A5028794353- Sphingolipid Metabolism and Signaling
- Immune cells in cancer
- Immune Cell Function and Interaction
- Inflammasome and immune disorders
- Microscopic Colitis
- Pancreatitis Pathology and Treatment
- Inflammatory Bowel Disease
- Cardiac, Anesthesia and Surgical Outcomes
- Immune Response and Inflammation
- Enhanced Recovery After Surgery
- Trace Elements in Health
- Pneumothorax, Barotrauma, Emphysema
- Platelet Disorders and Treatments
- Endoplasmic Reticulum Stress and Disease
- Dermatological and Skeletal Disorders
- Parathyroid Disorders and Treatments
- Nosocomial Infections in ICU
- Autoimmune and Inflammatory Disorders
- Gastrointestinal motility and disorders
- IL-33, ST2, and ILC Pathways
- Pressure Ulcer Prevention and Management
- Pancreatic and Hepatic Oncology Research
- Intensive Care Unit Cognitive Disorders
- Prion Diseases and Protein Misfolding
- Pleural and Pulmonary Diseases
Universitätsmedizin Greifswald
2013-2024
Klinikum rechts der Isar
2017-2023
Technical University of Munich
2023
Max Delbrück Center
2010-2018
Universitätsmedizin Göttingen
2014
Universität Greifswald
2013
DRK-Blutspendedienst Baden-Württemberg - Hessen
2011
Heidelberg University
2011
Charité - Universitätsmedizin Berlin
2005-2006
Humboldt-Universität zu Berlin
2005
Although predominantly expressed on lymphocytic and hematopoietic cells, the role of sphin-gosine-1-phospate receptor 4 (S1P4) in immune homeostasis is still poorly understood. In this report, we used a S1P4-deficient murine model to characterize biological S1P4-mediated S1P signaling system. S1p4-/- animals showed normal peripheral lymphocyte numbers regular architecture secondary lymphoid organs. Interestingly, S1P4 only marginally affects T-cell function vivo. contrast, dendritic cell...
Megakaryocytes, which mature from hematopoietic progenitors in the bone marrow, further differentiate by reorganizing their cytoplasm into long proplatelet extensions that release platelets circulation. The molecular mechanisms underlying this highly dynamic cytoplasmic and cytoskeletal remodeling process are only poorly understood. Here we report sphingosine 1-phosphate receptor 4 (S1P(4)) is specifically up-regulated during development of human megakaryocytes progenitor cells expressed...
Introduction . Macrophages are key players in complex biological processes. In response to environmental signals, macrophages undergo polarization towards a proinflammatory (M1) or anti-inflammatory (M2) phenotype. Sphingosine 1-phosphate (S1P) is bioactive lysophospholipid that acts via 5 G-protein coupled receptors (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mrow><mml:msub><mml:mrow><mml:mi mathvariant="normal">S</mml:mi><mml:mn...
The expression of the cellular form prion protein (PrP c ) gene is required for replication and neuroinvasion in transmissible spongiform encephalopathies. identification cell types expressing PrP necessary to understanding how agent replicates spreads from peripheral sites central nervous system. To determine nature , a green fluorescent reporter was expressed transgenic mice under control 6.9 kb bovine regulatory sequences. It shown that as two populations mRNA differing by alternative...
Megakaryocytes, which mature from hematopoietic progenitors in the bone marrow, further differentiate by reorganizing their cytoplasm into long proplatelet extensions that release platelets circulation. The molecular mechanisms underlying this highly dynamic cytoplasmic and cytoskeletal remodeling process are only poorly understood. Here we report sphin-gosine 1-phosphate receptor 4 (S1P4) is specifically up-regulated during development of human megakaryo-cytes progenitor cells expressed...
Introduction: Sphingosine-1-phosphate (S1P) regulates the migration of follicular B cells (B2 cells) and directs positioning Marginal zone (MZ within spleen. The function S1P signalling in third cell lineage, B1 cells, mainly present pleural peritoneal cavity, has not yet been determined. Methods: receptor expression was analysed by real-time polymerase chain reaction (qPCR). chemotactic response to studied vitro. role further explored a s1p₄-/- mouse strain. Results: Peritoneal expressed...
Abstract Expression of the cellular prion protein (PrP c ) by host cells is required for replication and neuroinvasion in transmissible spongiform encephalopathies. As a consequence, identification cell types expressing PrP necessary to determine target involved cerebral propagation diseases. To identify mouse brain, immunocytochemical localization was investigated at ultrastructural levels several brain regions. In addition, we analyzed expression pattern green fluorescent reporter gene...
Pancreatic cancer is known for its tumor microenvironment (TME), which rich in stromal and immune cells supporting growth therapy resistance. In particular, tumor-associated macrophages (TAMs) are their angiogenesis- metastasis-promoting properties, lead to the failure of conventional therapies pancreatic cancer. Hence, treatment options targeting TAMs needed. The C-C chemokine receptor type 4 (CCR4) critical cell recruitment into TME, this paper we explore effects genetic or...
Gram-negative infections of the peritoneal cavity result in profound modifications B cell populations and induce migration cells to distant secondary lymphoid organs. However, mechanisms controlling egress from their subsequent trafficking remain incompletely understood. Sphingosine-1-phosphate (S1P)-mediated signaling controls migratory processes numerous immune cells. The present work investigates role S1P-mediated under inflammatory conditions. Differential S1P receptor expression after...
Diversion colitis is a significant health problem due to its high incidence in patients with diverting enterostomy. This mucosal inflammation presents characteristic histopathological features allowing for the differentiation of this entity from other inflammatory bowel diseases. The pathophysiology disease remains ill-defined, part lack appropriate animal models. present study was performed order develop and characterize murine model diversion colitis.A loop colostomy C57BL/6 mice either...
Abstract Postoperative peritonitis is characterized by a more severe clinical course than other forms of secondary peritonitis. The pathophysiological mechanisms behind this phenomenon are incompletely understood. This study used an innovative model to investigate these mechanisms, combining the models murine Colon Ascendens Stent Peritonitis (CASP) and Surgically induced Immune Dysfunction (SID). Moreover, influence previously described anti-inflammatory reflex transmitted vagal nerve was...
Aseptic bypass graft and graft-preserving open local treatment have proved to be the 2 suitable procedures for therapy of infected grafts in vascular surgery (stage III Szilagyi) with preservation limb extremity. After 685 reconstructive operations (1982 1984) chronic arterial occlusive disease supraaortic as well iliac femoral segments, 9 infections involving (1.31%) occurred. These were more common superficial extra-anatomical bypass. The incidence inguinal infection was 0.44%. In cases an...
FTY720 is an immunosuppressive molecule licensed for the treatment of chronic relapsing multiple sclerosis (MS). It attenuates adaptive immune response by sequestering T cells within secondary lymphoid organs via its action as functional antagonist sphingosine-1-phasphate. To date, it unknown whether FTY-induced lymphopenia puts MS patients at increased risk severe forms postoperative infectious complications such abdominal sepsis.To determine effect FTY720-induced on survival to sepsis...
Respiratory distress is a feared complication after thyroid surgery. Differential diagnosis includes bilateral recurrent laryngeal nerve palsy (BRLNP), local hematoma, vocal cord edema and trauma. BRLNP results from intraoperative irritation without physical injury (neurapraxia), or partial complete transsection (axonotmesis neurotmesis, respectively) of the (RLN). RLN typically manifests immediately in postoperativecourse.However, rare cases there delayed, progressive development BRLNP,...
Introduction Splenic B cells exhibit a high expression of the G protein-coupled sphingosine-1-phosphate (S1P) receptor type 4 (S1PR ). Little is known about functional relevance S1PR on those cells. Methods In this study, -deficient mice were used to study role -mediated S1P signaling in cell motility vitro and for maintenance splenic architecture under steady state conditions as well polymicrobial abdominal sepsis vivo . Finally, impact deficiency antibody production after immunization with...
Stress ulcer prophylaxis (SUP) has been a widespread practice both in intensive care units (ICU) and internal wards at the beginning of twenty-first century. Clinical data suggests an important overuse acid suppressive therapy (AST) for this indication. Data on current clinical SUP surgical patients non-ICU setting are spares. In light growing number reports serious side effects AST, study evaluates use AST normal ward German university hospital.Between January 2016 June 2016, was analysed...
B-1 cells are crucially involved in immune defense and regulation of inflammation autoimmunity. predominantly located the peritoneal pleural cavities, although body cavity recirculate systemically under steady-state conditions. The chemokines CXCL12 CXCL13 have been identified as main regulators B-cell trafficking. In mice deficient for sphingosine-1-phosphate receptor 4 (S1PR