A5080210907- Bacteriophages and microbial interactions
- Advanced Electron Microscopy Techniques and Applications
- Advanced X-ray Imaging Techniques
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Enzyme Structure and Function
- SARS-CoV-2 and COVID-19 Research
- Escherichia coli research studies
- Vibrio bacteria research studies
- Viral gastroenteritis research and epidemiology
- Particle Accelerators and Free-Electron Lasers
- Alzheimer's disease research and treatments
- Cell Image Analysis Techniques
- Genetic Neurodegenerative Diseases
- COVID-19 Clinical Research Studies
- Mitochondrial Function and Pathology
- Genomics and Phylogenetic Studies
- Crystallography and Radiation Phenomena
- CRISPR and Genetic Engineering
- Electron Spin Resonance Studies
- Fungal and yeast genetics research
- Photosynthetic Processes and Mechanisms
- Evolution and Genetic Dynamics
- Radiomics and Machine Learning in Medical Imaging
- Prion Diseases and Protein Misfolding
École Polytechnique Fédérale de Lausanne
2012-2024
Centre d'Imagerie BioMedicale
2024
University of Lausanne
2022-2023
University of Geneva
2022
University of Basel
2017-2019
ABSTRACT Bacteriophage phi92 is a large, lytic myovirus isolated in 1983 from pathogenic Escherichia coli strains that carry polysialic acid capsule. Here we report the genome organization of phi92, cryoelectron microscopy reconstruction its virion, and reinvestigation host specificity. The consists linear, double-stranded 148,612-bp DNA sequence containing 248 potential open reading frames 11 putative tRNA genes. Orthologs were found for 130 predicted proteins. Most virion proteins showed...
Abstract Despite the strong evidence linking transactive response DNA-binding protein 43 (TDP-43) aggregation to pathogenesis of frontotemporal lobar degeneration with TDP-43, amyotrophic lateral sclerosis and several neurodegenerative diseases, our knowledge sequence structural determinants its neurotoxicity remains incomplete. Herein, we present a new method for producing recombinant full-length TDP-43 filaments that exhibit morphological features similar those brain-derived filaments. We...
Abstract Cryo-transmission electron microscopy (cryo-EM) of frozen hydrated specimens is an efficient method for the structural analysis purified biological molecules. However, cryo-EM and cryo-electron tomography are limited by low signal-to-noise ratio (SNR) recorded images, making detection smaller particles challenging. For dose-resilient samples often studied in physical sciences, ptychography – a coherent diffractive imaging technique using 4D scanning transmission (4D-STEM) has...
Article18 December 2017Open Access Cryo-EM reconstruction of Type VI secretion system baseplate and sheath distal end Sergey Nazarov orcid.org/0000-0002-5240-5849 Focal Area Infection Biology, Biozentrum, University Basel, Switzerland Search for more papers by this author Johannes P Schneider orcid.org/0000-0002-6028-9956 Maximilian Brackmann orcid.org/0000-0003-0079-1693 Kenneth N Goldie orcid.org/0000-0002-7405-0049 Center Cellular Imaging NanoAnalytics, Henning Stahlberg...
The lack of detailed insight into the structure aggregates formed by huntingtin protein (HTT) has hampered efforts to develop therapeutics and diagnostics targeting pathology formation in brain patients with Huntington's disease. To address this knowledge gap, we investigated structural properties vitro-generated fibrils from exon1 cryogenic electron microscopy single-particle analyses. We show that wildtype mutant form nonhelical a polyglutamine amyloid core composed β-hairpins unique...
Investigation of potential hosts the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is crucial to understanding future risks spillover and spillback. SARS-CoV-2 has been reported be transmitted from humans various animals after requiring relatively few mutations. There significant interest in describing how virus interacts with mice as they are well adapted human environments, used widely infection models can infected. Structural binding data mouse ACE2 receptor Spike protein...
Article12 August 2019Open Access Transparent process Diverse roles of TssA-like proteins in the assembly bacterial type VI secretion systems Johannes Paul Schneider orcid.org/0000-0002-6028-9956 Biozentrum, University Basel, Switzerland Search for more papers by this author Sergey Nazarov orcid.org/0000-0002-5240-5849 Ricardo Adaixo Center Cellular Imaging and NanoAnalytics (C-CINA), Martina Liuzzo Peter David Ringel Henning Stahlberg orcid.org/0000-0002-1185-4592 Marek Basler Corresponding...
Common water disinfectants like chlorine have been reported to select for resistant viruses, yet little attention has devoted characterizing disinfection resistance. Here, we investigated the resistance of MS2 coliphage inactivation by dioxide (ClO2). ClO2 inactivates degrading its structural proteins, thereby disrupting ability attach and infect host. ClO2-resistant virus populations emerged not only after repeated cycles followed regrowth but also dilution-regrowth in absence ClO2. The...
Article20 September 2020Open Access Novel features of centriole polarity and cartwheel stacking revealed by cryo-tomography Sergey Nazarov orcid.org/0000-0002-5240-5849 Swiss Institute for Experimental Cancer Research (ISREC), School Life Sciences, Federal Technology Lausanne (EPFL), Lausanne, Switzerland Interdisciplinary Centre Electron Microscopy (CIME), Search more papers this author Alexandra Bezler orcid.org/0000-0001-6443-8405 Georgios N Hatzopoulos orcid.org/0000-0003-4382-4459...
ABSTRACT Investigation of potential hosts the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is crucial to understanding future risks spillover and spillback. SARS-CoV-2 has been reported be transmitted from humans various animals after requiring relatively few mutations.[1] There significant interest in describing how virus interacts with mice as they are well adapted human environments, used widely infection models can infected.[2] Structural binding data mouse ACE2 receptor...
Abstract The CRISPR-guided caspase (Craspase) complex is an assembly of the target-specific RNA nuclease known as Cas7-11 bound to CRISPR (crRNA) and ancillary protein TPR-CHAT (tetratricopeptide repeats (TPR) fused with a CHAT domain). Craspase holds promise tool for gene therapy biomedical research, but its regulation poorly understood. regulates activity via unknown mechanism. In present study, we use cryoelectron microscopy determine structures Desulfonema magnum ( Dm ) gain mechanistic...
Tau protein fibrillization is implicated in the pathogenesis of several neurodegenerative diseases collectively known as Tauopathies. For decades, investigating vitro has required addition polyanions or other co-factors to induce its misfolding and aggregation, with heparin being most commonly used. However, heparin-induced fibrils exhibit high morphological heterogeneity a striking structural divergence from isolated Tauopathies patients' brains at ultra- macro-structural levels. To address...
Abstract The lack of detailed insight into the structure aggregates formed by huntingtin protein has hampered efforts to develop therapeutics and diagnostics targeting pathology formation in brain patients with Huntington’s disease. To address this knowledge gap, we investigated structural properties vitro generated fibrils from exon1 electron cryo-microscopy single-particle analysis. We show that wildtype mutant form non-helical a polygultamine amyloid core composed β-hairpins unique...
Summary Centrioles are polarized microtubule-based organelles that seed the formation of cilia, and which assemble from a cartwheel containing stacked ring oligomers SAS-6 proteins. A cryo-tomography map centrioles termite flagellate Trichonympha spp. was obtained previously, but higher resolution analysis is likely to reveal novel features. Using sub-tomogram averaging (STA) in T. agilis , we delineate architecture centriolar microtubules, pinhead A-C-linker. Moreover, report ∼25 Å maps...
Dynamic nuclear polarization (DNP) is a process achieved by transferring the spin from an unpaired electron to half-spin nuclei in substance of interest through microwave irradiation, effectively enhancing magnetic resonance (NMR) signals substance. The mechanism involved Overhauser-DNP allows be carried out ambient conditions, thus facilitating its technological implementation. Here we show method quantify protein complexes solution exploiting correlation among concentration, enhancement...
My PhD thesis aims to obtain the structure of several bacteriophages, including so-called “jumbo” phages, using electron cryo-microscopy (cryo-EM) and study structural transformation these viruses as they attach infect host cell. We aim understand process cell membrane penetration at molecular level achieve knowledge necessary how genome proteins, which are originally packaged into capsid, delivered This information can be great importance since bacteriophage therapy is a potentially...