A5051849500- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- Evolution and Genetic Dynamics
- Escherichia coli research studies
- Genomics and Chromatin Dynamics
- Microplastics and Plastic Pollution
- Advanced Fluorescence Microscopy Techniques
- Additive Manufacturing and 3D Printing Technologies
- Effects and risks of endocrine disrupting chemicals
- Cell Image Analysis Techniques
Technical University of Denmark
2021-2024
Novo Nordisk Foundation
2021-2024
Foundation Center
2021
Novozymes (Denmark)
2021
University of Oxford
2016
Abstract Poly(ethylene terephthalate) (PET) is the world’s most abundant polyester plastic, and its ongoing accumulation in nature causing a global environmental problem. Currently, main recycling processes utilize thermomechanical or chemical means, resulting deterioration of mechanical properties PET. Consequently, polluting de novo synthesis remains preferred, creating need for more efficient bio-sustainable ways to hydrolyze polymer. Recently, PETase enzyme from bacterium Ideonella...
The evolution of microorganisms often involves changes unclear relevance, such as transient phenotypes and sequential development multiple adaptive mutations in hotspot genes. Previously, we showed that ageing colonies an E. coli mutant unable to produce cAMP when grown on maltose, accumulated the crp gene (encoding a global transcription factor) genes involved pyrimidine metabolism cmk; combined both cmk enabled fermentation maltose (which usually requires cAMP-mediated Crp activation for...
Abstract Gene expression toxicity is an important biological phenomenon and a major bottleneck in biotechnology. Escherichia coli BL21(DE3) the most popular choice for recombinant protein production, various derivatives have been evolved or engineered to facilitate improved yield tolerance toxic genes. However, previous efforts evolve BL21, such as Walker strains C41 C43, resulted only decreased strength of T7 system. This reveals little about mechanisms at play constitutes marginal progress...
Here we report that mutations in the topA gene compensate for loss of cAMP, showing interplay between Crp and supercoiling status promoters is key to global stress response. Furthermore, observed an effect apoCrp on expression absence its effector cAMP. This provides support proposed NAP-like role Crp, suggesting it represents intermediate point evolution a ligand-controlled TF from NAP.
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Abstract In Escherichia coli , one of the best understood microorganisms, much can still be learned about basic interactions between transcription factors and promoters, particularly in stationary phase. When a cAMP-deficient cya mutant is supplied with maltose as main carbon source, mutations develop upstream from two genes malT sdaC . Here, we explore regulation using fluorescence-based genetic reporters combination both spontaneously evolved systematically engineered cis -acting...