A5032142710- Circadian rhythm and melatonin
- Estrogen and related hormone effects
- Hormonal and reproductive studies
- Stress Responses and Cortisol
- Birth, Development, and Health
- Nuclear Receptors and Signaling
- Sperm and Testicular Function
- Adipose Tissue and Metabolism
- Neuroendocrine regulation and behavior
- Identification and Quantification in Food
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Nitric Oxide and Endothelin Effects
- Botanical Research and Chemistry
- Ruminant Nutrition and Digestive Physiology
- Neuropeptides and Animal Physiology
- Protein Hydrolysis and Bioactive Peptides
- Hypothalamic control of reproductive hormones
- Medicinal Plant Pharmacodynamics Research
- Coenzyme Q10 studies and effects
- Hormonal Regulation and Hypertension
- Phosphodiesterase function and regulation
- Genetic and Environmental Crop Studies
- Sexual function and dysfunction studies
- Dietary Effects on Health
- Genetics, Aging, and Longevity in Model Organisms
University of Novi Sad
2011-2023
In mammals, circadian clock regulates concentration of many reproductive hormones including testosterone. Previously, we characterized pattern transcription core genes in testosterone-producing Leydig cells. Here, the potential role luteinizing hormone receptor (LHR)-cAMP signaling synchronization cell's and rhythmic testosterone production were examined. Results showed that activation LHR-cAMP primary rat cell culture increased Star/STAR changed expression (upregulated Per1/PER1, Dec1/2,...
Alfalfa is an autotetraploid, allogamous and heterozygous forage legume, whose varieties are synthetic populations. Due to the complex nature of species, information about genetic diversity germplasm used in any alfalfa breeding program most beneficial. The five varieties, involved progeny tests at Institute Field Vegetable Crops, was characterized based on RAPD markers. A total 60 primers were screened, out which 17 selected for analysis diversity. 156 polymorphic bands generated, with 10.6...
The molecular mechanism of the aging-associated dysfunction Leydig cells (LCs) is complex and poorly understood. In this study, we analyzed contribution nitric oxide (NO) cGMP signaling to age-dependent decline in LC function. Significant (>50%) decreases serum, intratesticular, androgens aging rats (15-24 months) were accompanied by a proportional increase NO production, an up-regulation levels, expression soluble guanylyl cyclase-1B protein kinase G1 LCs. contrast, cAMP levels decreased...
Abstract Since mitochondria play an essential role in the testosterone biosynthesis, serve as power centers and are a source of oxidative stress, possible mitochondrial dysfunction could be connected with decreased activity Leydig cells lowered production during aging. Here we chronologically analyzed age-related alterations function correlated by progressive rise cGMP signaling respect to synthesis. To target cells, acute or long-term vivo ex treatments sildenafil (phosphodiesterase 5...
The stress-induced initiation of proapoptotic signaling in Leydig cells is relatively well defined, but the duration this and mechanism(s) involved opposing stress responses have not been addressed. In study, immobilization (IMO) was applied for 2 h daily, animals were euthanized immediately after first (IMO1), second (IMO2), 10th (IMO10) sessions. IMO1 IMO2 rats, serum corticosterone adrenaline elevated, whereas androgens mRNA transcription insulin-like factor-3 inhibited. Reduced oxygen...
This study was designed to systematically analyze and evaluate the effects of in vivo blockade α 1 -adrenergic receptors (α -ADRs) on stress-induced disturbance steroidogenic machinery Leydig cells. Parameters followed 1) enzymes/proteins, transcription factors, cAMP/testosterone production; 2) main hallmarks stress (epinephrine, glucocorticoids); 3) profiles ADRs oxidases with high affinity inactivate glucocorticoids. Results showed that sustained -ADRs prevented decrease...
The molecular mechanism of stress-associated reproductive dysfunction is complex and largely unknown. This study was designed to systematically analyze effects systemic in vivo blockade α1-adrenergic receptors (α1-ADRs) on stress-induced disturbance cAMP/cGMP signaling testosterone-producing Leydig cells using the following parameters (i) level circulating stress hormones, LH testosterone; (ii) main markers cell functionality (testosterone, Insl3, cAMP); (iii) expression cAMP (cAMP...
Abstract The involvement of the molecular clock in regulating cell physiological processes on a specific time scale is recognized concept, yet its impact optimizing androgen production Leydig cells has been unclear. This study aimed to confirm role REVERBA (NR1D1) gene controlling transcription key genes related steroid production. We investigated daily variations by collecting from rats at various times within 24‐h period. Chromatin immunoprecipitation showed time‐dependent pattern for...
Anabolic-androgenic steroids (AAS) are synthetic derivatives of testosterone predominantly taken as drugs abuse. Using in vivo treatment adult male rats we investigated the effects enanthate (TE) a wide abused AAS, on apoptosis Leydig cells. Increased and decreased luteinizing hormone levels serum intra-testicular content were found 2 10 weeks treated groups. Two TE-treatment mitochondrial membrane potential increased prevalence cell apoptosis. The incidence returned to control after but...