A5050783027- Neuroscience of respiration and sleep
- Neuroendocrine regulation and behavior
- Heart Rate Variability and Autonomic Control
- Neuroscience and Neuropharmacology Research
- Neonatal and fetal brain pathology
- Ion channel regulation and function
- Neuropeptides and Animal Physiology
- Pain Mechanisms and Treatments
- Neonatal Respiratory Health Research
- Anesthesia and Neurotoxicity Research
- Infant Health and Development
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Organ Donation and Transplantation
- Nitric Oxide and Endothelin Effects
- Congenital Diaphragmatic Hernia Studies
- Receptor Mechanisms and Signaling
- Cardiac Arrest and Resuscitation
- High Altitude and Hypoxia
- Chronic Kidney Disease and Diabetes
- Adenosine and Purinergic Signaling
- Veterinary Orthopedics and Neurology
- Renal and Vascular Pathologies
- Intensive Care Unit Cognitive Disorders
- Kidney Stones and Urolithiasis Treatments
- Obstructive Sleep Apnea Research
Institute of Biomedical Sciences, Academia Sinica
2006-2022
Zhaoqing University
2020
Taichung Veterans General Hospital
1989-2014
National Defense Medical Center
1992-2013
Academia Sinica
1989-2011
Changhua Christian Hospital
2011
National Yang Ming Chiao Tung University
2007
Taipei Veterans General Hospital
2007
Roche (United States)
2006
University of California, Los Angeles
1975-1998
Development of parasympathetic and sympathetic reflexes controlling heart rate, vascular pressures, blood flows was investigated in fetal lambs weighing 300-5,800 g (65-165 days' gestation). Cardiovascular responses to veratridine injections, atrial stretching, bilateral cervical vagotomy, cholinergic blockade with atropine were used test activities. Responses propranolol phenoxybenzamine beta- alpha-adrenergic Autonomic ganglionic stimulation provided additional information on both...
Systemic and pulmonary hemodynamic responses to adrenergic cholinergic agonists were investigated in fetal lambs between 60 days term gestation. The cardiovascular response these agents increases with age, the increase is related maturation of effector system rather than vascular receptors. bed ductus arteriosus are primary components responding acetylcholine; systemic secondary that occurring lung. Both beds under α-adrenergic control whereas heart β-adrenergic control.
Cytoplasmic polyadenylation element binding protein 2 (CPEB2) is an RNA-binding and translational regulator. To understand the physiological function of CPEB2, we generated CPEB2 knock-out (KO) mice found that most died within 3 d after birth. highly expressed in brainstem, which controls vital functions, such as breathing. Whole-body plethysmography revealed KO neonates had aberrant respiration with frequent apnea. Nevertheless, morphology respiratory rhythm generator diaphragm...
To understand the origination of sympathetic nerve discharge (SND), I developed an in vitro brain stem-spinal cord preparation from neonatal rats. Ascorbic acid (3 mM) was added into bath solution to increase viability preparations. At 24°C, rhythmic SND (recorded splanchnic nerve) consistently observed, but it became quiescent at <16°C. Respiratory-related (rSND) discernible and well correlated with C 4 root activity. Power spectral analysis revealed a dominant 2-Hz oscillation. In most...
Sympathetic nerves conveying central commands to regulate visceral functions often display activities in synchronous bursts. To understand how individual fibers fire synchronously, we establish 'oligofiber recording techniques' record 'several' nerve fiber simultaneously, using vitro splanchnic sympathetic nerve–thoracic spinal cord preparations of neonatal rats as experimental models. While distinct spike potentials were easily recorded from collagenase-dissociated fibers, a problem arising...
An isolated thoracic spinal cord of the neonatal rat in vitro spontaneously generates sympathetic nerve discharge (SND) at ~25°C, but it fails SND genesis ≤ 10°C. Basal levels c-Fos expression cords incubated 10°C and ~25°C were compared to determine anatomical substrates that might participate genesis. Cells exhibited immunoreactivity virtually absent However, c-Fos-positive cells found dorsal laminae, white matter, lamina X, intermediolateral cell column (IML). Cell identities verified by...
Using a splanchnic nerve-spinal cord preparation in vitro that could spontaneously generate sympathetic nerve discharge (SND), we investigated the roles of intraspinal GABA(B) receptors regulation SND. Despite an age-dependent difference sensitivity, bath applications baclofen (Bac; GABA(B)-receptor agonist) consistently reduced SND concentration-dependent manner. The drug specificity Bac activation was verified by application its antagonist saclofen (Sac) or CGP-46381 (CGP). Sac CGP alone...
The T-type calcium channel (T-channel) is a low-voltage-activated channel. Whether T-channels are involved in sympathetic nerve discharge (SND), with subunits α1G and α1H differentially regulating SND genesis, was explored using vitro brainstem-spinal cord-splanchnic preparations of wild-type genetically modified B6 mice. Applications 10-80 μm NNC 55-0396 to block mice reduced concentration-dependent manner. Amounts were measured units signal-to-noise ratio for objective comparisons between...
Ketamine is believed to have sympathomimetic effects, although the central mechanism remains unclear. Using an in vitro splanchnic nerve-spinal cord preparation from neonatal rats, our previous investigations demonstrated that tonic sympathetic activity spontaneously generated thoracic spinal cord. We designed this study investigate whether applications of ketamine would augment and action was dependent on N-methyl-d-aspartate receptors. Bath application significantly reduced a...