A5103221436- Fungal Infections and Studies
- Antifungal resistance and susceptibility
- Bacillus and Francisella bacterial research
- Animal Disease Management and Epidemiology
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Viral Infections and Immunology Research
- Long-Term Effects of COVID-19
- Nail Diseases and Treatments
- Galectins and Cancer Biology
- Herpesvirus Infections and Treatments
- Glycosylation and Glycoproteins Research
- Vector-Borne Animal Diseases
- Immune Response and Inflammation
- Caveolin-1 and cellular processes
- Animal Virus Infections Studies
- Bacteriophages and microbial interactions
- Cytomegalovirus and herpesvirus research
- Viral gastroenteritis research and epidemiology
- COVID-19 Impact on Reproduction
- Infectious Diseases and Mycology
- Bacterial Genetics and Biotechnology
- Carbohydrate Chemistry and Synthesis
- Forensic Entomology and Diptera Studies
- SARS-CoV-2 detection and testing
Northern California Research
2024
University of California, Davis
2024
Southern Arizona VA Health Care System
2012-2022
National Institute of Veterinary Research
2019-2021
Baylor College of Medicine
2012-2019
University of Arizona
2014-2018
Office of Infectious Diseases
2013
University of Maryland, Baltimore
2012-2013
Methodist Hospital
2012
Texas Children's Hospital
2012
Lumpy skin disease (LSD) is a transboundary, systemic, viral of cattle. The first outbreaks LSD were reported in Lang Son Province Vietnam (bordered to China), and an official document has been submitted OIE on 1 November 2020. Here, we described the genetic profiles this pathogen based four well-known marker regions. virus isolated these was 100% identical viruses China (2019) p32 RP030 genes. Additionally, it very close Russia (2017) p32, RP030, thymidine kinase ORF103 genes (100%, 99.01%,...
Since the first outbreak of African swine fever virus (ASFV) in China 2018, disease has spread to Mongolia, Vietnam, Cambodia, Korea, Laos, Myanmar, Philippines, Timor-Leste, Indonesia and Papua New Guinea. ASFV was officially reported Vietnam on 19 February 2019. The continued occurred whole country within 7 months. phylogenetic analysis showed that ASFVs isolated North Central region belong genotype II serotype 8. Additionally, tandem repeat sequence (TRS) studies indicated these are very...
Since African swine fever virus (ASFV) introduction into Vietnam in 2019, most ASFV strains detected this country belong to the p72 genotype II and intergenic region (IGR) variant. Further investigation of ASFVs isolated Capital Hanoi showed two different variants, IGR I II, which were located between I73R I329L genes strains. This finding suggests co-circulation variants domestic pig population Vietnam.
We previously reported that removal of sialyl residues primed PBMCs to respond bacterial LPS stimulation in vitro. Therefore, we speculated prior desialylation can sensitize the host generate an enhanced inflammatory response upon exposure a TLR ligand, such as LPS, murine model acute lung injury. Intratracheal instillation neuraminidase (NA) 30 min intratracheal administration increased polymorphonuclear leukocytes (PMNs) bronchoalveolar lavage fluid and wet-to-dry weight ratio, measure...
A common finding amongst patients with inhalational anthrax is a paucity of polymorphonuclear leukocytes (PMNs) in infected tissues the face abundant circulating PMNs. major virulence determinant edema toxin (ET), which formed by combination two proteins produced organism, factor (EF), an adenyl cyclase, and protective antigen (PA). Since cAMP, product known to enhance endothelial barrier integrity, we asked whether ET might decrease extravasation PMNs into through closure paracellular...
Background. The specific cellular immunological characteristics of bronchoalveolar lavage (BAL) fluid in acute pulmonary coccidioidomycosis have not been defined. Methods. BAL from patients living a coccidioidomycosis-endemic region Arizona who were undergoing bronchoscopy because infiltrates was analyzed. Mononuclear cells and peripheral blood mononuclear (PBMCs) incubated with the coccidioidal antigen T27K vitro, assays performed. Results. Forty-six studied. Twelve received diagnosis...
Sialic acids on glycoconjugates play a pivotal role in many biological processes. In the airways, sialylated glycoproteins and glycolipids are strategically positioned plasma membranes of epithelia to regulate receptor-ligand, cell-cell, host-pathogen interactions at molecular level. We now demonstrate, for first time, sialidase activity ganglioside substrates human airway epithelia. Of four known mammalian sialidases, NEU3 has substrate preference gangliosides is expressed mRNA protein...
We investigated the ability of a temperate Bacillus anthracis reporter phage (Wβ::luxAB‐2), which transduces bioluminescence to infected cells, detect viable spores from deliberately contaminated environmental water samples. Environmental was inoculated with and assayed Wβ::luxAB‐2. Bioluminescent signals directly correlated input spore concentrations. A limit detection 101 102 CFU per ml within 8 h achieved pond lake water, respectively. Detection greatly simplified by minimizing sample...
ABSTRACT Whereas DNA provides the information to design life and proteins provide materials construct it, metabolome can be viewed as physiology that powers it. As such, metabolomics, field charged with study of dynamic small-molecule fluctuations occur in response changing biology, is now being used basis disease. Here, we describe a comprehensive metabolomic analysis systemic bacterial infection using Bacillus anthracis , etiological agent anthrax disease, model pathogen. An organ blood...
Coccidioidomycosis, commonly known as Valley fever, is a common pneumonia in the southwestern United States. In this paper, we examined release of 30 inflammatory proteins whole-blood samples obtained from persons with coccidioidal after blood were incubated preparation made causative fungus, Coccidioides . We found that six these proteins, all cytokines, specifically released high concentrations patients. Three cytokines seen very early disease, and an assay for might serve marker diagnosis fever.
The rapid and reliable detection of the African swine fever virus (ASFV) plays an important role in emergency control preventive measures ASF. Some methods have been recommended by FAO/OIE to detect ASFV clinical samples, including realtime polymerase chain reaction (PCR). However, mismatches primer probe binding regions may cause a false-negative result. Here, slight modification sequence has conducted improve qualification real-time PCR based on World Organization for Animal Health (OIE)...
ABSTRACT The outcome of coccidioidomycosis depends on a robust specific cellular immune response. A T-helper type 1 (Th1) response has been previously associated with resolution clinical illness. However, the precise elements this and whether cytokines not involved Th1 play role in are known. Whole-blood samples were obtained from subjects active controls incubated for 18 h T27K, coccidioidal antigen preparation. supernatant was then assayed gamma interferon (IFN-γ), interleukin-2 (IL-2),...
ABSTRACT Bacillus anthracis is a sporulating Gram-positive bacterium that the causative agent of anthrax and potential weapon bioterrorism. The U.S.-licensed vaccine made from an incompletely characterized culture supernatant nonencapsulated, toxigenic strain (anthrax absorbed [AVA]) whose primary protective component thought to be antigen (PA). AVA effective in protecting animals elicits toxin-neutralizing antibodies humans, but enthusiasm dampened by its undefined composition, multishot...
To date, many kinds of classical swine fever (CSF) vaccines have been developed to protect against this disease. However, the efficacy these pig field CSF strains needs be considered, based on circulating virus (CSFV). Recombinant E2-CSFV protein produced by baculovirus/insect cell system was analyzed western blots and immunoperoxidase monolayer assay. The effect CSFV-E2 subunit evaluated in experimental pigs with three genotypes CSFV challenge. Anti-E2 specific neutralizing antibodies were...
Summary A challenge common to all bacterial pathogens is acquire nutrients from hostile host environments. Iron an important cofactor required for essential cellular processes such as DNA repair, energy production and redox balance. Within a mammalian host, most iron sequestered within heme, which in turn predominantly bound by hemoglobin. While little understood about the mechanisms hemophores attain heme host‐hemoglobin, even less known intracellular processing. Bacillus anthracis ,...
Abstract Background African swine fever (ASF) is an important disease affecting and has a significant economic loss on both the developed developing world but due to lack of vaccines, drug, effective control measures, ASF virus remains serious threat global pork production. The activities medium-chain fatty acids (MCFAs) against viral pathogens have been reported previously. However, effects this family ASFV not yet investigated. In study, we evaluated potential MCFAs in individual...