Climate changes are affecting the plant production, including sugar beet growing especially in southern and central parts of Europe. Modifying sowing harvesting dates one most often used adaptations cultivation. The aim this study was to assess interactions between planting date genotypes for different harvest with recommendation duration vegetation period specific hybrids order achieve best performance evaluate influence climatic factors on yield. Three-way analysis variance AMMI (Additive...

Abstract There are some reports that low doses of γ-irradiation could induce antioxidant activities in plant material, including soybean. Irradiation, required for the inactivation pathogens and induction mutations, may have adverse effects on sensorial, nutritional qualities. The different (100–200 Gy) properties soybean seeds was investigated. In this study, we report results obtained by analysis enzyme activities, reduced glutathione, malonyldialdehyde (MDA) hydroxyl (HO−) radical...

Alfalfa is an autotetraploid, allogamous and heterozygous forage legume, whose varieties are synthetic populations. Due to the complex nature of species, information about genetic diversity germplasm used in any alfalfa breeding program most beneficial. The five varieties, involved progeny tests at Institute Field Vegetable Crops, was characterized based on RAPD markers. A total 60 primers were screened, out which 17 selected for analysis diversity. 156 polymorphic bands generated, with 10.6...

Genetic diversity studies are important for the selection of parents with a greater combination capacity that, when crossed, increase chances obtaining superior genotypes. Thus, 26 polymorphic simple sequence repeat (SSR) primers were used to assess genetic 140 individual samples from 12 diploid sugar beet pollinators (pollen parents) and two cytoplasmic male sterile (cms) lines (seed parents). Eight originated three research centers in United States Department Agriculture, while four cms...

The objective of this work was to quantify the accumulation major seed storage protein subunits, β-conglycinin and glycinin, how they influence yield oil contents in high-protein soybean genotypes. relative subunits calculated by scanning SDS-PAGE gels using densitometry. content tested genotypes higher than control cultivar same maturity group. Several with improved unchanged or were developed as a result new breeding initiatives. This research confirmed that cultivars accumulate amounts...

The molecular diversity studies of alfalfa (Medicago sativa L.) germplasm could contribute to a more precise selection parental populations in many breeding programs. Sequence-related amplified polymorphism (SRAP) markers were used assess the genetic 110 individual plants from 13 selected cultivars, landraces, and natural Tunisia, Australia, Serbia, Kazakhstan. Ten polymorphic SRAP primer combinations generated 137 alleles with 0.90 information content. percentage bands per genotype ranged...

In marker assisted breeding programs, determination of genome polymorphism and development suitable molecular markers is the greatest importance. The aim this research was RAPD markers, which will enable quick cost efficient DNA analysis among closely related sugar beet genotypes. conducted on twelve genotypes from population Reactions with eight primers five primer mixtures resulted in stable reproducible bands all samples, 44 polymorphic 14 monomorphic loci, average 6.13 per primer....

This work describes the incidence and distribution of most important bean viruses in Serbia: Bean common mosaic virus (BCMV), necrosis (BCMNV), yellow (BYMV), Cucumber (CMV) Alfalfa (AMV). The viral isolates were characterized serologically biologically. BCMV was found largest number plants (30.53%), followed by BCMNV (2.67%), CMV (5.34%), AMV (3.41%), since BYMV not determined. Mixed infections several samples. RT-PCR method used to prove that tested belong BCMV, family Potyviridae strains...

Rubbery taproot disease (RTD) of sugar beet was observed in Serbia for the first time 1960s. The already described neighboring Bulgaria and Romania at but it associated with abiotic factors. In this study on RTD its main growing area Serbia, we provide evidence association between ‘Candidatus Phytoplasma solani’ (stolbur phytoplasma) infection occurrence typical symptomatology. ‘Ca. P. identified by PCR sequence analyses 16S ribosomal RNA, tuf, secY, stamp genes. contrast, causative agent...

Sugar beet is a significant industrial crop, often grown in the areas where summer drought can severely limit root yield and sugar content.In order to improve development of cultivars with increased tolerance it necessary understand plant response water stress at genomic level.Since recent research efforts have focused on molecular identify deficit inducible genes, aim this investigation was develop qRT-PCR methodology for quantification gene expression under conditions deficiency...

During culture of protoplasts in agarose droplets, permanent problem was bacterial infection. It assumed that the seeds are origin infection, so different sterilization methods were tested order to overcome this problem. Germination, infection and hypocotyls their growth examined. Based on these parameters, best result obtained with combined use 5% commercial bleach dry heating at 45?C.

Abstract The aim of investigation was to evaluate genetic variation and relationship among alfalfa populations their offspring, with minimal cost, by using DNA marker analysis. RAPD analysis performed on bulked samples five parental progenies: 20 F1 from reciprocal diallel crosses S1 self-pollination. Twenty primers generated 217 bands, ranging in size 300 6000 bp, the average number bands per primer 10.85 polymorphism information content 0.246. Percentage polymorphic loci, effective...

At the time of soybean seed sowing in field, a high soil moisture, low and air temperatures, crasts formation may occur, which can lead to slow germination, poor seedling establishment, some cases loss vigor. Due importance prevalence aim this study was determine quality viability different genotypes produced at three locations Vojvodina during 2009 2010. Eight varieties (Afrodita, Valjevka, Balkan, Novosadjanka, Ravnica, Ana, Vojvodjanka Venera) Vrbas, Senta Indjija 2010 were tested. Seed...

Sugar beet is the most important crop for sugar production in Europe. Wide genetic variability essential breeding programs. The aim of this study to evaluate main root traits and differences between monogerm multigerm genotypes from collection at Institute Field Vegetable Crops. following were analyzed: weight (g), dry matter content (%), head root/head ratio number cambial rings, length (cm) diameter (cm). Mean values two years per genotype standardized used analysis. Principal Component...

The progress of alfalfa breeding has been slow, most notably due to its complex genetic structure (autotetraploidy) and tetrasomic inheritance. Alfalfa programs are based on recurrent phenotypic selection with or without progeny testing. control major agronomic traits is determined by both additive gene action (accumulation frequency desirable alleles represented significant general combining ability (GCA) effects, nonadditive action, complementary interactions specific (SCA) effects. This...