A5076709124- Cell Adhesion Molecules Research
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Advanced biosensing and bioanalysis techniques
- DNA and Biological Computing
- Synthesis and biological activity
- Molecular Biology Techniques and Applications
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Synthesis and Characterization of Heterocyclic Compounds
- DNA and Nucleic Acid Chemistry
- Adenosine and Purinergic Signaling
- Inflammatory mediators and NSAID effects
- RNA Research and Splicing
- PARP inhibition in cancer therapy
- Connective tissue disorders research
- Muscle Physiology and Disorders
- TGF-β signaling in diseases
- Gene expression and cancer classification
- COVID-19 diagnosis using AI
- Biosensors and Analytical Detection
- Pharmacological Effects of Natural Compounds
- Virus-based gene therapy research
- Skin and Cellular Biology Research
- Calcium signaling and nucleotide metabolism
University of New Mexico
2021-2025
University of California, San Francisco
2015-2021
Phosphoramidite chemistry remains the industry standard for DNA synthesis despite significant limitations on length and yield of oligonucleotide, time restrictions, hazardous waste production. Herein, we demonstrate single-stranded oligos a solid surface by polymerases reverse transcriptases. We report extension surface-bound oligonucleotides enabled transient hybridization as few two bases to neighboring strand. When multiple structures are possible, each templating different base,...
SARS-CoV-2 has infected over 128 million people worldwide, and until a vaccine is developed widely disseminated, vigilant testing contact tracing are the most effective ways to slow spread of COVID-19. Typical clinical only confirms presence or absence virus, but rather, simple rapid procedure that sequences entire genome would be impactful allow for virus variants, as well appearance new variants. However, traditional short read sequencing methods time consuming expensive. Herein, we...
ABSTRACT Collagen type IV alpha 1 and 2 (COL4A1 COL4A2) are major components of almost all basement membranes. COL4A1 COL4A2 mutations cause a multisystem disorder that can affect any organ but typically involves the cerebral vasculature, eyes, kidneys skeletal muscles. In recent years, patient advocacy family support groups have united under name Gould syndrome. The manifestations syndrome highly variable, animal studies suggest allelic heterogeneity genetic context contribute to clinical...
With over 5.5 million deaths worldwide attributed to the respiratory disease COVID-19 caused by novel coronavirus SARS-CoV-2, it is essential that continued efforts be made track evolution and spread of virus globally. The authors previously presented a rapid cost-effective method sequence entire SARS-CoV-2 genome with 95% coverage 99.9% accuracy. This advantageous for identifying tracking variants in compared traditional short-read sequencing methods which can time-consuming costly. Herein,...
Abstract Spatial transcriptomics enables analysis of gene expression that is spatially resolved within a tissue section, making it possible to elucidate the relationship between individual cells context tissue. This transformative technology researchers better understand function health tissue, developmental processes, and disease. In this study, we present an innovative spatial data using high-resolution DNA chip with total capture region size 6.5 x mm containing 2 µm features for barcoding...
<title>Abstract</title> Background Viral outbreaks, including Dengue, Zika, Ebola, and particularly SARS-CoV-2, have caused significant global impacts unprecedented losses of life. in particular, continues to be a leading cause death worldwide the United States, with many individuals experiencing prolonged symptoms. In this study, we present novel genomic surveillance approach that combines stack-ensembled neural network microarray genome resequencing by hybridization. Results The features ~...
Abstract Enzymatic oligonucleotide synthesis (EOS) has been attempted in many iterations for more than forty years, but chemical remains the industry standard despite hazardous waste produced, time restrictions, and length limitations of approximately 200 bases. Herein, we demonstrate that single-stranded oligos on a solid surface can transiently hybridize to neighboring strands these structures be recognized extended by DNA polymerases reverse transcriptases through mechanism describe as...
ABSTRACT Collagen type IV alpha 1 and 2 (COL4A1 COL4A2) are major components of almost all basement membranes. COL4A1 COL4A2 mutations cause a multisystem disorder called Gould syndrome which can affect any organ but typically involves the cerebral vasculature, eyes, kidneys skeletal muscles. The manifestations highly variable animal studies suggest that allelic heterogeneity genetic context contribute to clinical variability. We previously characterized mouse model caused by Col4a1 mutation...
ABSTRACT With over three million deaths worldwide attributed to the respiratory disease COVID-19 caused by novel coronavirus SARS-CoV-2, it is essential that continued efforts be made track evolution and spread of virus globally. We previously presented a rapid cost-effective method sequence entire SARS-CoV-2 genome with 95% coverage 99.9% accuracy. This advantageous for identifying tracking variants in when compared traditional short read sequencing methods which can time consuming costly....
The VirusHunter ARTIC SARS-CoV-2 Whole Genome Sequencing Assay uses a modified version of the sample prep method developed by Josh Quick and Network using same multiplex RT-PCR primers (https://www.protocols.io/view/ncov-2019-sequencing-protocol-v3-locost-bh42j8ye). protocol begins with two different primer pools for each sample. are then combined products fragmented DNase I to generate small fragments that can more efficiently hybridize QuadCore DNA chips from Centrillion Technologies....