A5049208889- Neurobiology and Insect Physiology Research
- Cell Image Analysis Techniques
- Cystic Fibrosis Research Advances
- Neonatal Respiratory Health Research
- Tracheal and airway disorders
- Advanced Fluorescence Microscopy Techniques
- Asthma and respiratory diseases
- Autophagy in Disease and Therapy
- Vascular Procedures and Complications
- Smoking Behavior and Cessation
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Respiratory and Cough-Related Research
- Developmental Biology and Gene Regulation
- Cellular transport and secretion
- Respiratory Support and Mechanisms
- Axon Guidance and Neuronal Signaling
- Image Processing Techniques and Applications
- Central Venous Catheters and Hemodialysis
- Acute Myocardial Infarction Research
- Insect and Arachnid Ecology and Behavior
- Medical Image Segmentation Techniques
- Cardiovascular and Diving-Related Complications
- Morphological variations and asymmetry
- Ion Channels and Receptors
- Plant Molecular Biology Research
University of Kansas Medical Center
2019-2025
University of Southern California
2025
Medical College of Wisconsin
2020
University of Miami
2009-2019
University of Kansas
2018
Centre of Biomedical Research
2017
Stanford University
2017
St. Michael's Hospital
2005-2017
Baylor University Medical Center
2011-2013
University Health Network
2013
Dendrite arborization patterns are critical determinants of neuronal function. To explore the basis transcriptional regulation in dendrite pattern formation, we used RNA interference (RNAi) to screen 730 regulators and identified 78 genes involved patterning stereotyped dendritic arbors class I da neurons Drosophila. Most these affect morphology without altering number (da) fall primarily into three groups. Group A control both primary extension lateral branching, hence overall field....
Rationale: Electronic cigarette (e-cig) use has been widely adopted under the perception of safety. However, possibly adverse effects e-cig vapor in never-smokers are not well understood.Objectives: To test nicotine-containing vapors on airway mucociliary function differentiated human bronchial epithelial cells isolated from and airways a novel, ovine large animal model.Methods: Mucociliary parameters were measured sheep. Systemic nicotine delivery to sheep was quantified using plasma...
Abstract Despite concerns over their safety, e-cigarettes (e-cigs) remain a popular tobacco product. Although nicotine and flavors found in e-cig liquids (e-liquids) can cause harm the airways, whether delivery vehicles propylene glycol (PG) vegetable glycerin (VG) are innocuous when inhaled remains unclear. Here, we investigated effects of aerosols generated from e-liquid containing only PG/VG on airway inflammation mucociliary function primary human bronchial epithelial cells (HBEC) sheep....
Dendrites exhibit a wide range of morphological diversity, and their arborization patterns are critical determinants proper neural connectivity. How different neurons acquire distinct dendritic branching during development is not well understood. Here we report that Spineless (Ss), the Drosophila homolog mammalian aryl hydrocarbon (dioxin) receptor (Ahr), regulates dendrite diversity in (da) sensory neurons. In loss-of-function ss mutants, class I II da neurons, which normally characterized...
We describe LOLLIbow, a Brainbow-based live imaging system with applications in developmental biology and neurobiology. The development of an animal, including the environmentally sensitive adaptation its brain, is thought to proceed through continual orchestration among diverse cell types as they divide, migrate, transform interact one another within body. To facilitate direct visualization such dynamic morphogenesis by individual cells vivo, we have modified original Brainbow for...
Same-day discharge after percutaneous coronary intervention (PCI) may be safe for some patients. Few data are available on patient-reported outcomes and preferences same-day PCI.Between March 2008 2010, a total of 298 patients undergoing elective PCI via femoral access at 2 medical centers (Mount Sinai Hospital, New York, NY, Baylor Medical Center, Dallas, TX) were randomized to (n=150) or next-day (n=148) discharge. The primary outcome was high patient coping during the 7 days defined as...
Rationale: CTSS (cathepsin S) is a cysteine protease that observed at higher concentrations in BAL fluid and plasma of subjects with chronic obstructive pulmonary disease (COPD). Objectives: To investigate whether involved the pathogenesis cigarette smoke-induced COPD determine targeting upstream signaling could prevent disease. Methods: expression was investigated animal human tissue cell models COPD. Ctss-/- mice were exposed to long-term smoke forced oscillation expiratory measurements...
Vegetable glycerin (VG) and propylene glycol (PG) serve as delivery vehicles for nicotine flavorings in most e-cigarette (e-cig) liquids. Here, we investigated whether VG e-cig aerosols, the absence of flavors, impact parameters mucociliary function human volunteers, a large animal model (sheep), air-liquid interface (ALI) cultures primary bronchial epithelial cells (HBECs). We found that VG-containing (VG or PG/VG), but not sole PG-containing, aerosols reduced activity nasal cystic fibrosis...
Cigarette smoke (CS) is a leading cause of chronic obstructive pulmonary disease (COPD). Here, we investigated whether the ion channel amplifier nesolicaftor rescues CS-induced mucociliary and dysfunction. Since CS increases expression transforming growth factor-beta1 (TGF-β1), human bronchial epithelial cells (HBEC) from healthy donors were used for TGF-β1 COPD (COPD-HBEC) exposure experiments. induce dysfunction by increasing MUC5AC decreasing conductance important mucus hydration. These...
Abstract Introduction Periorbital burns pose significant risks for complications such as contracture, infection, and ocular damage, potentially threatening vision. In this study, we aim to fill the gaps in literature by examining prevalence, characteristics of presentation, consultation patterns periorbital burns. Methods A retrospective review was conducted all patients admitted a large urban burn center between January 1, 2023 December 31, 2023. Admission records were screened identify...
Improved ventilation strategies have been the mainstay for reducing mortality in acute respiratory distress syndrome. Their unique clinical effectiveness is, however, unmatched by our understanding of underlying mechanobiology, and their impact on alveolar dynamics gas exchange remains largely speculative.To assess changes associated effects local experimental models lung injury (ALI) responsiveness to sighs.Alveolar were studied vivo darkfield microscopy multispectral oximetry murine ALI...
Despite therapeutic progress in treating cystic fibrosis (CF) airway disease, inflammation with associated mucociliary dysfunction remains largely unaddressed. Inflammation reduces the activity of apically expressed large-conductance Ca
Dendrites lengthen by several orders of magnitude during neuronal development, but how membrane is allocated in dendrites to facilitate this growth remains unclear. Here, we report that Ras opposite (Rop), the Drosophila ortholog key exocytosis regulator Munc18-1, an essential factor mediating dendrite growth. Neurons with depleted Rop function exhibit reduced terminal outgrowth followed primary degeneration, suggestive differential requirements for and maintenance different dendritic...
Abstract Background Beginning June 2019, Children's Wisconsin was the first hospital to identify a cohort of adolescent patients hospitalized with symptoms likely associated e‐cigarette use. Our report adds growing literature describing radiographic, gross and cytopathologic bronchoscopic findings, short‐term lung function outcomes in this adolescents or vaping product use injury (EVALI). Methods We present 15 from September, 2019 confirmed EVALI. abstracted data inpatient hospitalization...
Propylene glycol (PG) is a common delivery vehicle for nicotine and flavorings in e-cigarette (e-cig) liquids largely considered safe ingestion. However, little known about its effects as an e-cig aerosol on the airway. Here, we investigated whether pure PG aerosols realistic daily amounts impact parameters of mucociliary function airway inflammation large animal model (sheep) vivo primary human bronchial epithelial cells (HBECs) vitro. Five-day exposure sheep to 100% increased mucus...
Rationale: Coronavirus disease 2019 (COVID-19) can cause disruption of the renin-angiotensin system in lungs, possibly contributing to pulmonary capillary leakage. Thus, angiotensin receptor blockers (ARBs) may improve respiratory failure. Objective: Assess safety losartan for use failure related COVID-19 (NCT04335123). Methods: Single arm, open label trial those hospitalized with COVID-19. Oral (25 mg daily 3 days, then 50 mg) was administered from enrollment until day 14 or hospital...
The spike (S) polypeptide of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) consists the S1 and S2 subunits is processed by cellular proteases at S1/S2 boundary that contains a furin cleavage site (FCS),
Flavorings enhance the palatability of e-cigarettes (e-cigs), with menthol remaining a popular choice among e-cig users. Menthol flavor remains one only flavors approved by United States FDA for use in commercially available, pod-based e-cigs. However, safety inhaled at high concentrations used e-cigs unclear. Here, we tested effects on parameters mucociliary clearance (MCC) air–liquid interface (ALI) cultures primary airway epithelial cells. ALI treated basolateral (1 mM) showed significant...