A5084186413- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Neurotransmitter Receptor Influence on Behavior
- Neuroscience and Neuropharmacology Research
- Pharmacological Receptor Mechanisms and Effects
- Pain Mechanisms and Treatments
- Neuroendocrine regulation and behavior
- Ion channel regulation and function
- Nicotinic Acetylcholine Receptors Study
- Pharmacological Effects and Assays
- Hypothalamic control of reproductive hormones
- Stress Responses and Cortisol
- Traumatic Brain Injury Research
- Chemical Synthesis and Analysis
- Cholinesterase and Neurodegenerative Diseases
- Biochemical effects in animals
- Nerve injury and regeneration
- Adipose Tissue and Metabolism
- Pituitary Gland Disorders and Treatments
- Vitamin K Research Studies
- Vitamin C and Antioxidants Research
- Regulation of Appetite and Obesity
- Parkinson's Disease Mechanisms and Treatments
- Traumatic Brain Injury and Neurovascular Disturbances
- Analytical Chemistry and Chromatography
Uniformed Services University of the Health Sciences
2015-2024
Unifor
2006-2023
Center for Neuroscience and Regenerative Medicine
2013-2017
Astraea Therapeutics (United States)
2016
Torrey Pines Institute For Molecular Studies
2016
Washington University in St. Louis
2016
University of Ferrara
2016
Peking University
2013
Emory University
2013
Jones College
2006
The pituitary peptide beta-lipotropin displays essentially no opoid activity in a preparation of guinea pig ileum or the opiate receptor binding assay. However, fragment, beta-lipotropin-(61-91), with enkephalin sequence (Tyr-Gly-Gly-Phe-Met) at its NH2-terminus, has typical opioid effects these two assays.
Dynorphin-A-(1-8), an opioid peptide widely distributed in the rat central nervous system, is present vasopressin-containing neurosecretory cells terminating neural lobe of pituitary. Electron microscopic immunocytochemistry reveals that dynorphin-A-(1-8) contained within same vesicles as vasopressin and vasopressin-associated neurophysin rat. The results indicate dynorphin may be released pituitary concomitantly with during antidiuretic response.
The distribution of immunoreactive dynorphin (ir-dynorphin) has been determined in dorsal and ventral aspects spinal cord root ganglia rabbit rat. Concentrations are highest root, with intermediate levels low both species. Levels ir-dynorphin relatively uniform over examined segments (vertebrae C2-S3) ganglia. Gel permeation chromatography extracts from revealed at least three components differing molecular size all structures. Multiple unilateral or bilateral rhizotomy C5-T1) rat did not...
The effects of opioid agonists with selectivity for kappa, mu and delta types receptors on the K+-stimulated release [3H]dopamine (DA) from striatum cortex rat guinea pig loaded previously monoamine have been studied. kappa agonist U50488H did not affect base-line [3H]DA measured in 5 mM K+, but produced a dose-dependent inhibition stimulated by 20 K+ slices pig, an IC50 about 0.5 nM each case. In contrast, mu-selective agonist, Tyr-D-Ala-Gly-(Me)Phe-Gly-ol, delta-selective...
Opioids, such as morphine and fentanyl, are widely used for the treatment of severe pain; however, prolonged with these drugs leads to development tolerance can lead opioid use disorder. The "Opioid Epidemic" has generated a drive deeper understanding fundamental signaling mechanisms receptors. It is generally thought that three types receptors (μ, δ, κ) activated by endogenous peptides derived from different precursors: Proopiomelanocortin, proenkephalin, prodynorphin. Posttranslational...
Chronic infusion of morphine to guinea pigs produced selective changes in mu agonist binding properties cerebrocortical membrane preparations. Employing the mu-selective opioid [D-Ala2,MePhe4,Gly-ol5]enkephalin (DAMGO) direct studies and competition labeled antagonist binding, we found that major were a decrease number sites with high affinity for agonist, small reduction total receptor number, loss ability guanosine 5'-[gamma-thio]triphosphate regulate binding. A fraction high-affinity...
The opioid-like neuropeptide nociceptin/orphanin FQ (N/OFQ) and its receptor (NOP) are expressed in the substantia nigra (SN), a brain area containing dopamine neurons that degenerate Parkinson's disease. Endogenous N/OFQ facilitates nigral glutamate release inhibits nigrostriatal transmission motor behavior. Here, we present evidence suggesting endogenous may contribute to Pharmacological blockade of SN N/OFQ-NOP system attenuated parkinsonian-like akinesia/hypokinesia 6-hydroxydopamine...
The effects of prolonged morphine exposure on the mu opioid receptor in 7315c pituitary tumor cell membranes have been examined. Since a low concentration naloxone reversed inhibition forskolin-stimulated adenylyl cyclase induced by mu-selective agonist, Tyr-D-Ala-Gly-MePhe-Gly-ol (DAGO), and high concentrations [D-Pen2-D-Pen5]enkephalin (DPDPE), we suggest that these cells contain homogeneous population receptors coupled to via guanyl nucleotide-binding protein. Studies measuring ability...
To determine the diurnal rhythm of plasma β-endorphin (β-End), 10 healthy male volunteers between ages 20 and 32 yr were studied in a sleep laboratory setting for 24-h period. Blood samples taken through an indwelling catheter at 0800, 1000, 1400, 1800, 2200, 2300, 2400 h then half-hourly until 0730 h, they assayed total β-End-like immunoreactivity (irβ-End), PRL, cortisol. Subjects habituated by spending night before study with electrodes recording place. There was clear variation ir β-End,...
A study has been made of the effects a number drugs which have as common property ability to inhibit RNA or protein synthesis, on development tolerance analgesic morphine, given by intravenous infusion, in rats. Measurements were also incorporation 14 C‐lysine into rat brain protein, and C‐orotic acid RNA. Actinomycin D, 6‐mercaptopurine 5‐fluorouracil reduced acquisition morphine at doses produced significant inhibition orotic Tolerance was only affected after intracerebral administration....
Explosive detonations generate atmospheric pressure changes that produce nonpenetrating blast induced "mild" traumatic brain injury (bTBI). The structural basis for mild bTBI has been extremely controversial. present study applies matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging to track the distribution of gangliosides in mouse tissue were exposed very low level explosive (2.5-5.5 psi peak overpressure). We observed major increases ganglioside GM2 hippocampus,...
Recent studies suggest that opioid peptides may be involved in modulating the hypothalamus-pituitary-gonadal axis at a variety of levels both males and females. We report here presence mRNA coding for peptide precursor prodynorphin rat ovary, uterus, testis. Expression this gene is compared to expression two other genes, proenkephalin proopiomelanocortin, mammalian reproductive tissues. Immunohistochemical analysis reveals testis, prodynorphin- derived are present Leydig cells. The...
Female Wistar rats were allowed to self‐administer nicotine solutions through indwelling jugular vein cannulae for 23 h per day periods from three five weeks. Two response levers available the rats; responding on one lever, designated active produced an immediate infusion of solution or saline. A second lever which had no programmed consequences was introduced as a control locomotor stimulant action low doses nicotine. Baseline rates determined over period week, in Rats then access varying...