Although iron deficiency anemia is common, interpreting laboratory test results can be challenging in patients with comorbidities. We aimed to study the accuracy of common biomarkers compared bone marrow staining a large retrospective dataset hematological patients. collected from 6610 (median age 66 years) staining, their concurrent ferritin, transferrin saturation, soluble receptor, transferrin, hemoglobin, and mean red blood cell volume Helsinki University Hospital electronic health...

Laboratory tests to assess novel oral anticoagulants (NOACs) are under evaluation. Routine monitoring is unnecessary, but special circumstances bioactivity assessment becomes crucial. We analyzed the effects of NOACs on coagulation and availability specific assays at different laboratories.Plasma samples spiked with dabigatran (Dabi; 120 300 μg/L) or rivaroxaban (Riva; 60, 146, 305 were sent 115 38 European laboratories, respectively. International normalized ratio (INR) activated partial...

Coagulation disturbances are major contributors to COVID-19 pathogenicity, but limited data exist on the involvement of extracellular vesicles (EVs) and residual cells (RCs). Fifty hospitalized patients stratified by their D-dimer levels into high (>1.5 mg/L,

Abstract Background Extracellular vesicles (EVs) have procoagulative properties. As EVs are known to accumulate in stored blood products, we compared the EV content and coagulation capacity of leukoreduced cold‐stored whole (CSWB) with current prehospital in‐hospital component therapies understand role haemostatic ageing CSWB. Materials Methods Blood was obtained from 12 O RhD‐positive male donors. CSWB therapy red cells (RBCs), OctaplasLG buffy‐coat platelets RBC lyophilized plasma. Samples...

Convalescent plasma (CP) therapy for COVID-19 infection may have favorable safety but varying efficacy, with concerns about its procoagulant impact. We investigated whether administration of CP to hospitalized patients affects their coagulation profile. Fifty-four randomized in a double-blinded fashion received either placebo, low-titer (LCP) or high-titer (HCP). Donor blood samples were obtained at the time plasmapheresis, while recipient collected before infusion, one day post-infusion and...

Abstract Background Non‐refrigerated whole blood can be used for bleeding emergencies when cold‐stored is unavailable. Storage time in room temperature usually limited to 24 h although there little evidence supporting this practice. We studied the quality of stored 5 days investigate effects prolonged storage time. Study Design and Methods Non‐leukoreduced CPDA‐1 from 10 group O or A RhD positive male donors was +22°C 120 h. Samples were taken daily assess cultures, count metabolic...

Anticoagulation therapy has been undergoing a transition during the last decade with progressive availability of direct oral anticoagulants (DOACs). While DOACs are increasingly used in common indications for anticoagulation, including non-valvular atrial fibrillation (AF), deep vein thrombosis (DVT) and pulmonary embolism (PE), traditional anticoagulants, either warfarin or heparins, remain primary choice patients cardiac valvular replacement, severe thrombophilia, pediatric, pregnant many...

Activated clotting time (ACT) is used in cardiac surgery for monitoring unfractionated heparin (UFH). In endovascular radiology, ACT use less established. We aimed to test the validity of UFH radiology. recruited 15 patients undergoing radiologic procedure. was measured with ICT Hemochron® device as point-of-care (1) before standard bolus, (2) immediately after and some cases (3) 1 h into procedure or a combination thereof (altogether 32 measurements). A total two different cuvettes, ACT-LR...

Cold-stored whole blood (CSWB) is increasingly used in damage control resuscitation. Haemostatic function of CSWB seems superior to that reconstituted blood, and it sufficiently preserved for 14-21 days. To provide evidence a yet insufficiently studied aspect prehospital use, we compared vitro haemostatic properties currently in-hospital component therapies.Blood was obtained from 24 O RhD positive male donors. Three products were prepared: CSWB, therapy (red cells [RBCs], OctaplasLG...

Abstract Pathogenic variants of the X‐linked FLNA gene encoding filamin A protein have been associated with a wide spectrum symptoms, including recently described pulmonary phenotype childhood‐onset panlobular emphysema. We describe three female patients from two families novel heterozygous c.5837_2del and c.508C > T. Analysis immunofluorescence peripheral blood smears platelet function was performed for all patients. FLNA‐negative platelets were observed, suggesting that these result in...

Aims Warfarin dose requirement varies significantly. We compared the clinically established doses based on international normalized ratio (INR) among patients with severe thrombosis and/or thrombophilia estimates from genetic dosing algorithms. Methods Fifty requiring permanent anticoagulation, referred to Helsinki University Hospital Coagulation Center, were screened for thrombophilias and genotyped CYP2C9*2 (c.430C>T, rs1799853) , CYP2C9*3 (c.1075A>C, rs1057910) VKORC1 c.‐1639G>A...

BACKGROUND Uncontrollable bleeding is the leading cause of death in traumatically injured patients. The extent to which direct factor Xa inhibitors interfere with applied resuscitation measures presently unknown. STUDY DESIGN AND METHODS In this study, we investigated effect fluids saline, albumin, fresh frozen plasma (FFP) and solvent/detergent (S/D)‐treated plasma, fibrinogen concentrate, prothrombin complex concentrate (PCC), combinations thereof on hemostatic profile...

Coagulation disturbances are common in severe COVID-19 infection. We examined laboratory markers patients during the first wave of pandemic Finland. analysed a wide panel coagulation tests (IL ACL TOP 750/500®) from anonymously collected samples 78 hospitalized intensive care units (ICUs; n = 34) or medical wards (n 44) at Helsinki University Hospital April-May 2020. These data were supplemented with information system results, including complete blood count and C reactive protein (CRP)....

BackgroundThe thrombin generation (TG) assay is a feasible but labor‐intensive method for detecting global coagulation. It enables comprehensive assessment of anticoagulation, while drug‐specific assays assess only exposure. Traditionally, the Calibrated Automated Thrombogram (CAT) has been used, however ST Genesia (Diagnostica Stago) allows automated evaluation.ObjectiveWe aimed to observe coagulation using and compare data with those CAT in anticoagulated patients.Patients methodsIn total,...

Experimental and clinical studies have shown that long term anticoagulation therapy with warfarin can induce vascular calcification which is preventable by vitamin K. Osteoporosis has been to be associated calcification. In the present study, we wanted see, whether INR (International Normalized Ratio), a measure of prothrombin time, serum calcium (corrected albumin) correlate in laboratory data 94 patients on therapy. When adjusted age sex, there was an inverse correlation between two...

Direct oral anticoagulants (DOAC) interfere in laboratory coagulation testing. The aim here was to study how commercial DOAC removal methods, Filter® and DOAC-Stop™, perform eliminate concentrations false positive results lupus anticoagulant (LAC) We acquired 50 patient samples with high of DOACs: apixaban (n = 18, range 68-572 ng/mL), dabigatran 8, 47-154 edoxaban 35-580 ng/mL) rivaroxaban 16, 69-285 ng/mL). DOACs were removed ex vivo either 28) or DOAC-Stop™ 22). Additionally, control...

We studied association of laboratory testing beyond the international normalised ratio (INR) with bleeding and stroke/transient ischaemic attack (TIA) outcomes in patients atrial fibrillation treated warfarin. This was a retrospective nested case-control study from Finnish Warfarin Atrial Fibrillation (FinWAF) registry (n=54 568), reporting management outcome warfarin-anticoagulated patients. Associations blood count test frequency results were assessed together risk or stroke/TIA during...