A5102957627- Chemokine receptors and signaling
- T-cell and B-cell Immunology
- Cell Adhesion Molecules Research
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Asthma and respiratory diseases
- Mast cells and histamine
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Cytokine Signaling Pathways and Interactions
- Receptor Mechanisms and Signaling
- Glycosylation and Glycoproteins Research
- interferon and immune responses
- NF-κB Signaling Pathways
- Dermatology and Skin Diseases
- Allergic Rhinitis and Sensitization
- Respiratory and Cough-Related Research
- Platelet Disorders and Treatments
- Melanoma and MAPK Pathways
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Systemic Lupus Erythematosus Research
- Peptidase Inhibition and Analysis
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Sphingolipid Metabolism and Signaling
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
Sequoia Sciences (United States)
2024
Epigen Biosciences (United States)
2023
Tracon Pharmaceuticals (United States)
2012-2019
Bayer (United States)
2014
Kyoto Medical Center
2010
University of Portland
2009
Bayer (Germany)
2001-2007
Boehringer Ingelheim (Spain)
2007
Bayer (Japan)
2002-2005
Torrey Pines Institute For Molecular Studies
2005
A recently identified chemokine, fractalkine, is a member of the chemokine gene family, which consists principally secreted, proinflammatory molecules. Fractalkine distinguished structurally by presence CX3C motif as well transmembrane spanning and mucin-like domains shows atypical constitutive expression in number nonhematopoietic tissues, including brain. We undertook an extensive characterization this its receptor CX3CR1 brain to gain insights into use chemokine-dependent systems central...
In this study, the cytokine-producing profile of progenitor T cells (pro-T cells) was determined. During screening a complementary DNA library generated from activated mouse pro-T cells, cytokine designated lymphotactin discovered. Lymphotactin is similar to members both Cys-Cys and Cys-X-Cys chemokine families but lacks two four cysteine residues that are characteristic chemokines. also expressed in CD8+ CD4-CD8- cell receptor alpha beta + thymocytes. It has chemotactic activity for...
Lymphocyte trafficking is an essential process in immune and inflammatory functions which can be thought to contain at least two main components: adhesion migration. Whereas molecules such as the selections are known mediate homing of leukocytes from blood endothelium, chemoattractant substances responsible for migration specific subsets lymphocytes sites infection or inflammation largely unknown. Here we show that chemokine (for cytokine) superfamily, human macrophage protein 1 alpha (MIP-1...
The chemokine RANTES induced biphasic mobilization of Ca 2+ in T cells. initial peak, a transient increase cytosolic mediated by heterotrimeric guanine nucleotide-binding protein (G protein)-coupled pathway, was associated predominantly with chemotaxis. second release and sustained influx dependent on tyrosine kinases, spectrum cellular responses—Ca channel opening, interleukin-2 receptor expression, cytokine release, cell proliferation—characteristic activation. Other chemokines did not...
Molecular analyses of the chemokine fractalkine and its receptor CX3C-R1 in rat brain have revealed a striking polarization: is expressed constitutively neurons up-regulated by TNF-alpha IL-1beta astrocytes. Expression specific receptor, CX3C-R1, restricted to astrocytes microglia. We analyzed functional correlates this expression demonstrate that induces microglial cell migration activation. However, activity on may also be highly relevant inducing astrocyte-microglia interactions through...
Several chemoattractant receptors can support agonist-induced, integrin-dependent arrest of rolling neutrophils in inflamed venules vivo, as well subsequent crawling into tissues. It has been hypothesized that the Galpha(i)-linked subfamilies, especially for chemokines, may mediate parallel activation-dependent homing lymphocyte subsets. However, although several chemokines attract subsets B or T cells, robust triggering resting adhesion to vascular ligands not observed. To study biology...
Chemokines play a central role in immune and inflammatory responses. It has been observed recently that certain viruses have evolved molecular piracy mimicry mechanisms by encoding synthesizing proteins interfere with the normal host defense response. One such viral protein, vMIP-II, encoded human herpesvirus 8, identified vitro antagonistic activities against CC CXC chemokine receptors. We report here vMIP-II additional activity CX3CR1, receptor for fractalkine. To investigate potential...
We describe the isolation of a cDNA that encodes human lymphotactin (Ltn), new class lymphocyte-specific chemokine. Human Ltn shows similarity to some members C-C chemokine family but has lost first and third cysteine residues are characteristic C-X-C chemokines. is chemotactic for lymphocytes not monocytes, makes it unique among In addition, calcium flux desensitization studies indicate uses receptor. The gene maps different chromosome than do families. Taken together, these characteristics...
Spleen tyrosine kinase (Syk) plays essential roles in receptors for Fc portion of immunoglobulins and B cell receptor complex signaling various inflammatory cells; therefore, inhibitors Syk may show potential as antiasthmatic/allergic therapeutics. We identified 2-[7-(3,4-dimethoxyphenyl)-imidazo[1,2-c]pyrimidin-5-ylamino]-nicotinamide dihydrochloride (BAY 61-3606), a potent (Ki = 7.5 nM) selective inhibitor kinase. BAY 61-3606 inhibited not only degranulation (IC50 values between 5 46 but...
Naive T cells are selectively recruited from the blood into peripheral lymph nodes during lymphocyte recirculation. L-selectin, a lectin-like receptor, mediates initial attachment of lymphocytes to high endothelial venules (HEV) in nodes. A subsequent step involving activation beta 2 integrins has been proposed facilitate firm adhesion, but activating signals poorly understood. We report here that either antibody-mediated cross-linking L-selectin on human or treatment with GlyCAM-1, an...
Fractalkine is a CX3C-family chemokine, highly and constitutively expressed on the neuronal cell surface, for which clear CNS physiological function has yet to be determined. Its cognate receptor, CX3CR-1, microglia, brain-resident macrophages; however, these cells do not express fractalkine. We now show that treatment of microglia with fractalkine maintains survival inhibits Fas ligand-induced death in vitro. Biochemical characterization indicates this occurs via mechanisms may include 1)...
Fractalkine is an endothelial cell-derived CX3C chemokine that chemotactic mainly to mononuclear cells. was induced in rat aortic cells (RAEC) by interleukin-1beta (IL-1beta), tumor necrosis factor alpha (TNF-alpha), and lipopolysaccharide (LPS) transcriptionally translationally. This induction correlated with increased NF-kappaB DNA binding activity as determined gel mobility shift assay. Supershift assays revealed the subunits p50 p65 were responsible for kappaB binding. Accordingly, we...