A5081725933- Malaria Research and Control
- Toxoplasma gondii Research Studies
- HIV Research and Treatment
- Mosquito-borne diseases and control
- Cytomegalovirus and herpesvirus research
- T-cell and B-cell Immunology
- Parasite Biology and Host Interactions
- Carbohydrate Chemistry and Synthesis
- Drug Transport and Resistance Mechanisms
- Parasitic Infections and Diagnostics
- Chromosomal and Genetic Variations
- Breastfeeding Practices and Influences
- Herpesvirus Infections and Treatments
- Vibrio bacteria research studies
- Sphingolipid Metabolism and Signaling
- Bacteriophages and microbial interactions
- Infant Nutrition and Health
- Fungal Infections and Studies
- Microbial Metabolic Engineering and Bioproduction
- Genetic diversity and population structure
- Antifungal resistance and susceptibility
- Computational Drug Discovery Methods
- Pneumocystis jirovecii pneumonia detection and treatment
- Global Maternal and Child Health
- Hemoglobinopathies and Related Disorders
University of Geneva
2017-2022
Hospital for Tropical Diseases
2016
Centre National de la Recherche Scientifique
2015
Université Claude Bernard Lyon 1
2015
Kenya Medical Research Institute
2011-2012
Plasmodium gene functions in mosquito and liver stages remain poorly characterized due to limitations the throughput of phenotyping at these stages. To fill this gap, we followed more than 1,300 barcoded P. berghei mutants through life cycle. We discover 461 genes required for efficient parasite transmission mosquitoes stage back into bloodstream mice. analyze screen context genomic, transcriptomic, metabolomic data by building a thermodynamic model liver-stage metabolism, which shows major...
Humans respond to foreign antigen by generating plasma Abs and memory B cells (MBCs). The Ab response then declines, sometimes below the limit of detection. In contrast, MBCs are generally thought be long-lived. We tested compared Plasmodium falciparum ( Pf )-specific MBC responses in two populations children: i ) previously exposed children who had documented infections several years ago, but minimal exposure since then; ii persistently living a separate nearby endemic area. found that...
Toxoplasma gondii possesses sphingolipid synthesis capabilities and is equipped to salvage lipids from its host. The contribution of these two routes lipid acquisition during parasite development unclear. As part a complete ceramide pathway, T. expresses serine palmitoyltransferases (TgSPT1 TgSPT2) dihydroceramide desaturase. After deletion genes, we determine their role in vitro vivo acute chronic infection. Detailed phenotyping through lipidomic approaches reveal perturbed sphingolipidome...
Toxoplasma gondii infects virtually any nucleated cell and resides inside a non-phagocytic vacuole surrounded by parasitophorous vacuolar membrane (PVM). Pivotal to the restriction of T. dissemination upon infection in murine cells is recruitment immunity regulated GTPases (IRGs) guanylate binding proteins (GBPs) PVM that leads pathogen elimination. The virulent type I RH strain secretes handful effectors including dense granule protein GRA7, serine–threonine kinases ROP17 ROP18,...
Countries aiming for malaria elimination require a detailed understanding of the current intensity transmission within their national borders. National household sample surveys are now being used to define infection prevalence but these less efficient in areas exceptionally low endemicity. Here we present results indicator survey Republic Djibouti, first sub-Saharan Africa combine parasitological and serological markers malaria, evaluate extent country explore potential elimination. A...
Acetyl-CoA participates in post-translational modification of proteins and central carbon lipid metabolism several cell compartments. In mammals, acetyl-CoA transporter 1 (AT1, also known as SLC33A1) facilitates the flux cytosolic into endoplasmic reticulum (ER), enabling acetylation secretory pathway, concert with activity dedicated acetyltransferases such NAT8. However, involvement ER pool ER-transiting Apicomplexa is unknown. Here, we identified homologs AT1 NAT8 Toxoplasma gondii...
Gene copy number variants (CNVs), which consist of deletions and amplifications single or sets contiguous genes, contribute to the great diversity in Plasmodium falciparum genome. In vitro studies laboratory have revealed their important role parasite fitness phenotypes such as red cell invasion, transmissibility cytoadherence. Studies natural populations indicate that CNVs are also common field thus may facilitate adaptation its local environment. a survey 183 fresh isolates from three...
Abstract Purpose Cryptococcal meningitis is a leading cause of mortality among HIV-infected individuals in sub-Saharan Africa but little known about its treatment and outcomes decentralised HIV outpatient settings. We assessed adherence to guidelines determined predictors survival. Design A computerised laboratory database identified adults with cryptococcal at Family AIDS Care Education Services Nyanza Province, Kenya, between 2005–2009. Medical records were reviewed. Kaplan-Meier survival...
Breastfeeding imparts beneficial immune protection and nutrition to infants for healthy growth, but it is also a route human immunodeficiency virus (HIV) cytomegalovirus (HCMV) infection. In previous studies, we showed that HCMV adversely affects infant development in Africa, particularly with maternal HIV exposure. this study, analyzed risks acquisition of infection from breastfeeding compared HIV-positive HIV-negative mothers. Two cohorts were studied Zambia. (1) hundred sixty-one...
CNVs found in this study and their characteristics. Names of CNVs, type (amplification or deletion), the genes contained within them previous reports literature. (PDF 175Â kb)