A5039056911- Wound Healing and Treatments
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- Molecular Biology Techniques and Applications
- Congenital heart defects research
- Genetic factors in colorectal cancer
- Antimicrobial Peptides and Activities
- Glycosylation and Glycoproteins Research
- Bacteriophages and microbial interactions
- Mesenchymal stem cell research
- Congenital Heart Disease Studies
- Epigenetics and DNA Methylation
- Bacterial Genetics and Biotechnology
- Silk-based biomaterials and applications
- Carbohydrate Chemistry and Synthesis
- Tissue Engineering and Regenerative Medicine
- Enzyme Structure and Function
- RNA Interference and Gene Delivery
- Genomic variations and chromosomal abnormalities
- Cancer-related gene regulation
- Mitochondrial Function and Pathology
- Supramolecular Self-Assembly in Materials
- Hydrogels: synthesis, properties, applications
- Animal Genetics and Reproduction
- Enzyme Production and Characterization
Gdańsk University of Technology
2015-2024
GTx (United States)
2017
Gdańsk Medical University
2015
The Wistar Institute
2008-2009
University of Gdańsk
1996-2003
Chemotherapy is a primary method to treat cancer. While chemotherapeutic drugs are designed target rapidly dividing cancer cells, they can also affect other cell types. In the case of dermal cells and macrophages involved in wound healing, cytotoxicity often leads development chronic wounds. The situation becomes even more severe when chemotherapy combined with surgical tumor excision.
Objective: This study evaluated the use of novel peptides derived from platelet-derived growth factor (PDGF-BB) as potential wound healing stimulants. One compounds (named PDGF2) was subjected for further research after cytotoxicity and proliferation assays on human skin cells. Further investigation included evaluation of: migration chemotaxis cells, immunological allergic safety, transcriptional analyses adipose-derived stem cells (ASCs) dermal fibroblasts stimulated with PDGF2, dorsal...
Abstract Self-assembling peptides can be used for the regeneration of severely damaged skin. They act as scaffolds skin cells and a reservoir active compounds, to accelerate scarless wound healing. To overcome repeated administration which healing, we report development three new peptide biomaterials based on RADA16-I hydrogel functionalized with sequence (AAPV) cleaved by human neutrophil elastase short biologically motifs, namely GHK, KGHK RDKVYR. The hybrids were investigated their...
Abstract Adipose-derived stem cells (ASCs) have become an important research model in regenerative medicine. However, there are controversies regarding the impact of prolonged cell culture on ASCs phenotype and their differentiation potential. Hence, we studied 10 clinical replicates from plastic oncological surgery patients, six-passage FBS supplemented cultures. We quantified basic mesenchymal surface marker transcripts encoded proteins after each passage. In parallel, investigated...
Mouse fetuses up to 16 day of embryonic development and nude (Foxn1- deficient) mice are examples animals that undergo regenerative (scar-free) skin healing. The expression transcription factor Foxn1 in the epidermis mouse begins at 16.5 which coincides with transition point from scar-free scar-forming wound In present study, we tested hypothesis is an essential condition establish adult phenotype inactivity keeps immature stage resembling phenomena neoteny. Uninjured C57BL/6J (B6) mice,...
The neonatal murine heart is able to regenerate after severe injury; this capacity however, quickly diminishes and it lost within the first week of life. DNA methylation an epigenetic mechanism which plays a crucial role in development gene expression regulation. Under investigation here are changes patterns accompany loss regenerative potential. MeDIP-chip (methylated immunoprecipitation microarray) approach was used order compare global profiles whole hearts at day 1, 7, 14 56 complemented...
This study presents an innovative method for producing thermosensitive bio-ink from chitosan hydrogels saturated with carbon dioxide and agarose. It focuses on a detailed characterization of their physicochemical properties potential applications in biomedicine tissue engineering. The ORO test approved rapid regeneration the three-dimensional structure chitosan-agarose composites unidirectional bench press simulation test. diffusion dyes through hydrogel membranes strongly depended share...
Epigenetic regulation plays essential role in cell differentiation and dedifferentiation, which are the intrinsic processes involved regeneration. To investigate epigenetic basis of regeneration capacity, we choose DNA methylation as one most important mechanisms MRL/MpJ mouse a model mammalian known to exhibit enhanced response different organs. We report comparative analysis genomic profiles control C57BL/6J mouse. Methylated immunoprecipitation followed by microarray using Nimblegen '3 ×...
BackgroundMost studies on regenerative medicine focus cell-based therapies and transplantations. Small-molecule therapeutics, though proved effective in different medical conditions, have not been extensively investigated research. It is known that healing potential decreases with development developmental changes are driven by epigenetic mechanisms, which suggests repression of capacity.MethodsWe applied zebularine, a nucleoside inhibitor DNA methyltransferases, to stimulate the response...
Aim: Fetal skin is known to heal without scarring. In mice, the phenomenon observed until 16–17 day of gestation – transition from scarless normal healing. The study aims identify key methylome and transcriptome changes following transition. Materials & methods: Methylome profiles were analyzed in murine dorsal using microarray approach. Results conclusion: genes associated with inflammatory response hyaluronate degradation showed increased DNA methylation before transition, while those...
The MRL/MpJ mouse is a laboratory inbred strain known for regenerative abilities which are manifested by scarless closure of ear pinna punch holes. Enhanced healing responses have been reported in other organs. A remarkable feature the that adult retains several embryonic biochemical characteristics, including increased expression stem cell markers. We explored transcriptome heart, liver, spleen, bone marrow and ears. used two reference strains, thus increasing chances to discover genes...
This study presents an innovative method for producing thermosensitive bioink from chitosan hydrogels saturated with carbon dioxide and agarose. It focuses on a detailed characterisation of their physicochemical properties potential applications in biomedicine tissue engineering. The ORO test approved the rapid regeneration three-dimensional structure chitosan–agarose composites unidirectional bench press simulation test. diffusion dyes through hydrogel membranes strongly depended share both...
A novel, universal method for mutation detection utilising the ability of MutS protein to recognise DNA incomplementarities is proposed. The examined and reference fragments are PCR amplified. products purified, mixed, heated cooled form heteroduplexes. In case heteroduplex containing mismatch protected against exonuclease digestion by MutS, while without mismatches degraded. protection effect visualised direct addition a highly sensitive fluorescent dye (SYBR-Gold) selectively binding DNA....
MutS, a DNA mismatch-binding protein, seems to be promising tool for mutation detection. We present three MutS based approaches the detection of point mutations: retardation, protection mismatched against exonuclease digestion, and chimeric proteins. retardation in polyacrylamide gels stained with SYBR-Gold allows using 1-3 microg Thermus thermophilus his6-MutS protein 50-200 ng PCR product. The method enables search broad range from single up several nucleotide, as mutations over...
Regeneration and wound healing are vital to tissue homeostasis organism survival. One of the biggest challenges today’s science medicine is finding methods factors stimulate these processes in human body. Effective solutions promote regenerative responses will accelerate advances engineering, medicine, transplantology, a number other clinical specialties. In this study, we assessed potential efficacy synthetic hexapeptide, RDKVYR, for stimulation repair healing. The hexapeptide marketed...