass Spectrometry Techniques and Applications
- Nanoplatforms for cancer theranostics
- Inhalation and Respiratory Drug Delivery
- Effects of Radiation Exposure
- Biotin and Related Studies
- Biosensors and Analytical Detection
- Amino Acid Enzymes and Metabolism
- Ultrasound and Hyperthermia Applications
- Infrared Thermography in Medicine
- Cystic Fibrosis Research Advances
- Asthma and respiratory diseases
- Erythrocyte Function and Pathophysiology
- Neonatal Respiratory Health Research
- COVID-19 Clinical Research Studies
University of Cambridge
2010-2023
United Nations Industrial Development Organization
2012
Addenbrooke's Hospital
2008
GlaxoSmithKline (United Kingdom)
2003-2008
University of Liverpool
1996-2001
Inserm
1991-1994
University of Buckingham
1994
Hôpital Renée Sabran
1991
Centre National de la Recherche Scientifique
1991
Bayer (France)
1991
We have demonstrated by affinity chromatography that hepatocyte growth factor/scatter factor (HGF/SF) binds strongly to dermatan sulfate (DS), with a similar ionic strength dependence previously seen heparan (HS). Analysis of binding kinetics on biosensor yields an equilibrium dissociation constant,<i>K</i> <sub>D</sub>, 19.7 nm. This corresponds 10–100-fold weaker interaction than HS, primarily due faster rate the complex. The smallest DS oligosaccharide significant for HGF/SF appears be...
We have determined the relationship between binding sites for acidic fibroblast growth factor (aFGF) and basic FGF (bFGF) in heparan sulfate (HS) prepared from a panel of mammary cell lines ability HS to activate aFGF bFGF DNA synthesis assays. The ka fell into three groups, whereas kd (0.0015-0.016 s-1) Kd (0.4-8.6 microM) formed continuum. possessed high affinity site (Kd 22-30 nM) with fast (320,000-550,000 M-1 s-1), termed "fast/high," lower 47-320 slower (35,000-150,000 "slow/low." Most...
Peroxisome proliferator-activated receptor gamma (PPARgamma) is a ligand-dependent transcription factor that belongs to the nuclear family plays critical role in adipocyte differentiation and lipid metabolism. Here we report for first time PPARgamma expressed human renal cortical collecting ducts (CCD), segments of nephor involved regulation sodium water homeostasis via action epithelial channel (ENaC). ENaC activity regulated by hormones aldosterone insulin, primarily through co-ordinate...
There is still a lack in the molecular comprehension of major depressive disorder (MDD) although this condition affects approximately 10% world population. Protein phosphorylation posttranslational modification that regulates one-third human proteins involved range cellular and biological processes such as signaling. Whereas phosphoproteome studies have been carried out extensively cancer research, few investigations psychiatric disorders. Here, we present comparative analysis postmortem...
Frontotemporal lobar degeneration (FTLD) is an umbrella term for a heterogeneous group of young-onset dementias uncertain prevalence and incidence worldwide. Atypical cases FTLD with fused in sarcoma inclusions (aFTLD-U) have been described recently, but their molecular characterization still due. Using shotgun mass spectrometry, we identified total 107 differentially expressed proteins the prefrontal cortex, cerebellum occipital lobe from aFTLD-U patients compared to controls. These are...
Establishing preclinical models is essential for novel drug discovery in schizophrenia. Most existing are characterized by abnormalities behavioral readouts, which informative, but do not necessarily translate to the symptoms of human disease. Therefore, there a necessity characterizing from molecular point view. Selective reaction monitoring (SRM) has already shown promise and clinical studies multiplex measurement diagnostic, prognostic treatment-related biomarkers. We have established an...
Hepatocyte growth factor/scatter factor (HGF/SF) is a heparan sulfate (HS)-binding and morphogen for mammary epithelial cells that produced by stromal fibroblasts. HS chains, purified as peptidoglycans from panel of cell lines representative the ductal (Huma 123), myoepithelial 109), fibroblast (Rama 27), malignant (MCF-7 ZR-75), were used in biosensor-based assay to identify classes HGF/SF-binding sites polysaccharide chains. At least three distinct binding identified. One site exhibits...
Mucin glycopeptides were prepared from the respiratory mucus of a non-secretor, chronic bronchitis patient with blood group O, Lea+b−. Oligosaccharides released by alkaline borohydride treatment and purified anionexchange chromatography, size-exclusion chromatography high-performance liquid on silica-bonded alkylamine column. Structural studies employed 500-MHz 1H-NMR spectroscopy fast atom bombardment-mass spectrometry. Twenty-six monosialyl oligosaccharides ranging in size di- to...
In order to ascertain whether or not the presence of glycosaminoglycans in sputa patients suffering from chronic bronchial disorders was related tracheobronchial infection, an electrophoretic procedure set up. The different acidic macromolecular components sputum, namely nucleic acids, glycosaminoglycans, and glycopeptides could be identified proteolyzed sputum using agarose electrophoresis before after action enzymes: nucleases, chondroitinases, hyaluronidase heparinase. This used analyze...
Human respiratory-mucin glycopeptides were isolated from the sputum of a patient suffering cystic fibrosis. They subjected to treatment with alkaline borohydride. Application ion-exchange chromatography afforded carbohydrate fractions containing sulphate. One these mixture sulphated oligosaccharides was subsequently submitted gel-filtration and h.p.l.c. Two oligosaccharide-alditols, A B, prepared; their structure determined by means 400 MHz 1H-n.m.r. spectroscopy fast-atom-bombardment m.s....
Glycerol and trehalose have been widely examined as protective agents in the cryopreservation of red blood cells (RBCs). However, effectiveness these reagents alone on cell viability is moderate. Here, addition salidroside attenuated oxidative damage sheep RBCs prior to post cryostorage. The supplementation media containing 10% glycerol improved RBC survival by approximately 61.1±4.8% vs 37.9±4.6%. A smaller effect was seen cryopreserved 300 mM where 7.6±0.3%. Furthermore, cold storage...
Nanomedicine is seen as a potential central player in the delivery of personalized medicine. Biocompatibility issues nanoparticles have largely been resolved over past decade. Despite their tremendous progress, less than 1% applied nanosystems can hit intended target location, such solid tumor, and this remains an obstacle to full ability with high translational value. Therefore, achieving immune-tolerable, blood-compatible, biofriendly unmet need. The success nanoformulations from bench...
Pluronic-polydopamine nanocarriers were used to evaluate the effect of particle size on immunocompatiblity and cell uptake in pancreatic cancer. The drug-loaded carriers exhibited enhanced antiproliferative effects this hard-to-treat
Mammalian orthologues of the Drosophila tribbles protein (Trb1, Trb2 and Trb3) are a recently described family signalling molecules that regulate gene expression by modulation kinase pathways. In present study, screen for mRNA species specifically regulated in vulnerable regions human atherosclerotic plaque demonstrated up-regulation both Trb1 Trb2, latter more than 8-fold. vitro experiments primary monocyte-derived macrophages showed was up-regulated treatment with oxidized LDL (low-density...
The studies of neuropsychiatric disorders would be facilitated by enhanced knowledge the dorsolateral prefrontal cortex (DLPFC) proteome. To construct a data set human DLPFC proteins, protein extracts were prepared from 12 postmortem brains focussing on region (Brodmann area 9) and analyzed using combined gel electrophoresis shotgun mass spectrometry approach, featuring data-independent label-free nanoflow liquid chromatography (nLC-MS(E)). detected mass/time features aligned annotated...
Electroconvulsive therapy (ECT) is a psychiatric treatment in which seizures are electrically induced patients. Prior to treatment, patients usually given short-acting anaesthetics and muscle relaxants avoid harm, e.g. musculoskeletal injury, during the convulsions. However, most molecular studies investigating mechanism of action ECT have not explored potential effects pre-treatment with anaesthetic and/ or relaxant.We carried out targeted proteome analysis using multiplex immunoassay...
Conjugated polymers are increasingly exploited for biomedical applications. In this work, we explored the optical characteristics of conjugated variable chemical structures at multiple levels relevant to biological interfacing, from fluorescence yield their influence on cellular membrane potential. We systematically compared performance polymer as cast thin films and nanoparticles stabilized with amphiphilic polyethylene glycol-poly lactic acid-co-glycolic acid (PEG-PLGA). assessed in both...
Abstract We present the first characterization of human occipital lobe (primary visual cortex) and cerebellum proteomes. Proteins were identified using a combination gel electrophoresis data‐independent nanoflow liquid chromatography mass spectrometry (nLC‐MS E ). The resulting data sets comprised 391 330 unique proteins in cerebellum, respectively, at least 75% analyzed samples with 297 found common. These have been associated previously conditions, such as neurological disorder,...
An isolated preparation of the guinea pig trachea was developed. The exposed serosally to Krebs-Henseleit solution and mucosally tidal airflow, designed mimic conditions in vivo. establishes stable layers airway surface liquid (ASL). Typical depth, transepithelial potential differences, sodium activity are 200 microns, -3 mV, 125 mM, respectively (approximate concn 170 mM). When air with a vapor pressure deficit (VPD), evaporation water occurs from ASL, ASL depth decreases, concentration...