Field phenotyping by remote sensing has received increased interest in recent years with the possibility of achieving high-throughput analysis crop fields. Along various technological developments, application machine learning methods for image enhanced potential quantitative assessment a multitude traits. For wheat breeding purposes, assessing production spikes, as grain-bearing organ, is useful proxy measure grain production. Thus, being able to detect and characterize spikes from images...

This study evaluates an aerial and ground imaging platform for assessment of canopy development in a wheat field. The dependence two traits, height vigour, on fertilizer treatment was observed field trial comprised ten varieties spring wheat. A custom-built mobile (MGP) unmanned vehicle (UAV) were deployed at the experimental site standard red, green blue (RGB) image collection five occasions. Meanwhile, reference measurements vigour manually recorded during growing season. Canopy level...

The morphology of plant root anatomical features is a key factor in effective water and nutrient uptake. Existing techniques for phenotyping traits are often based on manual or semi-automatic segmentation annotation microscopic images cross sections. In this article, we propose fully automated tool, hereinafter referred to as RootAnalyzer, efficiently extracting analyzing from root-cross section images. Using range image processing such local thresholding nearest neighbor identification,...

In this paper we report on an automated procedure to capture and characterize the detailed structure of a crop canopy by means stereo imaging. We focus attention specifically characteristic height distribution—canopy shoot area as function height—which can provide elaborate picture growth health under given set conditions. apply method wheat field trial involving ten Australian varieties that were subjected two different fertilizer treatments. A novel camera self-calibration approach is...

In this article, a new method for estimating the volume of selected fruits and vegetables, using information obtained from three orthogonal images object is proposed. The was based on novel lower bound volume, combined with an application well-known upper bound. by calculating artificial created intersection silhouettes. applied to significant range food products simple geometric objects, it found that estimated approximately 11.9% error, generally, 2%, rounded specifically.

In this article we propose a novel tool that takes an initial segmented image and returns more accurate segmentation accurately captures sharp features such as leaf tips, twists axils. Our algorithm utilizes basic a-priori information about the shape of plant leaves local orientations to fit active contour models important have been missed during segmentation. We compare performance our approach with three state-of-the-art techniques, using error metrics. The results show tips are detected...

One of the main challenges associated with image-based field phenotyping is variability illumination. During a single day's imaging session, or between different sessions on days, sun moves in and out cloud cover has varying intensity. How one to know from consecutive images alone if plant become darker over time, weather conditions have simply changed clear overcast? This significant problem address as colour an important phenotypic trait that can be measured automatically images. In this...

As the global population and resource scarcity simultaneously increase, pressure on plant breeders growers to maximise effectiveness of their operations is immense. In this article, we explore usefulness image-based data collection analysis field experiments consisting multiple sites, varieties, treatments. The goal approach determine whether noninvasive acquisition image can be used find relationships between canopy traits environmental factors. Our results are based from three trials in...

In this article, we investigate the relationship between range of optimal parameters active contour models and shape target object. We focus on weights internal external energy terms snakes functional. Our contributions are 3-fold. First, propose a normalization step that brings search space for into bounded range. Secondly, perform systematic study behaviour all possible settings their large set synthetic geometric shapes. introduce concept stability diagrams as novel approach assessing...

Modern genomics experiments are often conducted in controlled environments on hundreds of plants at a time.As such, manual and destructive phenotyping techniques no longer suitable.Recently there has been trend toward automated high-throughput facilities, an attempt to relieve the bottleneck.A majority these facilities focus use cameras for non-destructive recording plant data.Hence, new challenge lies accurately segmenting from 2D images manner then analysing structural statistical...

Abstract Metal nanoparticles sensitize cancers to radiotherapy however their mechanisms of action are complex. The conceptual inspiration arose from theories physical dose deposition various chemical and biological factors have also been identified. Interpretation data has limited by challenges in measuring true DNA damage compared repair factors. Here, we applied a new assay, STRIDE, for the first time measure double strand breaks (DSBs) 4T1 cells as model triple negative breast cancer...