Poly(glycolic acid) (PGA) has long been a popular polymer in the tissue engineering field. PGA possesses many favorable properties such as biocompatibility, bioabsorbability, and tensile strength. The traditional fiber formation techniques of melt extrusion cold-drawing are generally limited to fibers 10–12 μm diameter. Electrostatic spinning, or electrospinning, is an attractive approach for production much smaller diameter which interest scaffolds. We demonstrate ability control function...

A carbohydrate-binding protein assayed by its ability to agglutinate formalinized sheep erythrocytes is synthesized between 3 and 9 hr after Dictyostelium discoideum cells are deprived of food, as the become cohesive. Agglutination this was inhibited N-acetyl-D-galactosamine, D-galactose, L-fucose, but other monosaccharides had little or no effect. The bound completely Sepharose 4B, isolated in highly purified form elution with D-galactose. It appears be present on surface cohesive not...

Human cardiac progenitor cells (hCPCs) may promote myocardial regeneration in adult ischemic myocardium. The regenerative capacity of hCPCs young patients with nonischemic congenital heart defects for potential use defect repair warrants exploration.Human right atrial specimens were obtained during routine surgery across 3 groups: neonates (age, <30 days), infants 1 month to 2 years), and children >2 ≤13 years). C-kit(+) 3-fold higher than years age. hCPC proliferation was greatest the...

Abstract Binding of 125 I‐epidermal growth factor (EGF) to purified populations rat astrocytes, oligodendrocytes, and neurons was measured. Astrocytes bound 40,000–100,000 EGF molecules per cell, while oligodendrocytes only 6,000–10,000 cell. In contrast, had little or no capacity bind I‐EGF. alone able stimulate incorporation tritiated thymidine fivefold in astrocyte cultures incubated serum‐free medium. When added the previously described chemically defined medium for astrocytes equivalent...

Cell replacement therapy has become an attractive solution for myocardial repair. Typical cell delivery techniques, however, suffer from poor engraftment and inhomogeneous distributions. Therefore, we assessed the hypothesis that epicardially applied, tissue-engineered cardiac patch containing progenitor cells would result in enhanced exogenous engraftment. Human mesenchymal stem (hMSCs) were embedded into a rat tail type I collagen matrix to form patch. Myocardial infarction was induced by...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDiscoidin, a developmentally regulated carbohydrate-binding protein from Dictyostelium discoideium. Purification and characterizationDavid L. Simpson, Steven D. Rosen, Samuel H. BarondesCite this: Biochemistry 1974, 13, 17, 3487–3493Publication Date (Print):August 1, 1974Publication History Published online1 May 2002Published inissue 1 August 1974https://pubs.acs.org/doi/10.1021/bi00714a011https://doi.org/10.1021/bi00714a011research-articleACS...

Human cardiac stem cells (CSCs) promote myocardial regeneration in adult ischemic myocardium. The regenerative capacity of CSCs very young patients with nonischemic congenital heart defects has not been explored. We hypothesized that isolated neonatal-derived may have a higher ability than adult-derived and might address the structural deficiencies disease.Human specimens were obtained during routine surgical procedures from right atrial appendage tissue discarded 2 age groups: neonates...

Clinical and fibreoptic assessment of the positioning laryngeal mask airway was performed in 100 children. observation indicated a patent 98% severe obstruction 2% cases. Perfect positioning, as judged by laryngoscopy, found 49% epiglottis within 49%. Fibreoptic evidence partial 17% not detected clinically.

These studies concern the biological properties of Actinobacillus actinomycetemcomitans leukotoxin in different primates. We compared sensitivity primate polymorphonuclear leukocytes (PMNs) to and determined whether animals had toxin‐neutralizing antibodies serum. In addition, subgingival plaques from representative non‐human primates were analysed for leukotoxin‐producing A. actinomycetemcomitans. PMNs varied significantly leukotoxic extracts Neutrophils humans, Great Apes most Old World...

Article abstract—We treated 16 patients with moderately severe to generalized myasthenia gravis (MG) by immunoadsorption (perfusion through a resin that adsorbs proteins) of 2,500 ml plasma on each four alternate days. Fourteen who completed treatment all had significant improvement in strength (6 excellent, 6 good, and 2 fair), which began mean 42 hours after the first immunoadsorption, reached maximum 4 days fourth (mean, 250% baseline strength), returned over months. Thirty-seven grams...

Tropomodulin is a tropomyosin-binding protein that terminates "pointed-end" actin filament polymerization. To test the hypothesis regulation of tropomodulin:actin stoichiometry critical for maintenance length, tropomodulin levels were altered in cells by infection with recombinant adenoviral expression vectors, which produce either sense or antisense mRNA. Neonatal rat cardiomyocytes infected, and sarcomeric organization was examined. Confocal microscopy indicated overexpression shortened...

Summary Clinical and fibreoplic assessment of positioning the size 1 laryngeal mask airway was performed in 50 infants. A clinically patent obtained 47 patients at first attempt, but perfect positioning, as assessed by fibreoptic laryngoscopy, found only 22 instances. Despite an initially patent, delayed obstruction occurred 12 It is concluded that clinical patency does not guarantee ideal LMA infants, care should be taken to ensure continued because tendency position deteriorate this group children.

Abstract The purpose of this study was to construct a flowmeter that could accurately measure the hydraulic permeability electrospun fibrinogen scaffolds, providing insight into transport properties scaffolds while making measurement their topographical features (fiber diameter and pore size) more accurate. Three different concentrations were used (100, 120, 150 mg/mL) create with three fiber diameters sizes. sizes first analyzed scanning electron microscopy image analysis software. each...

The purpose of this study was to demonstrate that human bladder smooth muscle cells (HBSM) remodel electrospun fibrinogen mats. Fibrinogen scaffolds were and disinfected using standard methods. Scaffolds seeded with 5 × 104 HBSM per scaffold. Cultures supplemented aprotinin concentrations 0 KIU ml−1 (no aprotinin), 100 or 1000 incubated twice weekly media changes. Samples removed for evaluation at 1, 3, 7 14 days. Cultured evaluated a WST-1 cell proliferation assay, scanning electron...

Long-term follow-up of 9 survivors the adult respiratory distress syndrome at 39 to 83 months demonstrated 2 patients with continued improvement their restrictive pattern, 3 stable mild obstructive disease, one patient who had chronic pulmonary disease before deterioration, and 4 without symptoms normal function tests. Attention is drawn bronchial reactivity as defined by a bronchodilator response more than 60 per cent increase in maximal mid-expiratory flow, positive methacholine challenge,...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTβ Elimination and sulfite addition as a means of localization identification substituted seryl threonyl residues in proteins proteoglycansD. L. Simpson, Jovan Hranisavljevic, E. A. DavidsonCite this: Biochemistry 1972, 11, 10, 1849–1856Publication Date (Print):May 9, 1972Publication History Published online1 May 2002Published inissue 9 1972https://pubs.acs.org/doi/10.1021/bi00760a019https://doi.org/10.1021/bi00760a019research-articleACS...

Real-time multimedia streams like audio and video are now integral data types in modern programming environments. Although a great deal of research has investigated effective efficient support for manipulating such although the design digital media "middleware" is fairly well understood, no widely available or commonly accepted model exists within community. The authors believe this lack common practice impedes our collective progress because it prevents disparate groups from easily...

The purified leukotoxin of Actinobacillus actinomycetemcomitans kills human leukemic cell lines (e.g., HL-60, U937, and KG-1) T- B-cell JURKAT, MOLT-4, Daudi, Raji) in a dose- time-dependent manner. 50% effective doses for these are similar to those established polymorphonuclear leukocytes monocytes. In contrast, other nonhuman tumor not susceptible the leukotoxin. These leukemia lymphoid will serve as useful model systems with which study molecular specificity mechanism(s) action actinobacillus

Methylenedioxyamphetamine is a commonly used "street drug", with reputation of providing "good trip". The drug structurally similar to both mescaline and amphetamine. following symptoms were found in patient: sympathomimetic effects, coma, seizures, hyperreflexia, hyperthermia. patient's condition was initially stabilized then deteriorated uncontrollable hyperthermia, hematologic abnormalities, coma that culminated death. Since the hyperthermia based on central peripheral mechanisms,...