A5068717107- Cancer Research and Treatments
- Virus-based gene therapy research
- Gastric Cancer Management and Outcomes
- RNA Interference and Gene Delivery
- Pancreatic and Hepatic Oncology Research
- Nanoplatforms for cancer theranostics
- Cancer, Hypoxia, and Metabolism
- Esophageal and GI Pathology
- Esophageal Cancer Research and Treatment
- Cancer Cells and Metastasis
- Gastrointestinal Tumor Research and Treatment
- Cancer-related Molecular Pathways
- CAR-T cell therapy research
- Photodynamic Therapy Research Studies
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Genetic factors in colorectal cancer
- Metastasis and carcinoma case studies
- CRISPR and Genetic Engineering
- Colorectal Cancer Treatments and Studies
- Cancer Immunotherapy and Biomarkers
- Organ Transplantation Techniques and Outcomes
- Hepatocellular Carcinoma Treatment and Prognosis
- Cancer Genomics and Diagnostics
- Nutrition and Health in Aging
- Immunotherapy and Immune Responses
Okayama University
2016-2025
Okayama University Hospital
2012-2025
Japan Society of Laparoscopic Colorectal Surgery
2024
Okayama Shoka University
2012-2024
Pharmaceutical Biotechnology (Czechia)
2013-2020
Kawasaki Medical School
2020
American Gastroenterological Association
2012-2018
Ministry of Education, Culture, Sports, Science and Technology
2017
Ministry of Health Labour and Welfare
2017
Family Inada (Japan)
2015
Fas/APO-1 is a cell surface protein known to trigger apoptosis upon specific antibody engagement. Because wild-type p53 can activate transcription as well induce apoptosis, we queried whether might upregulate Fas/APO-1. To explore this possibility, examined human p53-null (H358 non-small-cell lung adenocarcinoma and K562 erythroleukemia) p53-containing (H460 adenocarcinoma) lines. When H358 or H460 cells were transduced with replication-deficient adenovirus expression construct containing...
Purpose: Colorectal cancer is one of the most common malignancies worldwide. Recently, a novel circular RNA, ciRS-7, was proposed to be potential miR-7 sponge. As miR-7, putative tumor-suppressor, regulates expression several important drivers colorectal cancer, we analyzed clinical significance ciRS-7 in patients.Experimental Design: Initially, evaluated levels training cohort comprising 153 primary tissues and 44 matched normal mucosae. We subsequently confirmed its relevance an...
Purpose: Cancer-associated fibroblasts (CAFs) in the tumor microenvironment (TME) play a central role progression. We investigated whether CAFs can regulate tumor-infiltrating lymphocytes (TILs) and their immunosuppression.Experimental Design: A total of 140 cases esophageal cancer were analyzed for CD8+ or forkhead box protein 3 (FoxP3+) TILs by IHC. cytokines using murine human cells. Murine-derived cells also inoculated into BALB/c BALB/c-nu/nu mice tumors treated with recombinant IL6...
Emerging evidence suggests that PIWI-interacting RNAs (piRNAs) may be important epigenetic regulators of gene expression in human cancers; however, their functional and clinical significance colorectal cancer (CRC) remains unknown. We performed piRNA profiling paired normal tissues through small RNA-sequencing. The candidate piRNAs was investigated, independently validated 771 CRC patients from three independent cohorts. biological function characterized cell lines, followed by...
Colony-stimulating factor 1 (CSF1) is a primary regulator of the survival, proliferation, and differentiation monocyte/macrophage that sustains protumorigenic functions tumor-associated macrophages (TAMs). Considering current advances in understanding role inflammatory tumor microenvironment, targeting components sarcoma such as TAMs, viable strategy. Here, we investigated effect PLX3397 (pexidartinib) potent inhibitor CSF1 receptor (CSF1R). was recently approved by Food Drug Administration...
Importance Evidence of implementation laparoscopic gastrectomy for locally advanced gastric cancer is currently insufficient, as the primary end point in previous prospective studies was evaluated at a median follow-up time 3 years. More robust evidence necessary to verify noninferiority gastrectomy. Objective To compare 5-year survival outcomes between laparoscopy-assisted distal (LADG) and open (ODG) with D2 lymph node dissection cancer. Design, Setting, Participants This multicenter,...
Because of a critical shortage in suitable organs, many patients with terminal liver disease die each year before transplantation can be performed. Transplantation isolated hepatocytes has been proposed for the temporary metabolic support awaiting or spontaneous reversion their disease. A major limitation this form therapy is present inability to isolate an adequate number transplantable hepatocytes. highly differentiated cell line, NKNT-3, was generated by retroviral transfer normal primary...
A phase I clinical trial was conducted to determine the safety of Telomelysin, a human telomerase reverse transcriptase (hTERT) promoter driven modified oncolytic adenovirus, in patients with advanced solid tumors. single intratumoral injection (IT) Telomelysin administered three cohorts (1 x 10(10), 1 10(11), 10(12) viral particles). Safety, response and pharmacodynamics were evaluated. Sixteen variety tumors enrolled. IT well tolerated at all dose levels. Common grade 2 toxicities included...
A role for p53-related modulation of neuronal viability has been suggested by the finding that p53 expression is increased in damaged neurons models ischemia and epilepsy. These findings were recently extended with demonstration mice deficient ("knock-out" mice) exhibit almost complete protection from seizure-induced brain injury, whereas wild-type display significant cell loss hippocampus other regions. Because knock-out used latter study expressed a global deficiency all types, it was not...
Abstract Purpose: Replication-selective tumor-specific viruses present a novel approach for treating neoplastic disease. These vectors are designed to induce virus-mediated lysis of tumor cells after selective viral propagation within the tumor. Telomerase activation is considered be critical step in carcinogenesis, and its activity closely correlated with human telomerase reverse transcriptase (hTERT) expression. We investigated antitumor effect hTERT-specific replication-competent...
PURPOSE: To determine the effectiveness of computed tomography (CT)-guided radiofrequency (RF) ablation malignant thoracic tumors. MATERIALS AND METHODS: CT-guided RF ablations 99 tumors (3–80 mm in largest diameter; mean, 19.5 mm) were performed 35 patients 54 sessions. Ablation was with an generator by using a single internally cooled electrode. Tumors both primary (three lesions) and secondary (pulmonary or pleural metastases, 96 lesions). Follow-up 1–17 months (mean, 7.1 months). CT...
Cancer surgery requires the complete and precise identification of malignant tissue margins including smallest disseminated lesions. Internal green fluorescent protein (GFP) fluorescence can intensely illuminate even single cells but GFP sequence transcription within cell. Introducing selectively activating gene in vivo is made possible by development OBP-401, a telomerase-dependent, replication-competent adenovirus expressing GFP. This potentially powerful adjunct to surgical navigation was...
Adenosine-to-inosine (A-to-I) RNA editing, a process mediated by adenosine deaminases that act on the (ADAR) gene family, is recently discovered epigenetic modification dysregulated in human cancers. However, clinical significance and functional role of editing colorectal cancer (CRC) remain unclear. We have systematically comprehensively investigated expression status ADAR1 levels antizyme inhibitor 1 (AZIN1), one most frequently edited genes cancers, 392 tissues from multiple independent...
// Shuya Yano 1,2,3 , Shukuan Li 1 Qinghong Han Yuying Tan Michael Bouvet 2 Toshiyoshi Fujiwara 3 and Robert M. Hoffman 1,2 AntiCancer, Inc, San Diego, CA Department of Surgery, University California, Gastroenterological Okayama Graduate School Medicine, Dentistry Pharmaceutical Sciences, Okayama, Japan Correspondence: Hoffman, email: Keywords : cell cycle, FUCCI, imaging, S/G phase block, recombinant methioninase, rMETase, chemotherapy, HeLa cells, MCF-7 cells Received July 18, 2014...
Quiescent cancer cells are resistant to cytotoxic agents which target only proliferating cells. Time-lapse imaging demonstrated that tumor-targeting Salmonella typhimurium A1-R (A1-R) decoyed in monolayer culture and tumor spheres cycle from G0/G1 S/G2/M, as by fluorescence ubiquitination-based cell indicator (FUCCI) imaging. infection of FUCCI-expressing subcutaneous tumors growing nude mice also quiescent cells, were the majority tumors, thereby making them sensitive agents. The...
The peritoneum is one of the most frequent metastatic sites in pancreatic cancer patients, and peritoneal dissemination makes this disease refractory due to aggressive progression chemoresistance. Although role tumor microenvironment development recognized, correlation between environment refractoriness remains unclear. intraperitoneal tumor-microenvironment its potential chemo-refractoriness, focusing especially on macrophages, were investigated.Peritoneal washes obtained from cellular...
Lymph node metastasis is a pathognomonic feature of spreading tumors, and overcoming challenge in attaining more favorable clinical outcomes. Esophageal cancer an aggressive tumor for which lymph strong poor prognostic factor, the microenvironment (TME), cancer-associated fibroblasts (CAFs) particular, has been implicated esophageal progression. CAFs play central role TME have reported to provide suitable conditions progression cancer, similar their other malignancies. However, little known...