A5056395725- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Neurotransmitter Receptor Influence on Behavior
- Phosphodiesterase function and regulation
- Pain Mechanisms and Treatments
- Stress Responses and Cortisol
- Cholinesterase and Neurodegenerative Diseases
- Memory and Neural Mechanisms
- Muscle metabolism and nutrition
- Renin-Angiotensin System Studies
- Estrogen and related hormone effects
- Neuroscience and Neuropharmacology Research
- Adenosine and Purinergic Signaling
- Diet and metabolism studies
- CAR-T cell therapy research
- Congenital Heart Disease Studies
- RNA Interference and Gene Delivery
- Protein Hydrolysis and Bioactive Peptides
- Cognitive Functions and Memory
- Metabolism and Genetic Disorders
- Neuroendocrine regulation and behavior
- Nicotinic Acetylcholine Receptors Study
- Muscle Physiology and Disorders
- Pharmacological Receptor Mechanisms and Effects
- Neurobiology and Insect Physiology Research
University of California, Los Angeles
2020-2025
Icahn School of Medicine at Mount Sinai
2017-2022
Neurobehavioral Systems
2019-2021
Center for Neuro-Oncology
2019
Allen Institute for Brain Science
2018
West Virginia University
2011-2015
Morgantown High School
2015
West Virginia University Hospitals
2015
Bentham Science Publishers (China)
2014
Bentham Science Publishers (United Arab Emirates)
2014
The object recognition test (ORT) is a commonly used behavioral assay for the investigation of various aspects learning and memory in mice. ORT fairly simple can be completed over 3 days: habituation day, training testing day. During training, mouse allowed to explore 2 identical objects. On one objects replaced with novel object. Because mice have an innate preference novelty, if recognizes familiar object, it will spend most its time at Due this preference, there no need positive or...
The object recognition test (ORT) is a commonly used behavioral assay for the investigation of various aspects learning and memory in mice. ORT fairly simple can be completed over 3 days: habituation day, training testing day. During training, mouse allowed to explore 2 identical objects. On one objects replaced with novel object. Because mice have an innate preference novelty, if recognizes familiar object, it will spend most its time at Due this preference, there no need positive or...
Opioids, such as morphine and fentanyl, are widely used for the treatment of severe pain; however, prolonged with these drugs leads to development tolerance can lead opioid use disorder. The "Opioid Epidemic" has generated a drive deeper understanding fundamental signaling mechanisms receptors. It is generally thought that three types receptors (μ, δ, κ) activated by endogenous peptides derived from different precursors: Proopiomelanocortin, proenkephalin, prodynorphin. Posttranslational...
Pain is a multidimensional experience and negative affect, or how much the pain “bothersome”, significantly impacts sufferers' quality of life. It well established that κ opioid system contributes to depressive dysphoric states, but whether this affect precipitated by occurrence chronic remains tenuous. Using model persistent pain, we show quantitative real-time-PCR, florescence in situ hybridization, Western blotting GTPgS autoradiography an upregulation expression function receptors (KORs)...
Carbamoyl phosphate synthetase 1 (CPS1) deficiency, a urea-cycle disorder, results in hyperammonemia initiating sequence of adverse events that can lead to coma and death if not treated rapidly. There is high unmet need for an effective therapeutic this especially early neonatal patients where mortality excessive. However, development adeno-associated virus (AAV)-based approach hampered by large cDNA size protein requirement. We developed oversized AAV vector as gene therapy treat CPS1...
Opioids remain among the most effective pain-relieving therapeutics. However, their long-term use is limited due to development of tolerance and potential for addiction. For many years, researchers have explored underlying mechanisms that lead this decreased effectiveness opioids after repeated use, numerous theories been proposed explain these changes. The widely studied involve alterations in receptor trafficking intracellular signaling. Other possible include recruitment new structural...
Abstract Pathogenic variants in the creatine transporter gene SLC6A8 , reported to represent 2% of all intellectual disabilities males, result a spectrum behavioral abnormalities including developmental delay, disability, and deficit speech. While at present there are no effective treatments available, preclinical development testing therapy other approaches increase brain being actively pursued. In studying mouse model disorder, [ 18 F]fluorodeoxyglucose ([ F]FDG)-based positron emission...
The periaqueductal gray (PAG) mediates the antinociceptive properties of analgesics, including opioids and cannabinoids. Administration either or cannabinoids into PAG induces antinociception. However, most studies characterizing in have been conducted naive animals. Few reported on role CB1 receptors during conditions which would prompt administration namely, pain states.To examine inflammatory pain-induced changes receptor expression function midbrain gray.In this study, we used Complete...
Creatine deficiency disorders are inborn errors of creatine metabolism, an energy homeostasis molecule. One these, guanidinoacetate N-methyltransferase (GAMT) deficiency, has clinical characteristics that include features autism, self-mutilation, intellectual disability, and seizures, with approximately 40% having a disorder movement; failure to thrive can also be component. Along low levels, guanidinoacetic acid (GAA) toxicity been implicated in the pathophysiology disorder. Present-day...
Anxiety disorders are prevalent across the United States and result in a large personal societal burden. Currently, numerous therapeutic pharmaceutical treatment options exist. However, drugs to classical receptor targets have shown limited efficacy often come with unpleasant side effects, highlighting need identify novel involved etiology of anxiety disorders. GPR83, recently deorphanized activated by abundant neuropeptide PEN, has also been identified as glucocorticoid regulated (and named...
In 1990, Rosenburg and colleagues reported their findings from the very first clinical study using gene therapy in U.S. 6 They extracted tumor-infiltrating lymphocytes 5 patients with metastatic melanoma, used a retroviral vector to transduce for resistance neomycin, expanded them vitro, then reinfused back into patients.This demonstration of safety led many other studies involving therapy.However, several failures early 2000s major setbacks field.One biggest came when 18-year-old Jesse...
Cyclic nucleotide phosphodiesterases (PDEs) are a super‐family of enzymes responsible for regulating intracellular levels the second messengers, cyclic AMP (cAMP) and GMP (cGMP). PDE2 is special interest due to its high level expression in forebrain regions, which highly implicated memory processes. In order examine role memory, we trained mice object recognition test (ORT) administered inhibitor Bay60‐7550 30, 60 or 90 min prior training (acquisition), immediately after training, 1 hr 3 hrs...
GPR83 is a recently deorphanized G‐protein coupled receptor (GPCR) found to be widely expressed throughout the brain and immune system of mice. Analysis expression reveals high levels in discrete regions, such as nucleus accumbens, hypothalamus, amygdala hippocampus, regions known involved stress modulation, anxiety, depression, memory. Previous studies have reported that regulated by hormone corticosterone knockout mice are resistant stress‐induced despite having similar increases plasma...
The G‐protein coupled receptor GPR83 is expressed in numerous brain regions, including striatum and hypothalamus, as well the immune system, thymus spleen, of mice. Previously, was classified an orphan GPCR with no known endogenous ligand; we identified neuropeptide PEN, derived from precursor ProSAAS, a selective, high‐affinity ligand for GPR83. Previous studies using ProSAAS or knockout models have shown both to be important modulation stress anxiety, feeding body weight regulation....
Abstract Anxiety disorders are prevalent across the United States and result in a large personal societal burden. Currently, numerous therapeutic pharmaceutical treatment options exist. However, drugs to classical receptor targets have shown limited efficacy often come with unpleasant side effects, highlighting need identify novel involved etiology of anxiety disorders. GPR83, recently deorphanized activated by abundant neuropeptide PEN, has also been identified as glucocorticoid regulated...