A5089737556- Pluripotent Stem Cells Research
- Cancer Cells and Metastasis
- Prostate Cancer Treatment and Research
- Mesenchymal stem cell research
- Cancer, Hypoxia, and Metabolism
- 3D Printing in Biomedical Research
- bioluminescence and chemiluminescence research
- Head and Neck Cancer Studies
- Bladder and Urothelial Cancer Treatments
- Advanced Proteomics Techniques and Applications
- Prostate Cancer Diagnosis and Treatment
- Colorectal and Anal Carcinomas
- Tissue Engineering and Regenerative Medicine
- Epigenetics and DNA Methylation
- Retinal and Optic Conditions
- Nutrition and Health in Aging
- Redox biology and oxidative stress
- Chromatin Remodeling and Cancer
- Lung Cancer Research Studies
- Lung Cancer Treatments and Mutations
- Advanced biosensing and bioanalysis techniques
- Digital Imaging for Blood Diseases
- Artificial Intelligence in Healthcare and Education
- Glutathione Transferases and Polymorphisms
- Ferroptosis and cancer prognosis
Johns Hopkins Hospital
2022
University of Washington
2002-2021
Johns Hopkins University
2021
Johns Hopkins Medicine
2021
BC Cancer Agency
2020-2021
University of British Columbia
2019-2020
Seattle University
2010-2018
Fred Hutch Cancer Center
2011
Cleveland Clinic
2011
University of California, Los Angeles
1977-1978
Low oxygen levels have been shown to promote self-renewal in many stem cells. In tumors, hypoxia is associated with aggressive disease course and poor clinical outcomes. Furthermore, tumors display gene expression signatures characteristic of human embryonic cells (hESC). We now tested whether might be responsible for the hESC signature observed tumors. show that hypoxia, through hypoxia-inducible factor (HIF), can induce an hESC-like transcriptional program, including induced pluripotent...
Phenotype-based screening can identify small molecules that elicit a desired cellular response, but additional approaches are required to characterize their targets and mechanisms of action. Here, we show compound termed LCS3, which selectively impairs the growth human lung adenocarcinoma (LUAD) cells, induces oxidative stress. To target mediates this effect, use thermal proteome profiling (TPP) uncover disulfide reductases GSR TXNRD1 as targets. We confirm through enzymatic assays LCS3...
Lineage transformation between lung cancer subtypes is a poorly understood phenomenon associated with resistance to treatment and poor patient outcomes. Here, we aimed model this transition define underlying biological mechanisms identify potential avenues for therapeutic intervention. Small cell (SCLC) neuroendocrine in identity and, contrast non-SCLC (NSCLC), rarely contains mutations that drive the MAPK pathway. Likewise, NSCLCs transform SCLC concomitantly development of therapy...
Abstract Background Patients receiving chemoradiotherapy for head and neck cancer (HNC) are often malnourished. We assessed the utility of nutritional risk index (NRI) in HNC patients undergoing chemoradiotherapy. Methods A population‐based retrospective review treated with curative chemoradiation was performed. Demographics, anthropometrics, overall survival (OS), composite treatment complication rate (G‐tube dependence, radiation incompletion, 90‐day mortality, unplanned hospitalization)...
A 169-nucleotide region from the rabbit beta globin gene has been sequenced by analysis of complementary DNA's cloned in bacterial plasmids. Comparison these sequences with those established for this other techniques provides evidence a high degree fidelity and allows unambiguous establishment that plasmids do not contain harmful sequences.
Identification of effector targets is imperative to the characterization mechanisms action novel small molecules. Here, we describe steps identify drug-protein interactions in lysates derived from cancer cell lines using a thermal proteome profiling (TPP) protocol. Building on existing TTP approaches, detail use an in-solution trypsin digestion technique streamline sample preparation, nonparametric analysis rank proteins for prioritization, and follow-up strategy identifying interactors. For...
Lignostilbene-α,β-dioxygenase A (LsdA) from the bacterium Sphingomonas paucimobilis TMY1009 is a nonheme iron oxygenase that catalyzes cleavage of lignostilbene, compound arising in lignin transformation, to two vanillin molecules. To examine LsdA's substrate specificity, we heterologously produced dimeric enzyme with help chaperones. When tested on several substituted stilbenes, LsdA exhibited greatest specificity for lignostilbene (kcatapp = 1.00 ± 0.04 × 106 m−1 s−1). These experiments...
The first ever insurance reimbursement for an artificial intelligence (AI) system, which expedites triage of acute stroke, occurred in 2020 when the Centers Medicare and Medicaid Services (CMS) granted approval a New Technology Add-on Payment (NTAP). Key aspects AI system that led to its by CMS included unique mechanism action, use robotic process automation, clear linkage system's output clinical outcomes. specific strategies employed encompass first-case scenario proving reimbursable value...
We aimed to examine the change in adherence annual diabetic eye disease (DED) testing an integrated healthcare system (Johns Hopkins Community Physicians 30+ community based primary care clinics) before and after deployment of autonomous artificial intelligence (AI). Adherence DED is defined as completed evaluation by either a human ophthalmology provider, or AI within given calendar year. During COVID pandemic, (IDx‐DR, Digital Diagnostics, Coralville, IA) was deployed at multiple clinics,...
Abstract High-throughput phenotype-based screening of large libraries novel compounds without known targets can identify small molecules that elicit a desired cellular response, but additional approaches are required to find and characterize their mechanisms action. Here we show compound termed lung cancer screen 3 (LCS3), previously selected for its ability impair the growth human adenocarcinoma (LUAD) cell lines, not normal cells, induces oxidative stress activates NRF2 signaling pathway...
Abstract Stable-isotope dilution coupled to targeted mass spectrometry (MS), applying selected reaction monitoring (SRM), provides good selectivity, reproducibility, sensitivity, and multiplexing capability rendering it highly suitable for robust, high-throughput cost-effective verification of cancer biomarker candidates. The ability SRM-MS detect quantify low abundance proteins present in blood plasma/serum at ng/mL or even lower levels, however, relies on the front-end enrichment through...
You have accessJournal of UrologyProstate Cancer: Basic Research & Pathophysiology I1 Apr 2016MP62-18 CONVERSION OF ADENOCARCINOMA TO SMALL CELL CARCINOMA-LIKE BY REPROGRAMMING Gisely Borges, Eneida Vencio, Sue-Ing Quek, Adeline Chen, Diego Salvanha, Ricardo Holly Nguyen, Robert Vessella, Christopher Cavanaugh, Carol Ware, Pamela Troisch, and Alvin Liu BorgesGisely Borges More articles by this author , VencioEneida Vencio QuekSue-Ing Quek ChenAdeline Chen SalvanhaDiego Salvanha VencioRicardo...
You have accessJournal of UrologyScience & Technology Posters1 Apr 2016S&T-10 PROSTATE CANCER DIAGNOSIS BY MULTIPLE SECRETED PROTEIN BIOMARKERS IN VOIDED URINE Tujin Shi, Yuqian Gao, Wei-jun Qian, Robin Leach, Ian Thompson, Sue-Ing Quek, William Ellis, Elizabeth Vitello, and Alvin Liu ShiTujin Shi More articles by this author , GaoYuqian Gao QianWei-jun Qian LeachRobin Leach ThompsonIan Thompson QuekSue-Ing Quek EllisWilliam Ellis VitelloElizabeth Vitello LiuAlvin View All Author...
Supplementary Methods, Figures 1-11, Table Legends 1-7 from HIF Induces Human Embryonic Stem Cell Markers in Cancer Cells
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