Abstract Substantial evidence does not support the prevailing view that leptin, acting through a hypothalamic relay, decreases bone accrual by inhibiting formation. To clarify mechanisms underlying regulation of architecture we evaluated growth and turnover in wild-type (WT) mice, leptin receptor-deficient db/db leptin-deficient ob/ob mice treated with leptin. We also performed gene therapy to determine effect elevated levels on osteoblasts. Finally, effects loss peripheral signaling...

This study deals with the role of neuropeptide Y (NPY) in regulation cell proliferation. NPY is expressed normal and tumoral prostate, but no data on its possible prostate cancer (PCa) progression are available. Therefore, we evaluated direct effect growth human PCa lines LNCaP (androgen dependent) DU145 PC3 independent). All Y1-R gene protein. treatment reduced proliferation cells increased that cells. The antagonist BIBP3226 abolished such effects, suggesting a mandatory this process....

The amygdala is rich in melanocortin 4 receptors. Because the reduction dietary fat intake after enterostatin injected central nucleus of (CeA) blocked by a receptor antagonist, we investigated role activity CeA regulating food and macronutrient choice. Sprague-Dawley rats, fitted with cannulas, were fed either chow, high-fat (HF) diet, or adapted to two-choice HF low-fat (LF) diet. Injections MC4R agonist melanotan II (MTII) had dose-dependent inhibitory effect on that lasted for at least...

We propose the LOU/c/jall rat as a possible model for research into aging. Physiological and behavioral data have been collected over past 5 years, using lifelong cross-sectional studies. The median life span of rats was 29 months in males 33–34 females. A low level body fat throughout observed both sexes. Basic phenomena aging such weight loss, decrease caloric intake, dramatic drop protein selection were noted from age 18 28 decline muscle mass, depending on sex type muscle, seen. These...

Intracerebroventricular insulin decreases food intake (FI). The central bed nucleus of the amygdala (CeA), as other regions brain regulating feeding behavior, expresses receptors. Our objectives were to show an anorectic response in amygdala, study effect high-fat diets on this response, and map neural network activated by CeA using c-Fos immunohistochemistry. Sprague-Dawley (SD) rats fitted with unilateral cannulas adapted a low-fat (LFD) diet before they fed (HFD). Their saline or (8 mU)...

Little is known about how the ageing process affects pain sensitivity and a relevant animal model therefore required. The effect of age on reactivity in animals has been investigated by several experimenters but results are conflicting. Four groups male female Lou/C/Jall rats (4–29 months old) were used for our study. tests based evaluation reflex or more integrated behaviours after thermal (tail immersion test) mechanical (paw pressure test von Frey stimulation used. With stimulus,...

Abstract Hypothalamic leptin gene therapy normalizes the mosaic skeletal phenotype of leptin-deficient ob/ob mice. However, it is not clear whether increased hypothalamic alters bone metabolism in animals already producing hormone. The objective this study was to evaluate long duration effects recombinant adeno-associated virus-rat (rAAV-Lep) on weight gain and growing skeletally mature leptin-replete female Sprague-Dawley rats. Rats were either unoperated or implanted with cannulas third...

Abstract Objective: In this study, we tested the hypothesis that insufficiency of leptin restraint in hypothalamus is responsible for promoting weight gain and adiposity after ovariectomy (ovx). Whether increasing transgene expression can overcome diminution was evaluated ovx rats. Research Methods Procedures: Enhanced or green fluorescent protein (GFP; control) induced by a single intracerebroventricular injection recombinant adeno‐associated viral vector encoding either gene ( rAAV‐lep )...

Abstract Objective: To investigate the signaling mechanisms that might underlie loss of anorectic response to insulin injections into central nucleus amygdala (CeA) within 3 days feeding a high fat diet. Design and Methods: Protein samples from hypothalamus rats fed or low diets were subjected phosphorylation screening assay. The effects dietary intake on expression activation protein kinase C theta (PKCθ) in brain regions was studied. Finally, lentiviral vectors used overexpress rat PKCθ...

Abstract Objective: Increased leptin transgene expression locally in hypothalamic sites suppresses weight and energy intake, enhances thermogenic expenditure, differentially modulates metabolic hormones for an extended period. We evaluated whether a similar localized of the dorsal vagal complex (DVC) caudal brain stem that also displays biologically relevant receptor would reproduce these varied responses thus demonstrate functional connectivity between hypothalamus DVC. Research Methods...