Abstract Red blood cells (RBCs) are highly deformable and possess a robust membrane that can withstand shear force. Previous research showed in diabetic patients, there is changed RBC ultrastructure, where these elongated twist around spontaneously formed fibrin fibers. These changes may impact erythrocyte function. Ultrastructural analysis of RBCs inflammatory degenerative diseases no longer be ignored should form fundamental tool clinical studies. Consequently, we investigated the...

Progressive renal function decline begins in one third of patients with microalbuminuria and type 1 diabetes. This study examined whether this is associated elevated excretion inflammatory markers urine. Five (IL-6, IL-8, monocyte chemoattractant protein-1, interferon-gamma-inducible protein (IP-10), macrophage protein-1delta) were measured urine samples from the First Joslin Study Natural History Microalbuminuria Type Diabetes, a cohort recruited 1991. Samples obtained 43 participants...

Summary 1. With the aid of isotopie technique, it was shown, that fibrinogen, fibrinogen-fibrin intermediates and fibrinogen degradation products (FDP) may form soluble complexes, which are unclottable by thrombin. 2. These complexes be precipitated protamine sulphate or cooling to 4° C (par a coagulation), 3. The above findings discussed in regard their significance pathology acute defibrination syndrome : a) Paracoagulation without addition thrombin occurs when is caused excessive...

Fibrinogen (FBG) is a high-molecular-weight protein and precursor to the enzymatically formed fibrin. It has been recently discovered that FBG can be converted into an insoluble, fibrin-like polymer by nonenzymatic action of hydroxyl radicals (HRs). These free are generated due reaction between groups water trivalent ferric ions without participation any redox agent. The interaction HRs occurs in purified system, as well human plasma whole blood. Scanning electron microscopy (SEM)...

It has been suggested that oxidative stress and/or mercury compounds play an important role in the pathophysiology of autism.This study compared for first time cerebellar levels marker 3-nitrotyrosine (3-NT), (Hg) and antioxidant selenium (Se) between control autistic subjects.Tissue homogenates were prepared presence protease inhibitors from frozen tissue (n=10; mean age, 15.5 years; PMI, hours) (n=9; age 12.1 19.3 subjects.The concentration 3-NT, determined by ELISA, controls ranged 13.69...

Activation of coagulation pathways results in the formation hemostatic fibrin plugs. Under normal physiologic conditions clots are gradually, albeit completely, degraded by a fibrinolytic enzyme system to ensure proper wound healing and/or blood vessel patency. Yet pathological situations, thrombi not effectively removed, leading chronic thrombosis. The susceptibility enzymatic degradation depends on structure and properties fibers. Many factors have been suspected as culprits, including red...

Introduction: Unliganded iron both contributes to the pathology of Alzheimer's disease (AD) and also changes morphology erythrocytes (RBCs). We tested hypothesis that these two facts might be linked, i.e. RBCs AD individuals have a variant morphology, diagnostic or prognostic value. Methods: included literature survey its relationships vascular system, followed by laboratory study. Four different microscopy techniques were used results statistically compared analyze trends between high...

It is well-known that individuals with increased iron levels are more prone to thrombotic diseases, mainly due the presence of unliganded iron, and thereby production hydroxyl radicals. also known erythrocytes (RBCs) may play an important role during events. Therefore purpose current study was assess whether RBCs had altered morphology in hereditary hemochromatosis (HH), as well some who displayed hyperferritinemia (HF). Using scanning electron microscopy, we assessed means by which RBC...

Aims: Inflammatory diseases associated with iron overload are characterized by a changed coagulation profile, where there is persistent presence of fibrin-like material dense-matted deposits (DMDs). It believed that one source such result the activation blood without generation thrombin, causing clots to become resistant fibrinolytic dissolution. The aim current manuscript therefore apply novel scanning electron microscopy method for assessing role functional chelation in prevention or...

Summary In the present paper described is a simple test for detecting soluble fibrin monomer complexes (SFMC) in blood. The consists mixing 1% protamine sulphate with diluted oxalated plasma or serum and reading optical density at 6190 Å. experiments dog plasma, enriched complexes, it was shown that OD read PS proportional to amount of recovered from precipitate. It found SFMC level increases rabbits infused intravenously thrombin decreases after injection plasmin streptokinase. both cases...

Clumping reaction, using standard suspension of Staph. aureus Newman D-2-C strain and various substrates, was quantitatively tested. It has been shown that clumping occurs in fibrin lysate containing soluble monomer complexes unclottable by thrombin. The reaction positive with staphylococcal strains possessing factor regardless staphylocoagulase production. is similar to paracoagulation induced protamine sulfate. substrate for both reactions stable at 56 degrees C but destroyed 60 C....

Excessive free iron in blood and organ tissues (so called overload) has been observed degenerative diseases such as atherosclerosis, cancer, neurological, certain autoimmune diseases, which fibrin-like deposits are also found. Although most of the body is bound to hemoglobin myoglobin a divalent ferrous form, amount exists trivalent (ferric) ion. This particular chemical state shown be toxic human when not controlled by endogenous and/or dietary chelating agents. Experiments described this...

Blood coagulation under physiological conditions is activated by thrombin, which converts soluble plasma fibrinogen (FBG) into an insoluble clot.The structure of the enzymatically-generated clot very characteristic being composed thick fibrin fibers susceptible to fibrinolytic degradation.However, in chronic degenerative diseases, such as atherosclerosis, diabetes mellitus, cancer, and neurological disorders, clots are different forming dense matted deposits (DMD) that not effectively...

Stroke is associated with the intracerebral formation of fibrin clots which may lead to irreversible brain damage. Thrombolytic therapies employ a variety natural and/or recombinant plasminogen activators initiate fibrinolytic degradation cerebral thrombi. However, such when installed beyond 4- 6-h window, fail achieve expected outcome. This due hydroxyl radical-induced modification fibrin(ogen) molecules rendering them refractory degradation, but no cause increased free radical generation...