A5004991920- Sphingolipid Metabolism and Signaling
- Asthma and respiratory diseases
- Respiratory and Cough-Related Research
- Cystic Fibrosis Research Advances
- Inhalation and Respiratory Drug Delivery
- Lipid Membrane Structure and Behavior
- Obesity, Physical Activity, Diet
- Dysphagia Assessment and Management
- Air Quality and Health Impacts
- HER2/EGFR in Cancer Research
- Noise Effects and Management
- Smoking Behavior and Cessation
- Ion Channels and Receptors
- Pediatric health and respiratory diseases
- Magnesium in Health and Disease
- Electrolyte and hormonal disorders
- Streptococcal Infections and Treatments
- Aerosol Filtration and Electrostatic Precipitation
- PI3K/AKT/mTOR signaling in cancer
- Cancer, Hypoxia, and Metabolism
- Glycosylation and Glycoproteins Research
- Cell death mechanisms and regulation
- Parathyroid Disorders and Treatments
- Antimicrobial Resistance in Staphylococcus
- Cyclone Separators and Fluid Dynamics
University of Kansas Medical Center
2019-2024
Cornell University
2024
City of Hope
2024
University of Miami
2019-2020
Columbia University
2015
University of California, Davis
2011-2014
Staphylococcus aureus USA300 strains cause a highly inflammatory necrotizing pneumonia. The virulence of this strain has been attributed to its expression multiple toxins that have diverse targets including ADAM10, NLRP3 and CD11b. We demonstrate induction necroptosis through RIP1/RIP3/MLKL signaling is major consequence S. toxin production. Cytotoxicity could be prevented by inhibiting either RIP1 or MLKL mutants lacking agr, hla Hla pore formation, lukAB psms were deficient in inducing...
Rationale: Electronic cigarette (e-cig) use has been widely adopted under the perception of safety. However, possibly adverse effects e-cig vapor in never-smokers are not well understood.Objectives: To test nicotine-containing vapors on airway mucociliary function differentiated human bronchial epithelial cells isolated from and airways a novel, ovine large animal model.Methods: Mucociliary parameters were measured sheep. Systemic nicotine delivery to sheep was quantified using plasma...
Abstract Despite concerns over their safety, e-cigarettes (e-cigs) remain a popular tobacco product. Although nicotine and flavors found in e-cig liquids (e-liquids) can cause harm the airways, whether delivery vehicles propylene glycol (PG) vegetable glycerin (VG) are innocuous when inhaled remains unclear. Here, we investigated effects of aerosols generated from e-liquid containing only PG/VG on airway inflammation mucociliary function primary human bronchial epithelial cells (HBEC) sheep....
Background: Recent successes with immune checkpoint inhibitors (ICIs) and tyrosine kinase (TKIs) for the treatment of solid malignancies have paved way a new era combined therapy. A common side effect seen each these classes is thyroid dysfunction, rates estimated at 30–40% TKI 10–20% ICI. However, little known about ICI+TKI therapy on function. Therefore, this study evaluated incidence, clinical features, risk factors developing abnormalities during relationship to cancer outcomes. Methods:...
Aquaporin 2 (AQP2) is responsible for regulating the concentration of urine in collecting tubules kidney under control vasopressin (Vp).Studies using Vp-deficient Brattleboro rats, however, indicated existence substantial Vp-independent mechanisms membrane insertion, as well transcriptional regulation, this water channel.The mechanism(s) clinically relevant to patients with X-linked nephrogenic diabetes insipidus (NDI) by therapeutically bypassing dysfunctional Vp receptor.On basis studies...
Vegetable glycerin (VG) and propylene glycol (PG) serve as delivery vehicles for nicotine flavorings in most e-cigarette (e-cig) liquids. Here, we investigated whether VG e-cig aerosols, the absence of flavors, impact parameters mucociliary function human volunteers, a large animal model (sheep), air-liquid interface (ALI) cultures primary bronchial epithelial cells (HBECs). We found that VG-containing (VG or PG/VG), but not sole PG-containing, aerosols reduced activity nasal cystic fibrosis...
We previously presented that the neutral sphingomyelinase 2 (nSMase2) is only SMase activated in human airway epithelial (HAE) cells following exposure to oxidative stress (ox-stress), yielding ceramide accumulation and thereby inducing apoptosis. Furthermore, we reported nSMase2 a phospho-protein which level of phosphorylation controls activation induced by ox-stress. Here identify five specific serines are phosphorylated demonstrate their activity upon ox-stress an interdependent manner....
Propylene glycol (PG) is a common delivery vehicle for nicotine and flavorings in e-cigarette (e-cig) liquids largely considered safe ingestion. However, little known about its effects as an e-cig aerosol on the airway. Here, we investigated whether pure PG aerosols realistic daily amounts impact parameters of mucociliary function airway inflammation large animal model (sheep) vivo primary human bronchial epithelial cells (HBECs) vitro. Five-day exposure sheep to 100% increased mucus...
Abstract The EGF receptor (EGFR) is a proto-oncogene commonly dysregulated in several cancers including non–small cell lung carcinoma (NSCLC) and, thus, targeted for treatment using tyrosine kinase inhibitors (TKI) such as erlotinib. However, despite the efficacy observed patients with NSCLC harboring oncogenic variants of EGFR, general ineffectiveness TKIs who are current and former smokers necessitates identification novel mechanisms to overcome this phenomenon. Previously, we showed that...
Aspiration pneumonia is a lower respiratory tract infection that results from inhalation of foreign material, often gastric and oropharyngeal contents. It important to distinguish this similar entity, aspiration with chemical pneumonitis, as treatment approaches may differ. An evolving understanding the human microbiome has shed light on pathogenesis pneumonia, suggesting dysbiosis, repetitive injury, inflammatory responses play role in its development. Risk factors for events involve...
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Alpha-klotho (KL) is an anti-aging protein and has been shown to exert anti-inflammatory anti-oxidative effects in the lung pulmonary diseases such as chronic obstructive disease (COPD) cystic fibrosis. The current study investigated direct effect of KL on bronchial epithelium regards mucociliary clearance parameters. Primary human murine tracheal epithelial cells, cultured differentiated at air liquid interface (ALI), were treated with recombinant or infected a lentiviral vector expressing...
Synopsis: In this unblinded, multicenter, randomized control trial, patients with mild asthma who quadrupled the dose of their inhaled glucocorticoids upon initial signs deterioration experienced fewer severe exacerbations than did not quadruple glucocorticoid dose. Source: McKeever T, Mortimer K, Wilson A, et al. Quadrupling to abort exacerbations. N Engl J Med. 2018;378:902–910.
The use of e-cigarettes has risen in popularity over the past decade, especially among adolescents. E-cigarette is a generic term that covers variety nicotine delivery systems using vapour. These devices were invented and initially marketed as safer alternative to tobacco cigarettes. However, vast advertising campaigns on social media highlighting pleasant flavours made them appealing dearth evidence regarding their safety compelled research efforts understand how e-cigarette use, or...