A5102958388- Salmonella and Campylobacter epidemiology
- Listeria monocytogenes in Food Safety
- Aquaculture disease management and microbiota
- Vibrio bacteria research studies
- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Escherichia coli research studies
- Identification and Quantification in Food
- Antibiotic Resistance in Bacteria
- Viral gastroenteritis research and epidemiology
- Probiotics and Fermented Foods
- Food Safety and Hygiene
- Bacteriophages and microbial interactions
- Vector-borne infectious diseases
- Mosquito-borne diseases and control
- Innovative Teaching Methods
- COVID-19 Clinical Research Studies
- Microbial Inactivation Methods
- Bacterial Identification and Susceptibility Testing
- Biosensors and Analytical Detection
- Aquaculture Nutrition and Growth
- Oral and gingival health research
- Bacterial Infections and Vaccines
- Pharmaceutical and Antibiotic Environmental Impacts
- Microbial infections and disease research
Hamad Medical Corporation
2023-2024
Hamilton Medical Center
2024
University of Alabama at Birmingham
2022
Society of Critical Care Medicine
2021
Medical Research Associates
2020
Boston University
2020
University of Massachusetts Chan Medical School
2020
St. Mary Medical Center
2020
Brown University
2020
University of Maryland, College Park
2004-2014
ABSTRACT The complete genome of Aeromonas hydrophila ATCC 7966 T was sequenced. , a ubiquitous waterborne bacterium, has been placed by the Environmental Protection Agency on Contaminant Candidate List because its potential to cause human disease. 4.7-Mb this emerging pathogen shows physiologically adroit organism with broad metabolic capabilities and considerable virulence potential. A large array genes, including some identified in clinical isolates spp. or Vibrio spp., may confer upon...
A total of 227 isolates Aeromonas obtained from different geographical locations in the United States and parts world, including 28 reference strains, were analyzed to determine presence various virulence factors. These also fingerprinted using biochemical identification pulse-field gel electrophoresis (PFGE). Of these isolates, 199 that collected water clinical samples belonged three major groups or complexes, namely, A. hydrophila group, caviae-A. media veronii-A. sobria based on profiles,...
The incidence of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) infections is increasing in the United States, and it possible that municipal wastewater could be a reservoir this microorganism. To date, no U.S. studies have evaluated occurrence MRSA wastewater.We examined methicillin-susceptible S. (MSSA) at treatment plants.We collected samples from two Mid-Atlantic Midwest plants between October 2009 2010. Samples were analyzed for MSSA using membrane filtration....
ABSTRACT The potential impact of food animals in the production environment on bacterial population as a result antimicrobial drug use for growth enhancement continues to be cause concern. Enterococci from 82 farms within poultry region eastern seaboard were isolated establish baseline susceptibility profiles number antimicrobials used well clinical environments. Of 541 isolates recovered, Enterococcus faecalis (53%) and E. faecium (31%) predominant species, while multiresistant phenotypes...
ABSTRACT Aeromonas hydrophila has increasingly been implicated as a virulent and antibiotic-resistant etiologic agent in various human diseases. In previously published case report, we described subject with polymicrobial wound infection that included persistent aggressive strain of A . (E1), well more (E2). To better understand the differences between pathogenic environmental strains , conducted comparative genomic functional analyses virulence-associated genes these two isolates (E1 E2),...
In U.S. conventional poultry production, antimicrobials are used for therapeutic, prophylactic, and nontherapeutic purposes. Researchers have shown that this can select antibiotic-resistant commensal pathogenic bacteria on farms in poultry-derived products. However, no studies investigated on-farm changes resistance as transition to organic practices cease using antibiotics.We the prevalence of Enterococcus transitioned practices.Poultry litter, feed, water samples were collected from 10...
ABSTRACT The genomes of 10 Aeromonas isolates identified and designated hydrophila WI, Riv3, NF1 to NF4; A. dhakensis SSU; jandaei Riv2; caviae NM22 NM33 were sequenced annotated. Isolates NF4 from a patient with necrotizing fasciitis (NF). Two environmental (Riv2 -3) the river water which NF acquired infection. While NF2 clonal, was genetically distinct. Outside conserved core these isolates, several unique genomic features identified. most virulent strains possessed one following four...
ABSTRACT Rugose phenotypes, such as those observed in Vibrio cholerae , have increased resistance to chlorine, oxidative stress, and complement-mediated killing. In this study we identified defined a rugose phenotype Salmonella enterica serovar Typhimurium DT104 showed induction only on certain media at 25°C after 3 days of incubation. Incubation 37°C resulted the appearance smooth phenotype. Observation ultrastructure form stable variant (Stv), which was isolated following series passages...
A novel cell wall hydrolase encoded by the murA gene of Listeria monocytogenes is reported here. Mature MurA a 66-kDa surface protein that recognized well-characterized L. monocytogenes-specific monoclonal antibody EM-7G1. displays two characteristic features: (i) an N-terminal domain with homology to muramidases from several gram-positive bacterial species and (ii) four copies wall-anchoring LysM repeat motif present within its C-terminal domain. Purified recombinant produced in Escherichia...
As a group of faculty with expertise and research programs in the area host-pathogen interactions (HPI), we are concentrating on students’ learning HPI concepts. such developed concept inventory to measure level understanding relative after completion set microbiology courses (presently eight courses). Concept inventories have been useful tools for assessing student learning, our interest was develop tool progression courses. Our teaching goal create bridges between which would eliminate...
ABSTRACT The rugose (also known as wrinkled or rdar) phenotype in Salmonella enterica serovar Typhimurium DT104 Rv has been associated with cell aggregation and the ability, at low temperature under low-osmolarity conditions, to form pellicles biofilms. Two Tn 5 insertion mutations genes that are involved lipopolysaccharide (LPS) synthesis, ddhC (A1-8) waaG (A1-9), of resulted diminished expression colony rugosity. Scanning electron micrographs revealed mutant showed reduced amounts...
Isolates of Vibrio parahaemolyticus and V. alginolyticus from human environmental sources were examined for antibiotic susceptibility by the methods minimal inhibitory concentration (MIC) in broth agar diffusion. These strains found to be almost uniformly susceptible chloramphenicol tetracycline within attainable serum levels. The relationships zone sizes MICs these two antimicrobial agents ampicillin conformed essentially those obtained standard with gram-negative rods. Most resistant...
This essay describes how the use of a concept inventory has enhanced professional development and curriculum reform efforts faculty teaching community. The Host Pathogen Interactions (HPI) team is composed research with expertise in HPI who share goal improving learning experience students nine linked undergraduate microbiology courses. To support evidence-based reform, we administered our Concept Inventory as pre- postsurvey to approximately 400 each year since 2006. resulting data include...
As research faculty with expertise in the area of host–pathogen interactions (HPI), we used a group model to effect our professional development as scientific educators. We have established working hypothesis: The implementation curriculum that forms bridges between seven HPI courses allows students achieve deep and meaningful learning concepts. Working collaboratively, identified common goals, chose two microorganisms serve anchors for student learning. instituted variations published...
As reclaimed water use expands, it is important to evaluate potential occupational health risks from exposure this alternative source. We compared odds of colonization with methicillin-resistant Staphylococcus aureus (MRSA), methicillin-susceptible S. (MSSA), vancomycin-resistant enterococci (VRE), and vancomycin-susceptible (VSE) between spray irrigation workers using office worker controls. Nasal dermal swabs 19 24 controls were collected analyzed for MRSA, MSSA, VRE, VSE. Isolates...
ABSTRACT The occurrence of resistance to the streptogramin quinupristin-dalfopristin in Enterococcus faecium isolates from chickens on Eastern Seaboard, was evaluated. Quinupristin-dalfopristin found 51 78% E. food production environment. high level this organism suggests that reservoir may compromise therapeutic potential quinupristin-dalfopristin.