A5064057828- Plant Molecular Biology Research
- Polysaccharides and Plant Cell Walls
- Plant Reproductive Biology
- Plant Stress Responses and Tolerance
- Postharvest Quality and Shelf Life Management
Durham University
2019-2025
Forward genetic screens play a key role in the identification of genes contributing to plant stress tolerance. Using screen for freezing sensitivity, we have identified novel tolerance gene, SENSITIVE-TO-FREEZING8, Arabidopsis thaliana. We SFR8 using recombination-based mapping and whole-genome sequencing. As was predicted an effect on cell wall composition, used GC-MS polyacrylamide gel electrophoresis measure cell-wall fucose boron (B)-dependent dimerization pectic domain...
Pectin is a major component of plant cells walls. The extent to which pectin chains crosslink with one another determines crucial properties including cell wall strength, porosity, and the ability small, biologically significant molecules access cell. Despite its importance, gaps remain in our comprehension, at molecular level, how cross-links influence mechanical physical This study employs multidisciplinary approach, combining dynamics simulations, experimental investigations, mathematical...
Abstract The Arabidopsis sensitive-to-freezing8 (sfr8) mutant exhibits reduced cell wall (CW) fucose levels and compromised freezing tolerance. To examine whether CW fucosylation also affects the response to desiccation, we tested effect of leaf excision in sfr8 allelic mur1-1. Leaf water loss was strikingly higher than wild type these, but not other, mutants. We hypothesized that guard (GC) walls might limit stomatal closure through altering mechanical properties. Multifrequency atomic...