A5074531579- Spaceflight effects on biology
- Space Exploration and Technology
- Indoor Air Quality and Microbial Exposure
- Polar Research and Ecology
- Gut microbiota and health
- Atmospheric chemistry and aerosols
- Research Data Management Practices
- Clostridium difficile and Clostridium perfringens research
- Air Quality Monitoring and Forecasting
- High Altitude and Hypoxia
- Surgical site infection prevention
- Microbial bioremediation and biosurfactants
- Medical and Biological Ozone Research
- Wound Healing and Treatments
- Classical Antiquity Studies
- Microbial Community Ecology and Physiology
- Space Science and Extraterrestrial Life
- Medieval Philosophy and Theology
- Biomedical and Engineering Education
- Biblical Studies and Interpretation
- Hydraulic Fracturing and Reservoir Analysis
- Bacterial Identification and Susceptibility Testing
- Protist diversity and phylogeny
- RNA Research and Splicing
- Catholicism and Religious Studies
Lawrence Livermore National Laboratory
2020-2023
Northern Illinois University
2022
University of Florida
2017-2020
Oklahoma State University
2012
The International Space Station (ISS) is a uniquely enclosed environment that has been continuously occupied for the last two decades. Throughout its operation, protecting health of astronauts on-board high priority. human microbiome plays significant role in maintaining health, and disruptions have linked to various diseases. To evaluate effects spaceflight on microbiome, body swabs saliva samples were collected from four ISS consecutive expeditions. Astronaut analyzed using shotgun...
Abstract Background The International Space Station (ISS) is a unique and complex built environment with the ISS surface microbiome originating from crew cargo or life support recirculation in an almost entirely closed system. Microbial Tracking 1 (MT-1) project was first environmental study to report on metagenome profiles without using whole-genome amplification. surveyed microbial communities eight surfaces over 14-month period. 2 (MT-2) aimed continue work of MT-1, sampling additional...
The human spaceflight environment is notable for the unique factor of microgravity, which exerts numerous physiologic effects on macroscopic organisms, but how this may affect single-celled microbes less clear. In an effort to understand microbial transcriptome responds spaceflight, model Gram-positive bacterium Bacillus subtilis was flown two separate missions International Space Station in experiments dubbed BRIC-21 and BRIC-23. Cells were grown late-exponential/early stationary phase,...
Abstract To explore how airborne microbial patterns change with height above the Earth’s surface, we flew NASA’s C-20A aircraft on two consecutive days in June 2018 along identical flight paths over US Sierra Nevada mountain range at four different altitudes ranging from 10,000 ft to 40,000 ft. Bioaerosols were analyzed by metagenomic DNA sequencing and traditional culturing methods characterize composition diversity of atmospheric samples compared experimental controls. The relative...
Past results have suggested that bacterial antibiotic susceptibility is altered during space flight. To test this notion, Bacillus subtilis cells were cultivated in matched hardware, medium, and environmental conditions either flight microgravity on the International Space Station, termed (FL) samples, or at Earth-normal gravity, ground control (GC) samples. The of FL GC samples was compared to 72 antibiotics growth-inhibitory compounds using Omnilog phenotype microarray (PM) system. Only 9...
Results from previous experiments indicated that the Gram-negative α-proteobacterium Serratia liquefaciens strain ATCC 27592 was capable of growth under low temperature (0 °C), pressure (0.7 kPa), and anoxic, CO2-dominated atmosphere-conditions intended to simulate near-subsurface environment Mars. To probe response its transcriptome this extreme environment, S. cultivated 4 different environmental simulations: 0 °C, 0.7 kPa, CO2 atmosphere (Condition A); ~101.3 B); ambient N2/O2 C); 30 D;...
Co-infections or secondary infections with SARS-CoV-2 have the potential to affect disease severity and morbidity. Additionally, influence of nasal microbiome on COVID-19 illness is not well understood. In this study, we analyzed 203 residual samples, originally submitted for testing, presence viral, bacterial, fungal pathogens non-pathogens using a comprehensive microarray technology, Lawrence Livermore Microbial Detection Array (LLMDA). Eighty-seven percent samples were nasopharyngeal 23%...
Abstract Battlefield injury management requires specialized care, and wound infection is a frequent complication. Challenges related to characterizing relevant pathogens further complicates treatment. Applying metagenomics wounds offers comprehensive path toward assessing microbial genomic fingerprints could indicate prognostic variables for future decision support tools. Wound specimens from combat-injured U.S. service members, obtained during surgical debridements before delayed closure,...
Although clinostats have long been used in space microbiology studies as ground-based analogs of spaceflight, few to date systematically compared -omics data from versus spaceflight. This study the transcriptomic response Gram-positive bacterium Bacillus subtilis flown with corresponding transcriptomes derived 2-D clinostat (High Aspect Ratio Vessel: HARV) experiments performed under same conditions bacterial strain, growth medium, temperature, and incubation time. High-quality total RNA...
Ensuring the validity of results from funded programs is a critical concern for agencies that sponsor biological research. In recent years, open science movement has sought to promote reproducibility by encouraging sharing not only finished manuscripts but also data and code supporting their findings. While these innovations have lent support third-party efforts replicate calculations underlying key in scientific literature, fields inquiry where privacy considerations or other sensitivities...