A5075753686- Glioma Diagnosis and Treatment
- Meningioma and schwannoma management
- Neurofibromatosis and Schwannoma Cases
- Neuroblastoma Research and Treatments
- Pluripotent Stem Cells Research
- Brain Metastases and Treatment
- 3D Printing in Biomedical Research
- Cancer Cells and Metastasis
- Radiomics and Machine Learning in Medical Imaging
- Epigenetics and DNA Methylation
- Bone Tumor Diagnosis and Treatments
- Cancer, Hypoxia, and Metabolism
- Oral and Maxillofacial Pathology
- Innovative Microfluidic and Catalytic Techniques Innovation
- Sarcoma Diagnosis and Treatment
- Mesenchymal stem cell research
- Cancer Genomics and Diagnostics
- Neurogenesis and neuroplasticity mechanisms
- Head and Neck Surgical Oncology
- Medical Imaging Techniques and Applications
- Lung Cancer Research Studies
- Advanced MRI Techniques and Applications
- Hedgehog Signaling Pathway Studies
- Ferroptosis and cancer prognosis
- MicroRNA in disease regulation
University of Washington
2013-2024
Methodist Hospital
2010-2024
Neurological Surgery
2011-2024
Houston Methodist
2017-2024
Cornell University
2020-2024
Weill Cornell Medicine
2020-2024
Metropolitan University
2023
University of Washington Medical Center
2004-2019
Harborview Medical Center
1997-2017
Swedish Medical Center
2015
Malignant tumors shed DNA into the circulation. The transient half-life of circulating tumor (ctDNA) may afford opportunity to diagnose, monitor recurrence, and evaluate response therapy solely through a non-invasive blood draw. However, detecting ctDNA against normally occurring background cell-free derived from healthy cells has proven challenging, particularly in non-metastatic solid tumors. In this study, distinct differences fragment length size between ctDNAs normal are defined. Human...
Glioblastoma is an aggressive brain tumor that carries a poor prognosis. The tumor's molecular and cellular landscapes are complex, their relationships to histologic features routinely used for diagnosis unclear. We present the Ivy Atlas, anatomically based transcriptional atlas of human glioblastoma aligns individual with genomic alterations gene expression patterns, thus assigning information most important morphologic hallmarks tumor. its clinical database freely accessible online data...
Low oxygen levels have been shown to promote self-renewal in many stem cells. In tumors, hypoxia is associated with aggressive disease course and poor clinical outcomes. Furthermore, tumors display gene expression signatures characteristic of human embryonic cells (hESC). We now tested whether might be responsible for the hESC signature observed tumors. show that hypoxia, through hypoxia-inducible factor (HIF), can induce an hESC-like transcriptional program, including induced pluripotent...
Abstract Background Tumor cell invasion into adjacent normal brain is a mesenchymal feature of GBM and major factor contributing to their dismal outcomes. Therefore, better understandings mechanisms that promote change in are great clinical importance address invasion. We previously showed the bHLH transcription TWIST1 which orchestrates carcinoma metastasis through an epithelial transition (EMT) upregulated promotes SF767 line vitro . Results To further define functions we tested impact...
The prediction of the outcome individual patients with glioblastoma would be great significance for monitoring responses to therapy. We hypothesise that, although a large number genetic-metabolic abnormalities occur upstream, there are two 'final common pathways' dominating growth – net rates proliferation (ρ) and dispersal (D). These can estimated from features pretreatment MR images applied in mathematical model predict tumour growth, impact extent resection patient survival. Only...
Abstract Purpose: Hypoxia is associated with resistance to radiotherapy and chemotherapy activates transcription factors that support cell survival migration. We measured the volume of hypoxic tumor maximum level hypoxia in glioblastoma multiforme before [18F]fluoromisonidazole positron emission tomography assess their impact on time progression (TTP) or survival. Experimental Design: Twenty-two patients were studied biopsy between resection starting radiotherapy. Each had a 20-minute scan 2...
The supplementary motor area (SMA) is a region located within each cerebral hemisphere at the posterior mesial border of frontal lobe adjacent to falx. functional significance this has been somewhat unclear, and information regarding its influence on output largely based evoked responses direct stimulation in primates humans. In series patients with primary metastatic tumors involving dominant SMA, distinct pattern postoperative deficits recovery emerged which emphasizes role critical...
TWIST is a basic helix-loop-helix (bHLH) transcription factor that regulates mesodermal development, promotes tumor cell metastasis, and, in response to cytotoxic stress, enhances survival. Our screen for bHLH gene expression rat C6 glioma revealed TWIST. To delineate possible oncogenic role the human central nervous system (CNS), we analyzed message, protein gliomas, normal brain. was detected large majority of glioma-derived lines, gliomas examined. Increased mRNA levels were associated...
Object Malignant gliomas are incurable, primary brain neoplasms noted for their potential to extensively invade parenchyma. Current methods of clinical imaging do not elucidate the full extent invasion, making it difficult predict which, if any, patients likely benefit from gross total resection. Our goal was apply a mathematical modeling approach estimate overall tumor invasiveness on patient-by-patient basis and determine whether resection would improve survival in with relatively less...
A 96-well plate-based microfluidic device enables multiplexed delivery of soluble molecules to organotypic tissue slices.
Periostin is a secreted matricellular protein critical for epithelial-mesenchymal transition and carcinoma metastasis. In glioblastoma, it highly upregulated compared with normal brain, existing reports indicate potential prognostic functional importance in glioma. However, the clinical implications of periostin expression function related to its therapeutic have not been fully explored. levels patterns were examined human glioma cells tissues by quantitative real-time PCR...
Abstract Background The extent of intratumoral mutational heterogeneity remains unclear in gliomas, the most common primary brain tumors, especially with respect to point mutation. To address this, we applied single molecule molecular inversion probes targeting 33 cancer genes assay both mutations and gene amplifications within spatially distinct regions 14 glial tumors. Results We find evidence regional multiple including TP53 RB1 an anaplastic oligodendroglioma PDGFRA KIT two glioblastomas...
Low-grade gliomas (LGGs) comprise a diverse set of intrinsic brain tumors that correlate strongly with survival. Data on the effect reoperation are sparse.To evaluate patients LGG.Fifty-two consecutive reoperated LGGs treated at University Washington between 1986 and 2004 were identified evaluated in retrospective analysis.The average overall survival (OS) for this cohort was 12.95 ± 0.96 years. The 10-year rate 57%. absence any residual tumor either first or second operation associated...
Intratumoral drug delivery is a promising approach for the treatment of glioblastoma multiforme (GBM).
We have developed a digitally-manufacturable microfluidic platform that allows for multiplexed drug testing of intact tumor slices.
Abstract Current methods to assess the drug response of individual human cancers are often inaccurate, costly, or slow. Functional approaches that rapidly and directly patient cancer tissue drugs small molecules offer a promising way improve testing, have potential identify best therapy for patients. We developed digitally manufactured microfluidic platform multiplexed testing intact slice cultures, demonstrate use this evaluate responses in cultures from glioma xenografts tumor biopsies....
Fifty-one adult patients with recurrent malignant gliomas were treated in a Phase II trial of multidrug chemotherapy (6-thioguanine, dibromodulcitol, procarbazine, 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea, 5-fluorouracil, and hydroxyurea). Thirty-one underwent radical tumor debulking, before the administration chemotherapy. Fifty-seven percent all had either an objective radiographic response or stabilization disease after institution therapy. The overall median survival time (MST) was...
Chordomas of the skull base are locally aggressive neoplasms for which maximal surgical resection confers prolonged survival.To present largest consecutive series cranial chordomas to date, including complications, functional outcome, and overall (OS) recurrence-free survival (RFS) in early late eras our experience.From 1988 2011, 95 patients with were treated, 56 from 1999 39 2000 2011. Mean age average follow-up 42.6 ± 16.8 years 38.3 38.5 months, respectively. A historically controlled...
Although neurogenesis occurs in discrete areas of the adult mammalian brain, neural progenitor cells (NPCs) produce fewer new neurons with age. To characterize molecular changes that occur during aging, we performed a proteomic comparison between primary-cultured NPCs from young and aged mouse forebrain. This analysis yielded proteins necessary for cellular metabolism. Mitochondrial quantity oxygen consumption rates decrease although mitochondrial DNA does not have increased mutation rates....
First invoked by Paget, the seed and soil hypothesis suggests that successful growth of metastatic cells depends on interactions properties cancer (seeds) their potential target organs (soil). In context this review examines recent advances in understanding molecular cellular features permit transformed epithelial to gain access blood stream (intravasation), survive journey through stream, ultimately traverse microvasculature (extravsation) deposit, survive, grow a foreign tissue...