A5009462353- Luminescence and Fluorescent Materials
- Analytical Chemistry and Sensors
- Nanoplatforms for cancer theranostics
- Click Chemistry and Applications
- Photodynamic Therapy Research Studies
- Molecular Sensors and Ion Detection
- Photoacoustic and Ultrasonic Imaging
- Porphyrin and Phthalocyanine Chemistry
- Photochemistry and Electron Transfer Studies
- Photoreceptor and optogenetics research
- Photochromic and Fluorescence Chemistry
- Electrochemical Analysis and Applications
- X-ray Diffraction in Crystallography
- Ionic liquids properties and applications
- Radical Photochemical Reactions
- Enzyme function and inhibition
- Cancer, Hypoxia, and Metabolism
- Organic Light-Emitting Diodes Research
- Crystallization and Solubility Studies
- Peptidase Inhibition and Analysis
- Nanoparticle-Based Drug Delivery
- Receptor Mechanisms and Signaling
The University of Tokyo
2017-2024
Kumamoto University
2020
Bunkyo University
2018
Fluorogenic probes for bioimaging have become essential tools life science and medicine, the key to their development is a precise understanding of mechanisms available fluorescence off/on control, such as photoinduced electron transfer (PeT) Förster resonance energy (FRET). Here we establish new molecular design strategy rationally develop activatable fluorescent probes, which exhibit change in response target biomolecules, by controlling twisted intramolecular charge (TICT) process. This...
Various control strategies are available for building fluorogenic probes to visualize biological events in terms of a fluorescence change. Here, we performed the time-dependent density functional theory (TD-DFT) computational analysis twisted intramolecular charge transfer (TICT) process rhodamine dyes. On basis results, designed and synthesized series dyes established quenching strategy that call steric repulsion-induced TICT (sr-TICT), which is greatly accelerated by simple twisting. As...
Photoacoustic (PA) imaging is a novel modality that combines the high contrast of optical and deep tissue penetration ultrasound. PA agents targeting various biological phenomena have been reported, but development activatable probes, which show signal only in presence target molecules, remains challenging spite their potential usefulness for real-time specific biomolecules vivo. To establish simple design strategy we first designed synthesized silicon-rhodamine based near-infrared...
Si-Rhodamines are bright fluorophores with red to near-infrared (NIR) emission, and widely used for fluorescence imaging of biological phenomena. Here, in order extend the scope Si-rhodamine fluorophores, we established a versatile synthesis unsymmetrical Si-rhodamines. To illustrate its value, one these new synthesize far-red NIR probe hypoxia, showed that it can visualize hepatic ischemia mice vivo.
Abstract Near‐infrared (NIR) imaging techniques have attracted significant attention for biological and medicinal applications due to the ability of NIR penetrate deeply into tissues. However, there are very few stable, activatable molecular probes that can utilize light in wavelength range beyond 800 nm. Herein, we report a new system photoacoustic based on tautomeric benziphthalocyanines (BPcs). We found existence free hydroxyl group is crucial absorption BPcs. Synthesized water‐soluble...
Abstract Folate receptors (FRs) are membrane proteins involved in folic acid uptake, and the alpha isoform (FR‐α) is overexpressed ovarian endometrial cancer cells. For fluorescence imaging of FRs vivo, near‐infrared (NIR) region (650–900 nm), which tissue penetration high autofluorescence low, optimal, but existing NIR fluorescent probes targeting FR‐α show non‐specific adsorption, require prolonged washout to visualize tumors. We have designed synthesized a new probe, FolateSiR‐1 ,...
Fluorescence imaging in the near-infrared (NIR) region (650-900 nm) is useful for bioimaging because background autofluorescence low and tissue penetration high this range. In addition, NIR fluorescence as a complementary color window to green red multicolor imaging. Here, we compared photoinduced electron transfer (PeT)-mediated quenching of silicon- phosphorus-substituted rhodamines (SiRs PRs) order guide development improved far-red fluorescent dyes. The results density functional theory...
We developed the cytosolically localized far-red to NIR fluorescent probe for Ca<sup>2+</sup>,<bold>CaSiR-2 AM</bold>, utilizing rhodamine scaffold.
Abstract Folate receptors (FRs) are membrane proteins involved in folic acid uptake, and the alpha isoform (FR‐α) is overexpressed ovarian endometrial cancer cells. For fluorescence imaging of FRs vivo, near‐infrared (NIR) region (650–900 nm), which tissue penetration high autofluorescence low, optimal, but existing NIR fluorescent probes targeting FR‐α show non‐specific adsorption, require prolonged washout to visualize tumors. We have designed synthesized a new probe, FolateSiR‐1 ,...
Aiming at the evaluation of viscosity interfacial solidlike structure ionic liquids (ILs), we performed total internal reflection fluorescence (TIRF) spectroscopy for N,N-diethyl-N′-phenyl-rhodamine (Ph-DER), a fluorescent probe that is sensitive to in high-viscosity range. TIRF spectra glass interface trioctylmethylammonium bis(nonafluorobutanesulfonyl)amide (TOMAC4C4N), hydrophobic IL, showed intensity Ph-DER increases with decrease evanescence penetration depth, suggesting there exists...
Twisted intramolecular charge transfer (TICT) is a phenomenon involving together with rotation upon photoexcitation, and in general this excited state of fluorescent dyes undergoes non-radiative decay (producing no fluorescence). We recently discovered that the magnitude TICT rhodamine derivatives could be regulated by altering size substituents on xanthene moiety, generating differing degrees steric repulsion. To further illustrate usefulness generality strategy, we describe here an...
Abstract Near‐infrared (NIR) imaging techniques have attracted significant attention for biological and medicinal applications due to the ability of NIR penetrate deeply into tissues. However, there are very few stable, activatable molecular probes that can utilize light in wavelength range beyond 800 nm. Herein, we report a new system photoacoustic based on tautomeric benziphthalocyanines (BPcs). We found existence free hydroxyl group is crucial absorption BPcs. Synthesized water‐soluble...
The ever-growing demand for fluorogenic dyes usable in the rapid construction of analyte-responsive fluorescent probes, has recently contributed to a revival interest chemistry diketopyrrolopyrrole (DPP) pigments. In this context, we have explored potential symmetrical and unsymmetrical DPP derivatives bearing two or one 4-pyridyl substituents acting as optically tunable group(s). unique behavior these molecules, closely linked N-substitution/charge state their pyridine unit (i.e., neutral...
The ever-growing demand for fluorogenic dyes usable in the rapid construction of analyte-responsive fluorescent probes, has recently contributed to a revival interest chemistry diketopyrrolopyrrole (DPP) pigments. In this context, we have explored potential symmetrical and unsymmetrical DPP derivatives bearing two or one 4-pyridyl substituents acting as optically tunable group(s). unique behavior these molecules, closely linked N-substitution/charge state their pyridine unit (i.e., neutral...