A5011389797- Immune Response and Inflammation
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Intensive Care Unit Cognitive Disorders
- Metabolism and Genetic Disorders
- Immunotherapy and Immune Responses
- Adrenal Hormones and Disorders
- Diabetes Management and Research
- Sepsis Diagnosis and Treatment
- NF-κB Signaling Pathways
- Enhanced Recovery After Surgery
- Body Contouring and Surgery
- Lymphatic System and Diseases
- Parkinson's Disease Mechanisms and Treatments
- Mitochondrial Function and Pathology
- Gastrointestinal motility and disorders
- interferon and immune responses
- Mesenchymal stem cell research
Universitätsmedizin Greifswald
2013-2021
Universität Greifswald
2015
Critically ill patients frequently develop muscle atrophy and weakness in the intensive-care-unit setting [intensive care unit-acquired (ICUAW)]. Sepsis, systemic inflammation, acute-phase response are major risk factors. We reported earlier that protein serum amyloid A1 (SAA1) is increased accumulates of ICUAW patients, but its relevance was unknown. Our objectives were to identify SAA1 receptors their downstream signalling pathways myocytes skeletal investigate role inflammation-induced...
The role of regulatory T cells (Tregs) in bacterial sepsis remains controversial because antibody-mediated depletion experiments gave conflicting results. We employed DEREG mice (DEpletion REGulatory cells) and a caecal ligation puncture model to elucidate the CD4+Foxp3+ Tregs sepsis. In natural can be visualized easily selectively depleted by diphtheria toxin animals express receptor enhanced green fluorescent protein as fusion under control foxp3 locus. confirmed rapid Treg-activation an...
The local anesthetic lidocaine, which has been used extensively during liposuction, reported to have cytotoxic effects and therefore would be unsuitable for use in autologous lipotransfer. We evaluated the effect of lidocaine on distribution, number, viability adipose-derived stem cells (ASCs), preadipocytes, mature adipocytes, leukocytes fatty fluid portion lipoaspirate using antibody staining flow cytometry analyses. Adipose tissue was harvested from 11 female patients who underwent...
During sepsis, CD4 T cells express activation markers within the first 24 h. In present study, mechanisms of T-cell and its consequences were addressed in an acute peritonitis model mice. The response CD4+ to sepsis induction was compared between OTII mice, characterized by ovalbumin-specific receptor-transgenic cells, C57BL/6 controls (wild type [WT] mice). Because ovalbumin absent during peritonitis, could not be activated canonical antigen recognition. both WT control effector Foxp3+...
Sepsis is frequently complicated by a state of profound immunosuppression, in its extreme form known as immunoparalysis. We have studied the role adaptive immune system murine acute peritonitis model. To read out we primed post-septic and control animals immunization with model antigen TNP-ovalbumin alum, measured specific antibody-responses via ELISA ELISpot assay well T-cell responses proliferation after restimulation. Specific antibody titers, affinity plasma cell counts bone marrow were...
A20 is an ubiquitin-editing enzyme that ensures the transient nature of inflammatory signaling pathways induced by cytokines like TNF-α and IL-1 or pathogens via Toll-like receptor (TLR) pathways. It has been identified as a negative regulator dendritic cell (DC) maturation attenuator their immunostimulatory properties. Ex vivo A20-depleted cells showed enhanced expression pro-inflammatory costimulatory molecules, which resulted in hyperactivation tumor-infiltrating T lymphocytes inhibition...
Abstract Postoperative peritonitis is characterized by a more severe clinical course than other forms of secondary peritonitis. The pathophysiological mechanisms behind this phenomenon are incompletely understood. This study used an innovative model to investigate these mechanisms, combining the models murine Colon Ascendens Stent Peritonitis (CASP) and Surgically induced Immune Dysfunction (SID). Moreover, influence previously described anti-inflammatory reflex transmitted vagal nerve was...
Although antigen-specific priming of antibody responses is impaired during sepsis, there nevertheless a strong increase in IgM and IgG serum concentrations. Using colon ascendens stent peritonitis (CASP), mouse model polymicrobial abdominal we observed substantial increases as well all subclasses, starting at day three peaking two weeks after sepsis induction. The dominant source antibody-secreting cells was by far the spleen, with minor contribution mesenteric lymph nodes. Remarkably,...