A5067372265- Spaceflight effects on biology
- Space Exploration and Technology
- Infant Nutrition and Health
- Gut microbiota and health
- Breastfeeding Practices and Influences
- Planetary Science and Exploration
- Pediatric health and respiratory diseases
- Microbial Community Ecology and Physiology
- Medical and Biological Ozone Research
- Polar Research and Ecology
- Plant Pathogens and Fungal Diseases
- Indoor Air Quality and Microbial Exposure
- Mycotoxins in Agriculture and Food
- Space Science and Extraterrestrial Life
- Infection Control and Ventilation
- Neonatal Respiratory Health Research
- Diet and metabolism studies
- SARS-CoV-2 detection and testing
- Genomics and Phylogenetic Studies
- Salivary Gland Disorders and Functions
- Digestive system and related health
- Dental Implant Techniques and Outcomes
- High Altitude and Hypoxia
- Biosensors and Analytical Detection
- Epigenetics and DNA Methylation
Jet Propulsion Laboratory
2018-2024
ZIN Technologies (United States)
2020-2023
Creative Research Enterprises (United States)
2023
University of Bern
2023
Jackson and Tull (United States)
2023
Ente Ospedaliero Cantonale
2023
Seoul National University Hospital
2023
Università della Svizzera italiana
2023
California Institute of Technology
2022
Royal Victoria Eye and Ear Hospital
2021
ABSTRACT In the United States, 1 in 8 women will be diagnosed with breast cancer her lifetime. Along genetics, environment contributes to disease development, but what these exact environmental factors are remains unknown. We have previously shown that tissue is not sterile contains a diverse population of bacteria. thus believe host's local microbiome could modulating risk development. Using 16S rRNA amplicon sequencing, we show bacterial profiles differ between normal adjacent from and...
In recent years, a greater appreciation for the microbes inhabiting human body sites has emerged. female mammary gland, milk been shown to contain bacterial species, ostensibly reaching ducts from skin. We decided investigate whether there is microbiome within tissue. Using 16S rRNA sequencing and culture, we analyzed breast tissue 81 women with without cancer in Canada Ireland. A diverse population of bacteria was detected collected all around aged 18 90, not whom had history lactation. The...
Human milk is an important source of bacteria for the developing infant and has been shown to influence bacterial composition neonate, which in turn can affect disease risk later life. Very little known about what factors shape human microbiome. The goal present study was examine microbiota from a range women who delivered vaginally or by caesarean (C) section, gave birth males females, at term preterm.Milk collected 39 Caucasian Canadian women, microbial profiles were analyzed 16S ribosomal...
Abstract In this study, the potential effects of bacteria on efficacy frequently used chemotherapies was examined. Bacteria and cancer cell lines were examined in vitro vivo for changes killing mediated by chemotherapeutic agents. Of 30 drugs , 10 found to be significantly inhibited certain bacteria, while same improved six others. HPLC mass spectrometry analyses sample (gemcitabine, fludarabine, cladribine, CB1954) demonstrated modification drug chemical structure. The chemoresistance or...
The International Space Station (ISS) is a closed system inhabited by microorganisms originating from life support systems, cargo, and crew that are exposed to unique selective pressures such as microgravity. To date, mandatory microbial monitoring observational studies of spacecraft space stations have been conducted traditional culture methods, although it known many microbes cannot be cultured with standard techniques. fully appreciate the true number diversity survive in ISS, molecular...
Antimicrobial resistance (AMR) is a global health issue. In an effort to minimize this threat astronauts, who may be immunocompromised and thus at greater risk of infection from antimicrobial resistant pathogens, comprehensive study the ISS "resistome' was conducted. Using whole genome sequencing (WGS) disc diffusion antibiotic assays, 9 biosafety level 2 organisms isolated were assessed for their resistance. Molecular analysis AMR genes 24 surface samples collected during 3 different...
The International Space Station (ISS) is a complex built environment physically isolated from Earth. Assessing the interplay between microbial community of ISS and its crew important for preventing biomedical structural complications long term human spaceflight missions. In this study, we describe one crewmember's profile body swabs mouth, nose, ear, skin saliva that were collected at eight different time points pre-, during post-flight. Additionally, environmental surface samples habitable...
Human milk is an important source of bacteria for the developing infant and has been shown to influence bacterial composition neonatal gut, which in turn can affect disease risk later life. also nutrients, influencing but directly affecting host. While recent studies have emphasized adverse effects antibiotic therapy on microbiota, maternal chemotherapy not previously studied. Here we report drug administration microbiota metabolome human milk. Mature was collected every two weeks over a...
The International Space Station (ISS) is a uniquely enclosed environment that has been continuously occupied for the last two decades. Throughout its operation, protecting health of astronauts on-board high priority. human microbiome plays significant role in maintaining health, and disruptions have linked to various diseases. To evaluate effects spaceflight on microbiome, body swabs saliva samples were collected from four ISS consecutive expeditions. Astronaut analyzed using shotgun...
Abstract Background The International Space Station (ISS) is a unique and complex built environment with the ISS surface microbiome originating from crew cargo or life support recirculation in an almost entirely closed system. Microbial Tracking 1 (MT-1) project was first environmental study to report on metagenome profiles without using whole-genome amplification. surveyed microbial communities eight surfaces over 14-month period. 2 (MT-2) aimed continue work of MT-1, sampling additional...
Abstract Background Spaceflight impacts astronauts in many ways but little is known on how spaceflight affects the salivary microbiome and consequences of these changes astronaut health, such as viral reactivation. In order to understand this, was analyzed with 16S rRNA gene amplicon sequencing, saliva titers were quantitative polymerase chain reaction (qPCR) primers specific for Epstein-Barr virus (EBV), herpes simplex (HSV), varicella zoster (VZV) from 10 pre-flight, in-flight,...
Microbial diversity estimation involves extracting nucleic acids from intricate sample matrices. Preparing acid samples is time-consuming, necessitating effective cell lysis and obtaining pure, inhibitor-free purifications before further use. An automated system offers advantages for field deployment due to its ease of use quick autonomous results. This especially beneficial rapid measurement in situ microbial remote areas. Our study aimed assess Yellowstone hot springs using a...
Until relatively recently, the extent of microbiota presence in human breast was under-appreciated. A high-throughput sequencing study and culture-based studies have demonstrated extensive microbes milk, with their origin believed to be from skin, oral cavity via gut translocation. Since formula milk substitutes do not contain these bacteria, what benefits are denied infants? The addition probiotic bacteria some infant is meant provide benefits, but only one species dose high compared milk....
Abstract Breast cancer is the most diagnosed amongst women worldwide. We have previously shown that there a breast microbiota which differs between who and those are disease-free. To better understand local biochemical perturbations occurring with disease potential contribution of microbiome, lipid profiling was performed on non-tumor tissue collected from 19 healthy 42 cancer. Here we identified unique signatures two groups greater amounts lysophosphatidylcholines oxidized cholesteryl...
Here, we present the whole-genome sequences of two Fusarium oxysporum isolates cultured from infected Zinnia hybrida plants that were grown onboard International Space Station (ISS).
This is the first study to isolate and characterize F. oxysporum isolates from a built environment, as well one that has been exposed space. The characterization analysis of these two genomes may have important implications for medical, agricultural, food industries health crew who coinhabit ISS with strains.
The Spacecraft Assembly Facility (SAF) at the NASA's Jet Propulsion Laboratory is primary cleanroom facility used in construction of some planetary protection (PP)-sensitive missions developed by NASA, including Mars 2020 Perseverance Rover that launched July 2020. SAF floor samples (n=98) were collected, over a 6-month period 2016 prior to rover subsystems, better understand temporal and spatial distribution bacterial populations (total, viable, cultivable, spore) this unique...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus that causes disease 2019, is a primarily transmitted person to through inhalation of droplets or aerosols, laden with viral particles. However, as recent studies have shown, virions can remain infectious for up 72 h on surfaces, which lead transmission contact. Thus, comprehensive study was conducted determine efficiency protocols recover SARS-CoV-2 from surfaces in built environments. This end-to-end (E2E) showed...
Bacteria are able to adapt and survive in harsh changing environments through many mechanisms, with one of them being horizontal gene transfer (HGT). This process is the leading culprits spread antimicrobial resistance (AMR) within bacterial communities could pose a significant health threat astronauts if they fell ill, especially on long-duration space missions. In order better understand degree HGT activity that occur space, biosafety level-2, donor recipient bacteria were co-cultured...
Humans have been exploring space for almost 55 years but travel comes with many psychological and physiological changes that astronauts to adapt to, both during post flight missions. Now, the reality of such missions lasting years, maintaining proper health crew is a high priority. While conditions as nausea, bone loss, renal calculi depression recognized, approaches medical surgical care in considered, influence microbiota could be added significance astronaut health. probiotics long part...