A5000796987- RNA Research and Splicing
- RNA modifications and cancer
- RNA regulation and disease
- interferon and immune responses
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Viral Infections and Vectors
- HIV Research and Treatment
- Bacteriophages and microbial interactions
- RNA Interference and Gene Delivery
- Mosquito-borne diseases and control
- Viral Infections and Immunology Research
- Plant Virus Research Studies
- Vector-Borne Animal Diseases
- Cancer-related molecular mechanisms research
- Plant and Fungal Interactions Research
- MicroRNA in disease regulation
- Inflammasome and immune disorders
- Structural Health Monitoring Techniques
- Immune Response and Inflammation
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Immune Cell Function and Interaction
- Environmental and Social Impact Assessments
- Endodontics and Root Canal Treatments
MRC University of Glasgow Centre for Virus Research
2020-2025
Minia University
2022
University of Oxford
2020-2021
Arab Academy for Science, Technology, and Maritime Transport
2020
Uppsala University
2013-2019
Science for Life Laboratory
2014
Highlights•A third of the RBPome remodels upon SARS-CoV-2 infection•Viral RNPs include 139 cellular and 6 viral RBPs•Inhibition these RBPs hampers infection•The tRNA ligase complex is a key regulator SARS-CoV-2SummarySevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes disease 2019 (COVID-19). relies on RNA-binding proteins (RBPs) to replicate spread, although which control its life cycle remains largely unknown. Here, we employ multi-omic approach identify systematically...
At late times during a lytic infection human adenovirus type 5 produces ∼10 8 copies per cell of virus-associated RNA I (VA RNAI). This short highly structured polymerase III transcript has previously been shown to be essential for virus growth. A fraction VA RNAI is processed by Dicer into small RNAs, so-called mivaRNAIs, which are efficiently incorporated the RNA-induced silencing complex. Here, we constructed recombinant adenoviruses with mutations in seed sequence both 5′- and 3′-strand...
Significance There is a strong need for the development of new antiviral therapies, and this study sheds light on host–virus interaction that significant number medically important human viruses. The also suggests RNA-binding zinc finger CCCH-type containing 11A (ZC3H11A) protein takes part in mechanism facilitates nuclear export mRNA, particularly under cellular stress, has been “hijacked” by several nuclear-replicating viruses to promote their replication. ZC3H11A therefore potential...
Abstract Some strains of the inherited bacterium Wolbachia have been shown to be effective at reducing transmission dengue virus (DENV) and other RNA viruses by Aedes aegypti in both laboratory field settings are being deployed for DENV control. The degree inhibition varies between strains. Density tissue tropism can contribute these differences but there also indications that this is not only factor involved: example, w Au AlbA maintained similar intracellular densities produces strong...
RNA is a central molecule for viruses; however, the interactions that viral (vRNA) establishes with host cell only starting to be elucidated. Here, we determine ribonucleoprotein (RNP) composition of prototypical arthropod-borne Sindbis virus (SINV). We show SINV RNAs engage hundreds cellular proteins, including group nuclear RNA-binding proteins (RBPs) unknown roles in infection. demonstrate these RBPs are selectively translocated cytoplasm after infection, where they accumulate replication...
SUMMARY The initial steps of the human immunodeficiency virus 1 (HIV-1) lifecycle are regulated by cellular RNA-binding proteins (RBPs). To understand scope these early host-virus interactions, we developed in virion RNA interactome capture (ivRIC), which allowed comprehensive and systematic profiling that interact with HIV-1 genomic (g)RNA inside viral particles. ivRIC identified 104 RBPs within encapsidated ribonucleoprotein, many typically found nucleus. Notably, nuclear RBP Rev,...
Virus infected immune cells can rapidly respond to the invader by activating inflammasome and as a consequence release proinflammatory cytokines eventually die pyroptosis. In human adenovirus-5 (Ad5) THP-1 cells, inhibition of NLRP3 activation was demonstrated decreased secretion HMGB1 matured forms caspase-1and IL-1ß. An Ad5 mutant virus defective in expression non-coding VA RNAI failed inhibit addition displayed formation ASC specks increased cell lysis. This required phylogenetically...
Human adenoviruses (HAds) encode for one or two highly abundant virus-associated RNAs, designated VA RNAI and RNAII, which fold into stable hairpin structures resembling miRNA precursors. Here we show that the terminal stem of RNAs originating from Ad4, Ad5, Ad11 Ad37, all undergo Dicer dependent processing virus-specific miRNAs (so-called mivaRNAs). We further mivaRNA duplex is subjected to a asymmetric RISC loading with 3′-strand being favored strand, except Ad37 where 5′-mivaRNAII strand...
Adenovirus type 5 encodes two short, highly structured noncoding RNAs, the virus-associated (VA) RNAI and VA RNAII. These RNAs are expressed in large amounts late during a lytic infection. Early studies established an important role for maintaining efficient translation virus-infected cells by blocking activation of key interferon-induced PKR protein kinase. More recent have demonstrated that also target RNAi/miRNA pathway. Collectively, available data suggest multifunctional suppressing...
ABSTRACT Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes COVID-19. SARS-CoV-2 relies on cellular RNA-binding proteins (RBPs) to replicate and spread, although which RBPs control infection remains largely unknown. Here, we employ a multi-omic approach identify systematically comprehensively viral are involved in infection. We reveal that the RNA-bound proteome is remodelled upon infection, having widespread effects RNA metabolic pathways, non-canonical antiviral factors....
Abstract The expansion of tropical mosquito habitats and associated arboviruses is a risk for human health, it thus becomes fundamental to identify new antiviral strategies. In this study we employ approach elucidate the composition ribonucleoproteins (RNPs) prototypical arbovirus called Sindbis (SINV). SINV RNPs contain 453 cellular 6 viral proteins, many these proteins are nuclear in uninfected cells redistribute cytoplasm upon infection. These findings suggest that RNAs act as...
Here we show that the adenovirus major late promoter produces a 31-nucleotide transcriptional start site small RNA (MLP-TSS-sRNA) retains 7-methylguanosine (m7G)-cap and is incorporated onto Ago2-containing RNA-induced silencing complexes (RISC) in human adenovirus-37 infected cells. polymerase II CLIP (UV-cross linking immunoprecipitation) experiments suggest MLP-TSS-sRNA produced by proximal stalling/termination of transcription at 3′ end. The highly stable cells functionally active,...
We have used high-throughput small RNA sequencing to characterize viral expression in purified tonsillar B and T lymphocytes isolated from patients tested positive for Epstein-Barr virus (EBV) or human adenovirus (HAdV) infections, respectively. In the set of analyzed, profile EBV HAdV miRNAs could not distinguish between diagnosed with hypertrophy chronic/recurrent tonsillitis. The miR-BART among diseases resembles most closely pattern seen EBV+ tumors (Latency II/I). miR-BARTs that appear...
The adenovirus L4-22K protein both activates and suppresses transcription from the major late promoter (MLP) by binding to DNA elements located downstream of MLP transcriptional start site: so-called DE element (positive) R1 region (negative). Here we show that preferentially binds RNA form region, double-stranded single-stranded same polarity as nascent transcript. Further, a 5΄-CAAA-3΄ motif in RNA, which is identical sequence characterized for binding. results an enhancement U1 snRNA...
Vegetation and water bodies, known as green blue infrastructure can be an important strategy for shaping sustainable resilient cities. In this study, the effect of on cooling energy use residential buildings in arid hot desert climate is investigated using a coupled simulations methodology combining microclimate, building change simulations. Results revealed that, comparison with baseline scenario (asphalt road), alone has highest savings (8.12%) followed by infrastructures (6.73%) (4.78%)....
Abstract RNA-binding proteins (RBPs) are pivotal for the viral lifecycle, mediating key host-virus interactions that promote or repress virus infection. While these have been largely studied in vertebrate host, no comprehensive analyses of protein-RNA occurring cells derived from infected vector done to date, particularly ticks. Here we systematically identify responses proteome (RBPome) infection with a prototype bunyavirus (Uukuniemi virus; rUUKV) tick and discovered changes activity 283...