A5091136231- Ophthalmology and Eye Disorders
- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Angiogenesis and VEGF in Cancer
- Analytical Chemistry and Chromatography
- Pituitary Gland Disorders and Treatments
- Axon Guidance and Neuronal Signaling
- Meningioma and schwannoma management
- Thyroid Disorders and Treatments
- Cancer, Hypoxia, and Metabolism
- Liver Disease Diagnosis and Treatment
- Cerebral Venous Sinus Thrombosis
- Neuropeptides and Animal Physiology
- Multiple Sclerosis Research Studies
- Chemical Synthesis and Analysis
- Cerebrovascular and Carotid Artery Diseases
- Antimicrobial Peptides and Activities
- Renal and Vascular Pathologies
- Soft tissue tumor case studies
- Genetics and Neurodevelopmental Disorders
- Genomics and Rare Diseases
- Bone Tumor Diagnosis and Treatments
- Advanced Radiotherapy Techniques
- Vascular Malformations Diagnosis and Treatment
- Medical Imaging and Pathology Studies
Medical University of Lublin
2023-2025
University of Geneva
2019-2025
University of Warsaw
2015-2025
Gdańsk University of Technology
2023
Mossakowski Medical Research Institute, Polish Academy of Sciences
2017-2020
Polish Academy of Sciences
2017-2020
Poznan University of Medical Sciences
2009-2019
University Radiology
2009
Akademia Muzyczna im. Ignacego Jana Paderewskiego w Poznaniu
2004
Toxoplasma gondii possesses sphingolipid synthesis capabilities and is equipped to salvage lipids from its host. The contribution of these two routes lipid acquisition during parasite development unclear. As part a complete ceramide pathway, T. expresses serine palmitoyltransferases (TgSPT1 TgSPT2) dihydroceramide desaturase. After deletion genes, we determine their role in vitro vivo acute chronic infection. Detailed phenotyping through lipidomic approaches reveal perturbed sphingolipidome...
Native electrospray ionization mass spectrometry (ESI-MS) is a standard technique for drug–protein screening but may be affected by nonspecific binding. To address this, reference protein and ligand must selected each investigated. We report versatile, informative, high-throughput approach to differentiate specific interactions based on charge state distribution (CSD) changes caused perturbations (e.g., methanol or heat) without the need surrogate. show that binding stabilizes protein–ligand...
Metoprolol is primarily metabolized via the polymorphic cytochrome P450-2D6 (CYP2D6) enzyme, which underlies interindividual variation in conversion rates and may benefit from pharmacogenetics-driven therapy personalization. However, field relies heavily on knowledge of a drug's metabolism, often originating early-phase clinical trials with single-dose administration small samples healthy volunteers. Pharmacogenetics could thus real-world drug metabolism studies. We conducted study for...
Three dimensional printed open port probe (3DP-OPP) and air displacement based liquid handler, were designed optimized using fused deposition modeling (FDM) stereolitography (SLA) 3D printing. The performance of the devices investigated for analysis solid samples with electrospray ionization mass spectrometry (ESI-MS). Direct in less than 1 min without any sample preparation, enabled detection pesticides (azoxtystrobin/imazalil) on fruits peel surface illegal substances (MDMA/MDEA) home-made...
We report a SAR study on branched peptide-like ligands, which gives an important insight into structural requirements for VEGF-A<sub>165</sub>/NRP-1 complex inhibitors.
Using a chimeric collision cell mounted on quadrupole time-of-flight platform, induced dissociation (CID) and electron (EID) were investigated for the LC-MS analysis of low molecular weight compounds including drugs endogenous metabolites. Compared to CID, EID fragmentation [M+H]+ species (10–20 eV) from standard resulted in additional specific informative fragments, mostly due neutral losses and, some cases ring openings. Some analytes, example reserpine vinpocetine, provided characteristic...
Objectives To evaluate methods for the pre-treatment verification of volumetric modulated arc therapy (VMAT) based on percentage gamma passing rate (%GP) and its correlation sensitivity with dosimetric errors (%DE). Methods A total 25 patients prostate cancer 15 endometrial were analysed. The %GP values 2D 3D verifications different acceptance criteria (1%/1 mm, 2%/2 3%/3 mm) obtained using OmniPro Compass. %DE was calculated a planned dose volume histogram (DVH) created in Monaco's...
Fungal phytopathogens represent a large and economically significant challenge to food production worldwide. Thus, the application of biocontrol agents can be an alternative. In present study, we carried out biological, metabolomic, genetic analyses three endophytic isolates from nodules Chamaecytisus albus, classified as Pseudomonas chlororaphis acting antifungal agents. The efficiency their diffusible volatile compounds (VOCs) was verified in antagonistic assays with use soil-borne...
Hypothyroidism (HT) affects millions worldwide and can lead to various lipid disorders. The metabolic complexity the influence of toxic elements in autoimmune non-autoimmune HT subtypes are not fully understood. This study aimed investigate relationships between plasma lipidome, elements, clinical classifications unexposed individuals.
The structure-activity relationship of branched H-Lys(hArg)-Dab-Dhp-Arg-OH sequence analogues, modified with Cys-Asp or Cys at N-terminal amino acids (Lys, hArg), in VEGF-A165/Neuropilin-1 complex inhibition is presented. addition residue led to a 100-fold decrease the IC50 value, compared parent peptide. change occurred regardless coupling free group present main side chain. A few analogues extended by attachment N-terminus several potent NRP-1 peptide ligands documented literature are also...
Neuropilin-1 (NRP-1), the major co-receptor of vascular endothelial growth factor receptor-2 (VEGFR-2), may also independently act with VEGF-A165 to stimulate tumour and metastasis. Therefore, there is great interest in compounds that can block VEGF-A165/NRP-1 interaction. Peptidomimetic type inhibitors represent a promising strategy treatment NRP-1-related disorders. Here, we present synthesis, affinity, enzymatic stability, molecular modeling vitro binding evaluation branched urea–peptide...
The present work describes the application of an in-house developed 3D-printed open port probe (3DP-OPP) with differential ion mobility spectrometry (DMS) mass spectrometry. Targeted quantitative analysis in urine was performed a triple quadrupole spectrometer selected reaction monitoring mode (OPP-DMS-SRM/MS) and illicit pill screening using data independent acquisition (OPP-DMS-SWATH/MS). combination compensation voltage (CoV) scanning DMS modifiers SWATH/MS for MS/MS spectrum generation...
Mass spectrometry (MS) is increasingly used in clinical studies to obtain molecular evidence of chemical exposures, such as tobacco smoke, alcohol, and drugs. This can help verify data retrieved through anamnesis or questionnaires may provide insights into unreported for example those classified the same despite small but possibly relevant differences due contaminants reported exposure compounds. Here, we aimed explore potential untargeted SWATH metabolomics differentiate closely related...
Neuropilin-1 (NRP-1) is a major co-receptor of vascular endothelial growth factor receptor-2 (VEGFR-2). It may also stimulate tumour and metastasis independently VEGF-A165. These functions make VEGF-A165/NRP-1 complex formation its inhibition great interest, where NRP-1 the target for which effective ligands are sought. Design peptide-like inhibitors represent strategy with potential in treatment NRP-1-related disorders. Here, we present synthesis, molecular modelling, structure-activity...
The behavioral responses exerted by spinal administration of the opioid-neurotensin hybrid peptide, PK23, were studied in adult male rats. antinociceptive effect upon exposure to a thermal stimulus, as well tolerance development, was assessed an acute pain model. PK23 chimera at dose 10 nmol/rat produced potent pain-relieving effect, especially after its intrathecal administration. Compared with morphine, this novel compound found possess favourable side profile characterized reduced scratch...
Mass spectrometry imaging (MSI) can provide accurate data containing the spatial distribution of endogenous peptides in tissue sections without previous treatment. One key issues analyzing small samples is establishing a proper technique for mounting and manipulating collected order to avoid contamination sample with optimal cutting temperature (OCT) resin.We present method embedding rat pituitary frozen egg yolk block, which enables its further experiments on matrix-assisted laser...
Squamous cell carcinoma of the skin is second most common cutaneous malignancy. Despite various available treatment methods and advances in noninvasive diagnostic techniques, incidence metastatic squamous rising. Deficiency effective preventive or transformed keratinocytes leads to necessity searching for new anticancer agents. The present study aims evaluate possibility using wool hydrolysates as such Commercially compounds 5-fluorouracil, ingenol mebutate, diclofenac sodium salt were also...
Background: Wound healing complications affect numerous patients each year, creating significant economic and medical challenges. Currently, available methods are not fully effective in the treatment of chronic or complicated wounds; thus, new constantly sought. Our previous studies showed that a peptide designated as PDGF2 derived from PDGF-BB could be promising drug candidate for wound RADA16-I can serve release system bioactive peptides healing. Based on that, this work, we designed...