A5100667090- interferon and immune responses
- RNA modifications and cancer
- RNA Research and Splicing
- Mitochondrial Function and Pathology
- RNA and protein synthesis mechanisms
- Photosynthetic Processes and Mechanisms
- RNA regulation and disease
- Ubiquitin and proteasome pathways
- Image Processing Techniques and Applications
- Cell Image Analysis Techniques
- Viral Infections and Immunology Research
- HIV Research and Treatment
- Viral gastroenteritis research and epidemiology
- Viral Infections and Outbreaks Research
- Algal biology and biofuel production
- Plant-Microbe Interactions and Immunity
- Bacteriophages and microbial interactions
- Machine Learning in Materials Science
MRC University of Glasgow Centre for Virus Research
2024
University of Oxford
2020-2024
Highlights•A third of the RBPome remodels upon SARS-CoV-2 infection•Viral RNPs include 139 cellular and 6 viral RBPs•Inhibition these RBPs hampers infection•The tRNA ligase complex is a key regulator SARS-CoV-2SummarySevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes disease 2019 (COVID-19). relies on RNA-binding proteins (RBPs) to replicate spread, although which control its life cycle remains largely unknown. Here, we employ multi-omic approach identify systematically...
Photosynthesis is the energetic basis for most life on Earth, and in plants it operates inside double membrane–bound organelles called chloroplasts. The photosynthetic apparatus comprises numerous proteins encoded by nuclear organellar genomes. Maintenance of this requires action internal chloroplast proteases, but a role nucleocytosolic ubiquitin-proteasome system (UPS) was not expected, owing to barrier presented double-membrane envelope. Here, we show that photosynthesis (including those...
The backpropagation (BP) algorithm is often thought to be biologically implausible in the brain. One of main reasons that BP requires symmetric weight matrices feedforward and feedback pathways. To address this "weight transport problem" (Grossberg, 1987), two more plausible algorithms, proposed by Liao et al. (2016) Lillicrap (2016), relax BP's symmetry requirements demonstrate comparable learning capabilities on small datasets. However, a recent study Bartunov (2018) evaluate variants...
SUMMARY The initial steps of the human immunodeficiency virus 1 (HIV-1) lifecycle are regulated by cellular RNA-binding proteins (RBPs). To understand scope these early host-virus interactions, we developed in virion RNA interactome capture (ivRIC), which allowed comprehensive and systematic profiling that interact with HIV-1 genomic (g)RNA inside viral particles. ivRIC identified 104 RBPs within encapsidated ribonucleoprotein, many typically found nucleus. Notably, nuclear RBP Rev,...
ABSTRACT Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes COVID-19. SARS-CoV-2 relies on cellular RNA-binding proteins (RBPs) to replicate and spread, although which RBPs control infection remains largely unknown. Here, we employ a multi-omic approach identify systematically comprehensively viral are involved in infection. We reveal that the RNA-bound proteome is remodelled upon infection, having widespread effects RNA metabolic pathways, non-canonical antiviral factors....
SUMMARY The capacity of host cells to sustain or restrict virus infection is influenced by their proteome. Understanding the compendium proteins defining cellular permissiveness key many questions in fundamental virology. Here, we apply a multiomic approach determine that are associated with highly permissive, intermediate, and hostile states. We observed two groups differentially regulated genes: i) robust changes mRNA protein levels, ii) protein/RNA discordances. Many latter classified as...
Abstract Several RNA viruses induce widespread degradation of cellular mRNAs upon infection; however, the biological significance and mechanistic details this phenomenon remain unknown. Here, we make use a model alphavirus, Sindbis virus (SINV), to fill knowledge gap. We found that SINV triggers decay through exonuclease XRN1 5’-to-3’ machinery (5-3DM). These proteins accumulate at viral replication organelles (VROs) interact with non-structural protein 1 (nsP1), bringing mRNA into proximity...
RNA-binding proteins (RBPs) play critical roles in post-transcriptional gene regulation and are known to contribute plant immunity. To understand the responses of cellular RBPs an immune elicitor, we applied RNA interactome capture Arabidopsis leaves treated with flg22. Strikingly, flg22 induced a pervasive remodelling RBPome affecting 186 proteins. Flg22-responsive included classical involved metabolism as well non-canonical RBPs. RBP responders detected after 2h treatment enriched putative...
Photosynthesis is the energetic basis for most life on Earth, and in plants it operates inside double-membrane-bound organelles called chloroplasts. The photosynthetic apparatus comprises numerous proteins encoded by nuclear organellar genomes. Maintenance of this requires action internal chloroplast proteases, but a role nucleocytosolic ubiquitin-proteasome system (UPS) was not expected owing to barrier presented double-membrane envelope. Here, we show that photosynthesis (including those...