A5008633409- DNA and Nucleic Acid Chemistry
- Cancer therapeutics and mechanisms
- DNA Repair Mechanisms
- Metal complexes synthesis and properties
- Cancer-related Molecular Pathways
- Carcinogens and Genotoxicity Assessment
- Bioactive Compounds and Antitumor Agents
- Computational Drug Discovery Methods
- Second Language Learning and Teaching
- Cancer Genomics and Diagnostics
- Microtubule and mitosis dynamics
- Advanced biosensing and bioanalysis techniques
- Bioinformatics and Genomic Networks
- Renal and related cancers
- RNA Interference and Gene Delivery
- Synthesis and Biological Evaluation
- Molecular Biology Techniques and Applications
- Renal cell carcinoma treatment
- EFL/ESL Teaching and Learning
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- Gene expression and cancer classification
- Cell Image Analysis Techniques
- HIV/AIDS drug development and treatment
- Neutropenia and Cancer Infections
National Cancer Institute
2009-2023
Center for Cancer Research
2009-2023
National Institutes of Health
2006-2023
Laboratory of Molecular Genetics
1990-2023
Center for Information Technology
2010-2023
University of Tübingen
1995-2022
Center for Applied Linguistics
2021
University of Maryland, College Park
2013
In Silico Biosciences (United States)
2013
Mayo Clinic
2012
Since 1990, the National Cancer Institute (NCI) has screened more than 60,000 compounds against a panel of 60 human cancer cell lines. The 50-percent growth-inhibitory concentration (GI 50 ) for any single line is simply an index cytotoxicity or cytostasis, but patterns such GI values encode unexpectedly rich, detailed information on mechanisms drug action and resistance. Each compound's pattern like fingerprint, essentially unique among many billions distinguishable possibilities. These...
The method of alkaline elution provides a sensitive measure DNA single-strand length distribution in mamalian cells and is applicable to variety problems concerning damage, repair, replication. physical basis the process was studied. kinetics above transition pH were found occur two phases: an initial phase which rate limiting, followed by accelerated due accumulation alkali-induced strand breaks. range lengths that can be discriminated estimated calibration relative effects x ray, 5 X...
Abstract High-throughput and high-content databases are increasingly important resources in molecular medicine, systems biology, pharmacology. However, the information usually resides unwieldy databases, limiting ready data analysis integration. One resource that offers substantial potential for improvement this regard is NCI-60 cell line database compiled by U.S. National Cancer Institute, which has been extensively characterized across numerous genomic pharmacologic response platforms. In...
Journal Article X-ray induced DNA double strand break production and repair in mammalian cells as measured by neutral filter elution Get access Matthews O. Bradley, Bradley * 1Laboratory of Experimental Pathology, Human Tissue Studies Section, National Cancer Institute, Institutes HealthBethesda, MD 20205, USA *Present address for correspondence reprint requests: Merck Sharp & Dohme Research Laboratories, West Point, PA 19486, Search other works this author on: Oxford Academic PubMed Google...
A remarkable overlap was observed between the gadd genes, a group of often coordinately expressed genes that are induced by genotoxic stress and certain other growth arrest signals, MyD set myeloid differentiation primary response genes. The MyD116 gene found to be murine homolog hamster gadd34 gene, whereas MyD118 gadd45 were represent two separate but closely related Furthermore, gadd34/MyD116, gadd45, MyD118, gadd153 encode acidic proteins with very similar unusual charge characteristics;...
The DNA intercalating agents 4'-(9-acridinyl-amino) methanesulfon-m-anisidide (m-AMSA) and adriamycin were studied by using filter elution methods to measure single-strand breaks (SSB's), DNA-protein cross-links (DPC's), double-stranded (DSB's) in mouse leukemia L1210 cells. Both compounds produced SSB's DPC's at nearly 1:1 ratios. shown be localized with respect each other; this was inferred from the finding that assays based on protein adsorption completely prevented of segments between...
Described here are neural networks capable of predicting a drug's mechanism action from its pattern activity against panel 60 malignant cell lines in the National Cancer Institute's drug screening program. Given six possible classes mechanism, network misses correct category for only 12 out 141 agents (8.5 percent), whereas linear discriminant analysis, standard statistical technique, 20 (14.2 percent). The success net indicates several things. (i) line response patterns rich information...
Ecteinascidin 743 is one of several related marine alkaloids isolated from the Caribbean tunicate Ecteinascidia turbinata. It remarkably active and potent in a variety vitro vivo systems has been selected for development as an anticancer agent. The present study investigates interactions ecteinascidin with DNA. retarded electrophoretic migration both supercoiled relaxed simian virus 40 DNA even presence sodium dodecyl sulfate after ethanol precipitation, consistent covalent modifications....
In an effort to further extend the number of targets for development antiretroviral agents, we have used in vitro integrase assay investigate a variety chemicals, including topoisomerase inhibitors, antimalarial DNA binders, naphthoquinones, flavone quercetin, and caffeic acid phenethyl ester as potential human immunodeficiency virus type 1 inhibitors. Our results show that although several inhibitors--including doxorubicin, mitoxantrone, ellipticines, quercetin--are potent other...