A5080721533- Fibroblast Growth Factor Research
- Cell death mechanisms and regulation
- Mitochondrial Function and Pathology
- Cancer-related Molecular Pathways
- Neurogenesis and neuroplasticity mechanisms
- Advanced Proteomics Techniques and Applications
- Histone Deacetylase Inhibitors Research
- Nerve injury and regeneration
- Neuroinflammation and Neurodegeneration Mechanisms
- Alzheimer's disease research and treatments
- Proteoglycans and glycosaminoglycans research
- Axon Guidance and Neuronal Signaling
- Ubiquitin and proteasome pathways
- Nuclear Receptors and Signaling
- Neuroscience and Neuropharmacology Research
- RNA Research and Splicing
- Cancer, Hypoxia, and Metabolism
- Mass Spectrometry Techniques and Applications
- Hippo pathway signaling and YAP/TAZ
- Kruppel-like factors research
- Cancer Research and Treatments
- Epigenetics and DNA Methylation
- HIV Research and Treatment
- RNA Interference and Gene Delivery
- Cholinesterase and Neurodegenerative Diseases
Foso College of Education
2022-2023
Hamilton Medical Center
2023
University of Washington
2012-2022
Neurological Surgery
2009-2021
EDF Energy (United Kingdom)
2021
DRG Undersea Consulting (United States)
2015
Ames Research Center
2010
United States Geological Survey
2006
National Cancer Institute
2004
Science Applications International Corporation (United States)
2004
Bovine basic fibroblast growth factor (bFGF) is a potent mitogen isolated from bovine pituitary glands and brain. The addition of homogeneous bFGF to primary cultures rat cerebral cortical neurons markedly enhances cell survival elaboration neurites. These effects are dose-dependent, with optimal stimulation occurring at concentration 500 pg/ml. Maintenance neurite outgrowth require the continuous presence bFGF. Other factors, such as thrombin, platelet-derived factor, beta nerve interleukin...
Nitric oxide is a chemical messenger implicated in neuronal damage associated with ischemia, neurodegenerative disease, and excitotoxicity. Excitotoxic injury leads to increased NO formation, as well stimulation of the p38 mitogen-activated protein (MAP) kinase neurons. In present study, we determined if NO-induced cell death neurons was dependent on MAP activity. Sodium nitroprusside (SNP), an donor, elevated caspase activity induced human SH-SY5Y neuroblastoma cells primary cultures...
The tumor suppressor gene p53 has been implicated in the loss of neuronal viability, but signaling events associated with p53-mediated cell death cortical and hippocampal neurons are not understood. Previous work shown that adenovirus-mediated delivery causes some features typical apoptosis. In present study we determined whether p53-initiated changes viability were dependent on members Bcl-2 family regulators. Primary cultures derived from animals containing Bax (+/+ +/-) or those deficient...
Epidermal growth factor (EGF) is a potent polypeptide mitogen originally isolated from the adult male mouse submaxillary gland. It also acts as gastrointestinal hormone. EGF-immunoreactive material has recently been identified within neuronal fibers and terminals in rodent brain. In present study, EGF was found to enhance survival process outgrowth of primary cultures subneocortical telencephalic neurons neonatal rat brain dose-dependent manner. This effect observed with concentrations low...
Astrocytes purified from primary cultures of neonatal rat cerebrum can now be grown in a synthetic medium supplemented with putrescine, prostaglandin F2 alpha, insulin, fibroblast growth factor, and hydrocortisone. These five supplements have marked synergistic effect on when used combination but little individually. the defined exhibit dramatic changes morphological characteristics comparison to cells serum-free or serum-supplemented medium. In addition, these express astrocyte-specific...
Epidermal growth factor (EGF) is a potent mitogen with hormonal activity in the gastrointestinal tract. Material cross-reacting EGF was detected central nervous system of developing and adult albino rat by indirect immunofluorescence technique. High concentrations EGF-cross-reacting material were identified forebrain midbrain structures pallidal areas brain. These include globus pallidus, ventral pallidum, entopeduncular nucleus, substantia nigra pars reticulata, islands Calleja . Thus, may...
A role for p53-related modulation of neuronal viability has been suggested by the finding that p53 expression is increased in damaged neurons models ischemia and epilepsy. These findings were recently extended with demonstration mice deficient ("knock-out" mice) exhibit almost complete protection from seizure-induced brain injury, whereas wild-type display significant cell loss hippocampus other regions. Because knock-out used latter study expressed a global deficiency all types, it was not...
Malignant astrocytomas, which are highly invasive, vascular neoplasms, compose the majority of nervous system tumors in humans. Elevated expression fibroblast growth factors (FGFs) astrocytomas has implicated FGF family mitogens initiation and progression astrocyte-derived tumors. In this study, we demonstrated that human undergo parallel changes FGF-receptor (FGFR) during their from a benign to malignant phenotype. FGFR type 2 (BEK) was abundant normal white matter all low-grade but not...
Abstract Binding of 125 I‐epidermal growth factor (EGF) to purified populations rat astrocytes, oligodendrocytes, and neurons was measured. Astrocytes bound 40,000–100,000 EGF molecules per cell, while oligodendrocytes only 6,000–10,000 cell. In contrast, had little or no capacity bind I‐EGF. alone able stimulate incorporation tritiated thymidine fivefold in astrocyte cultures incubated serum‐free medium. When added the previously described chemically defined medium for astrocytes equivalent...
Abstract Glial fibrillary acidic protein (GFAP) is the major constituent of glial filaments and restricted within CNS to astrocytes. As with other classes intermediate filament proteins, regulation GFAP expression poorly understood. Utilizing highly purified cultures astrocytes a chemically defined (CD) medium, we have demonstrated that subject by hormones growth factors. The concentration GFAP/mg was induced 2–4‐fold in presence hydrocortisone, putrescine, prostaglandin F‐2α (PGF 2α ),...
Herbarium specimens of all except one the 168 recognized species Alyssum Linnaeus have been analysed for their nickel content in order to identify hyperaccumulators ( > 1000 μg per g dry mass) nickel. A further 31 (all section Odontarrhena) were discovered addition 14 European reported earlier. Pot trials on non-accumulator A. serpyllifolium Desfontaines and hyperaccumulator pintodasilvae Dudley ed. involving medium which plants growing, showed that not Odontarrhena able act as...
TWIST is a basic helix-loop-helix (bHLH) transcription factor that regulates mesodermal development, promotes tumor cell metastasis, and, in response to cytotoxic stress, enhances survival. Our screen for bHLH gene expression rat C6 glioma revealed TWIST. To delineate possible oncogenic role the human central nervous system (CNS), we analyzed message, protein gliomas, normal brain. was detected large majority of glioma-derived lines, gliomas examined. Increased mRNA levels were associated...
We tested the hypothesis that glaucoma disrupts electrophysiological conduction properties and axon function in optic nerve as a of intraocular pressure (IOP) levels age DBA/2J mouse model glaucoma. The amplitude integral electrical signals evoked along axons decreased considerably by 6 months increasing IOP levels. At young ages, raised was directly associated with increased vulnerability to metabolic challenge. Changes physiological nerves were accentuated aging, leading loss compound...
Decreased ATP correlates with intraocular pressure exposure in the optic nerves of mice glaucoma. To understand what underlies this energy deficit, we examined mitochondria myelinated nerve axons DBA/2J mouse, a model glaucoma secondary to iris pigment disease, and DBA/2(wt-gpnmb) control strain.Mitochondrial length, width, surface area, health status were measured 30 electron microscopic fields within portion from at 3, 6, 10 months age. Protein was isolated for analysis PINK1, Parkin,...
Building on previous National Institutes of Health-sponsored symposia hydrocephalus research, "Opportunities for Hydrocephalus Research: Pathways to Better Outcomes" was held in Seattle, Washington, July 9-11, 2012. Plenary sessions were organized into four major themes, each with two subtopics: Causes (Genetics and Pathophysiological Modifications); Diagnosis (Biomarkers Neuroimaging); Treatment (Bioengineering Advances Surgical Treatments); Outcome (Neuropsychological Neurological)....
Basic fibroblast growth factor (bFGF) is a heparinbinding protein expressing potent mitogenic and angiogenic properties.Elevated levels of bFGF have recently been described in human glioma cell lines.The high degree vascularity invasiveness which characterize gliomas suggest that activated expression or similar proteins may be related to the aberrant patterns these tumors.The influence endogenous on vitro was evaluated present study by downregulating using antisense oligonucleotide...
Spinocerebellar ataxia (SCA) type 7 is an inherited neurodegenerative disorder caused by expansion of a polyglutamine tract within the ataxin-7 protein. To determine molecular basis neurotoxicity in this and other related disorders, we produced SCA7 transgenic mice that express with 24 or 92 glutamines all neurons CNS, except for Purkinje cells. Transgenic expressing (92Q) developed dramatic neurological phenotype presenting as gait culminating premature death. Despite absence expression...